• Je něco špatně v tomto záznamu ?

Fluorescence-based biosensor for monitoring of environmental pollutants: From concept to field application

S. Bidmanova, M. Kotlanova, T. Rataj, J. Damborsky, M. Trtilek, Z. Prokop,

. 2016 ; 84 (-) : 97-105. [pub] 20151208

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17014175

An advanced optical biosensor was developed based on the enzymatic reaction with halogenated aliphatic hydrocarbons that is accompanied by the fluorescence change of pH indicator. The device is applicable for the detection of halogenated contaminants in water samples with pH ranging from 4 to 10 and temperature ranging from 5 to 60°C. Main advantages of the developed biosensor are small size (60×30×190mm(3)) and portability, which together with short measurement time of 1min belong to crucial attributes of analytical technique useful for routine environmental monitoring. The biosensor was successfully applied for the detection of several important halogenated pollutants under laboratory conditions, e.g., 1,2-dichloroethane, 1,2,3-trichloropropane and γ-hexachlorocyclohexane, with the limits of detection of 2.7, 1.4 and 12.1mgL(-1), respectively. The continuous monitoring was demonstrated by repetitive injection of halogenated compound into measurement solution. Consequently, field trials under environmental settings were performed. The presence of 1,2-dichloroethane (10mgL(-1)) was proved unambiguously on one of three potentially contaminated sites in Czech Republic, and the same contaminant was monitored on contaminated locality in Serbia. Equipped by Global Positioning System, the biosensor was used for creation of a precise map of contamination. Concentrations determined by biosensor and by gas chromatograph coupled with mass spectrometer exhibited the correlation coefficient of 0.92, providing a good confidence for the routine use of the biosensor system in both field screening and monitoring.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17014175
003      
CZ-PrNML
005      
20170428120717.0
007      
ta
008      
170413s2016 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.bios.2015.12.010 $2 doi
035    __
$a (PubMed)26725215
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Bidmanova, Sarka $u Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment (RECETOX), Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech Republic; Enantis, spol. s r.o., Kamenice 34, 612 00 Brno, Czech Republic. Electronic address: bidmanova@sci.muni.cz.
245    10
$a Fluorescence-based biosensor for monitoring of environmental pollutants: From concept to field application / $c S. Bidmanova, M. Kotlanova, T. Rataj, J. Damborsky, M. Trtilek, Z. Prokop,
520    9_
$a An advanced optical biosensor was developed based on the enzymatic reaction with halogenated aliphatic hydrocarbons that is accompanied by the fluorescence change of pH indicator. The device is applicable for the detection of halogenated contaminants in water samples with pH ranging from 4 to 10 and temperature ranging from 5 to 60°C. Main advantages of the developed biosensor are small size (60×30×190mm(3)) and portability, which together with short measurement time of 1min belong to crucial attributes of analytical technique useful for routine environmental monitoring. The biosensor was successfully applied for the detection of several important halogenated pollutants under laboratory conditions, e.g., 1,2-dichloroethane, 1,2,3-trichloropropane and γ-hexachlorocyclohexane, with the limits of detection of 2.7, 1.4 and 12.1mgL(-1), respectively. The continuous monitoring was demonstrated by repetitive injection of halogenated compound into measurement solution. Consequently, field trials under environmental settings were performed. The presence of 1,2-dichloroethane (10mgL(-1)) was proved unambiguously on one of three potentially contaminated sites in Czech Republic, and the same contaminant was monitored on contaminated locality in Serbia. Equipped by Global Positioning System, the biosensor was used for creation of a precise map of contamination. Concentrations determined by biosensor and by gas chromatograph coupled with mass spectrometer exhibited the correlation coefficient of 0.92, providing a good confidence for the routine use of the biosensor system in both field screening and monitoring.
650    _2
$a Bacteria $x enzymologie $7 D001419
650    _2
$a biosenzitivní techniky $x přístrojové vybavení $7 D015374
650    _2
$a monitorování životního prostředí $x přístrojové vybavení $7 D004784
650    _2
$a fluorescence $7 D005453
650    _2
$a chlorované uhlovodíky $x analýza $x metabolismus $7 D006843
650    _2
$a hydrolasy $x metabolismus $7 D006867
650    _2
$a fluorescenční spektrometrie $x přístrojové vybavení $7 D013050
650    _2
$a voda $x analýza $7 D014867
650    _2
$a chemické látky znečišťující vodu $x analýza $x metabolismus $7 D014874
655    _2
$a časopisecké články $7 D016428
700    1_
$a Kotlanova, Marketa $u Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment (RECETOX), Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; Enantis, spol. s r.o., Kamenice 34, 612 00 Brno, Czech Republic. Electronic address: KotlanovaM@seznam.cz.
700    1_
$a Rataj, Tomas $u Global Change Research Centre, Academy of Sciences of the Czech Republic, Drasov 470, 664 24 Drasov, Czech Republic. Electronic address: rataj.t@czechglobe.cz.
700    1_
$a Damborsky, Jiri $u Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment (RECETOX), Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech Republic; Enantis, spol. s r.o., Kamenice 34, 612 00 Brno, Czech Republic. Electronic address: jiri@chemi.muni.cz.
700    1_
$a Trtilek, Martin $u Photon Systems Instruments, spol. s r.o., Drasov 470, 664 24 Drasov, Czech Republic. Electronic address: martin@psi.cz.
700    1_
$a Prokop, Zbynek $u Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment (RECETOX), Faculty of Science, Masaryk University, Kamenice 5/A13, 625 00 Brno, Czech Republic; International Clinical Research Center, St. Anne's University Hospital, Pekarska 53, 656 91 Brno, Czech Republic; Enantis, spol. s r.o., Kamenice 34, 612 00 Brno, Czech Republic. Electronic address: zbynek@chemi.muni.cz.
773    0_
$w MED00006627 $t Biosensors & bioelectronics $x 1873-4235 $g Roč. 84, č. - (2016), s. 97-105
856    41
$u https://pubmed.ncbi.nlm.nih.gov/26725215 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20170413 $b ABA008
991    __
$a 20170428121039 $b ABA008
999    __
$a ok $b bmc $g 1200640 $s 974953
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 84 $c - $d 97-105 $e 20151208 $i 1873-4235 $m Biosensors & bioelectronics $n Biosens Bioelectron $x MED00006627
LZP    __
$a Pubmed-20170413
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace