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Hepatitis B plasmonic biosensor for the analysis of clinical serum samples
T. Riedel, F. Surman, S. Hageneder, O. Pop-Georgievski, C. Noehammer, M. Hofner, E. Brynda, C. Rodriguez-Emmenegger, J. Dostálek,
Language English Country England, Great Britain
Document type Journal Article, Validation Study
- MeSH
- Acrylamides chemistry MeSH
- Equipment Design MeSH
- Hepatitis B Surface Antigens immunology MeSH
- Hepatitis B blood immunology MeSH
- Humans MeSH
- Limit of Detection MeSH
- Surface Plasmon Resonance instrumentation MeSH
- Surface Properties MeSH
- Antibodies, Viral blood immunology MeSH
- Hepatitis B virus immunology isolation & purification MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Validation Study MeSH
A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.
Biosensor Technologies AIT Austrian Institute of Technology GmbH Muthgasse 11 1190 Vienna Austria
Institute of Macromolecular Chemistry AS CR v v i Heyrovsky Sq 2 162 06 Prague 6 Czech Republic
References provided by Crossref.org
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