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Hepatitis B plasmonic biosensor for the analysis of clinical serum samples
T. Riedel, F. Surman, S. Hageneder, O. Pop-Georgievski, C. Noehammer, M. Hofner, E. Brynda, C. Rodriguez-Emmenegger, J. Dostálek,
Jazyk angličtina Země Anglie, Velká Británie
Typ dokumentu časopisecké články, validační studie
- MeSH
- akrylamidy chemie MeSH
- design vybavení MeSH
- hepatitida B - antigeny povrchové imunologie MeSH
- hepatitida B krev imunologie MeSH
- lidé MeSH
- limita detekce MeSH
- povrchová plasmonová rezonance přístrojové vybavení MeSH
- povrchové vlastnosti MeSH
- protilátky virové krev imunologie MeSH
- virus hepatitidy B imunologie izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- validační studie MeSH
A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.
Biosensor Technologies AIT Austrian Institute of Technology GmbH Muthgasse 11 1190 Vienna Austria
Institute of Macromolecular Chemistry AS CR v v i Heyrovsky Sq 2 162 06 Prague 6 Czech Republic
Citace poskytuje Crossref.org
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- $a A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer.
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- $a Rodriguez-Emmenegger, Cesar $u Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic; DWI - Leibniz Institute for Interactive Materials and Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Forckenbeckstraße 50, 52074 Aachen, Germany. Electronic address: rodriguez@dwi.rwth-aachen.de.
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