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Wound dressing based on chitosan/hyaluronan/nonwoven fabrics: Preparation, characterization and medical applications
RM. Abdel-Rahman, AM. Abdel-Mohsen, R. Hrdina, L. Burgert, Z. Fohlerova, D. Pavliňák, ON. Sayed, J. Jancar,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- antibakteriální látky chemie terapeutické užití MeSH
- buňky NIH 3T3 MeSH
- chitosan chemie terapeutické užití MeSH
- Escherichia coli účinky léků patogenita MeSH
- hojení ran účinky léků MeSH
- kyselina hyaluronová chemie terapeutické užití MeSH
- lidé MeSH
- myši MeSH
- obvazy mikrobiologie MeSH
- Staphylococcus aureus účinky léků patogenita MeSH
- textilie mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Thin layers of chitosan (positively charged)/sodium hyaluronate (negatively charged)/nonwoven fabrics were constructed by polyelectrolyte multilayer pad-dry-cure technique. Pure chitosan (CS) was isolated from shrimp shell and immobilized onto nonwoven fabrics (NWFs) using citric acid (CTA) as cross linker and solvent agents through a pad-dry-cure method. The prepared thin layer of chitosan citrate/nonwoven fabrics (CSCTA/NWFs) were consequently impregnated with hyaluronan (CSCTA/HA/NWFs) in the second path through a pad-dry-cure method. Chitosan/hyaluronan/nonwoven fabrics wound dressing was characterized by different techniques such as FTIR-ATR, TGA and SEM. The antibacterial activity and the cytotoxicity of the dressing sheets were evaluated against Escherichia coli (E. coli) and Streptococcus aureus (S. aureus), mouse fibroblast (NIH-3T3) and keratinocytes (HaCaT) cell lines, respectively. The cell-fabrics interaction was also investigated using fluorescence microscope, based on live/dead staining assay of 3T3 cells. The healing properties of the new wound dressing were evaluated and compared with the control sample.
CEITEC Central European Institute of Technology Brno University of Technology Brno Czech Republic
Chemistry Department Faculty of Science Fayoum University Fayoum Egypt
Department of Physical Electronics Masaryk University Brno Czech Republic
Citace poskytuje Crossref.org
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- $a Abdel-Rahman, Rasha M $u Institute of Organic Chemistry and Technology, Faculty of Chemical Technology, University of Pardubice, Pardubice, Czech Republic. Electronic address: rmmar2008@yahoo.com. $7 gn_A_00000367
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- $a Thin layers of chitosan (positively charged)/sodium hyaluronate (negatively charged)/nonwoven fabrics were constructed by polyelectrolyte multilayer pad-dry-cure technique. Pure chitosan (CS) was isolated from shrimp shell and immobilized onto nonwoven fabrics (NWFs) using citric acid (CTA) as cross linker and solvent agents through a pad-dry-cure method. The prepared thin layer of chitosan citrate/nonwoven fabrics (CSCTA/NWFs) were consequently impregnated with hyaluronan (CSCTA/HA/NWFs) in the second path through a pad-dry-cure method. Chitosan/hyaluronan/nonwoven fabrics wound dressing was characterized by different techniques such as FTIR-ATR, TGA and SEM. The antibacterial activity and the cytotoxicity of the dressing sheets were evaluated against Escherichia coli (E. coli) and Streptococcus aureus (S. aureus), mouse fibroblast (NIH-3T3) and keratinocytes (HaCaT) cell lines, respectively. The cell-fabrics interaction was also investigated using fluorescence microscope, based on live/dead staining assay of 3T3 cells. The healing properties of the new wound dressing were evaluated and compared with the control sample.
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