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Fluorescence Characterization of Gold Modified Liposomes with Antisense N-myc DNA Bound to the Magnetisable Particles with Encapsulated Anticancer Drugs (Doxorubicin, Ellipticine and Etoposide)
S. Skalickova, L. Nejdl, J. Kudr, B. Ruttkay-Nedecky, AM. Jimenez, P. Kopel, M. Kremplova, M. Masarik, M. Stiborova, T. Eckschlager, V. Adam, R. Kizek,
Language English Country Switzerland
Document type Journal Article, Research Support, Non-U.S. Gov't
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PubMed
26927112
DOI
10.3390/s16030290
Knihovny.cz E-resources
- MeSH
- DNA, Antisense chemistry therapeutic use MeSH
- Doxorubicin chemistry therapeutic use MeSH
- Ellipticines chemistry therapeutic use MeSH
- Etoposide chemistry therapeutic use MeSH
- Fluorescence MeSH
- Drug Delivery Systems * MeSH
- Humans MeSH
- Liposomes chemistry therapeutic use MeSH
- Magnetite Nanoparticles chemistry therapeutic use MeSH
- Neoplasms drug therapy MeSH
- N-Myc Proto-Oncogene Protein antagonists & inhibitors genetics MeSH
- Gold chemistry MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg·mL(-1), respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.
References provided by Crossref.org
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- $a Skalickova, Sylvie $u Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. sylvie.skalickova@gmail.com. Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic. sylvie.skalickova@gmail.com.
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- $a Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg·mL(-1), respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.
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