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ZnO/graphite composites and its antibacterial activity at different conditions
K. Dědková, B. Janíková, K. Matějová, K. Čabanová, R. Váňa, A. Kalup, M. Hundáková, J. Kukutschová,
Language English Country Switzerland
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Anti-Bacterial Agents chemistry pharmacology MeSH
- X-Ray Diffraction MeSH
- Photochemical Processes MeSH
- Graphite chemistry pharmacology MeSH
- Microbial Sensitivity Tests MeSH
- Microscopy, Electron, Scanning MeSH
- Nanocomposites chemistry MeSH
- Zinc Oxide chemistry pharmacology MeSH
- Spectrum Analysis, Raman MeSH
- Staphylococcus aureus drug effects MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The paper reports laboratory preparation, characterization and in vitro evaluation of antibacterial activity of ZnO/graphite nanocomposites. Zinc chloride and sodium carbonate served as precursors for synthesis of zinc oxide, while micromilled and natural graphite were used as the matrix for ZnO nanoparticles anchoring. During the reaction of ZnCl2 with saturated aqueous solution of Na2CO3a new compound is created. During the calcination at the temperature of 500 °C this new precursors decomposes and ZnO nanoparticles are formed. Composites ZnO/graphite with 50 wt.% of ZnO particles were prepared. X-ray powder diffraction and Raman microspectroscopy served as phase-analytical methods. Scanning electron microscopy technique was used for morphology characterization of the prepared samples and EDS mapping for visualization of elemental distribution. A developed modification of the standard microdilution test was used for in vitro evaluation of daylight induced antibacterial activity and antibacterial activity at dark conditions. Common human pathogens served as microorganism for antibacterial assay. Antibacterial activity of ZnO/graphite composites could be based on photocatalytic reaction; however there is a role of Zn(2+) ions on the resulting antibacterial activity which proved the experiments in dark condition. There is synergistic effect between Zn(2+) caused and reactive oxygen species caused antibacterial activity.
AGEL Laboratory Zalužanského 1192 15 703 84 Ostrava Vítkovice Czech Republic
TESCAN Brno s r o Libušina třída 1 623 00 Brno Czech Republic
References provided by Crossref.org
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