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Magnetically modified bacterial cellulose: A promising carrier for immobilization of affinity ligands, enzymes, and cells
E. Baldikova, K. Pospiskova, D. Ladakis, IK. Kookos, AA. Koutinas, M. Safarikova, I. Safarik,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
- MeSH
- Acetobacteraceae chemie MeSH
- bakteriální polysacharidy chemie MeSH
- celulosa chemie MeSH
- enzymy imobilizované chemie MeSH
- genciánová violeť chemie MeSH
- imobilizované buňky cytologie MeSH
- indoly chemie MeSH
- magnetismus MeSH
- nosiče léků chemie MeSH
- organokovové sloučeniny chemie MeSH
- Saccharomyces cerevisiae cytologie MeSH
- skot MeSH
- trypsin chemie MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Bacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was magnetically modified using perchloric acid stabilized magnetic fluid. Magnetic bacterial cellulose (MBC) was used as a carrier for the immobilization of affinity ligands, enzymes and cells. MBC with immobilized reactive copper phthalocyanine dye was an efficient adsorbent for crystal violet removal; the maximum adsorption capacity was 388mg/g. Kinetic and thermodynamic parameters were also determined. Model biocatalysts, namely bovine pancreas trypsin and Saccharomyces cerevisiae cells were immobilized on MBC using several strategies including adsorption with subsequent cross-linking with glutaraldehyde and covalent binding on previously activated MBC using sodium periodate or 1,4-butanediol diglycidyl ether. Immobilized yeast cells retained approximately 90% of their initial activity after 6 repeated cycles of sucrose solution hydrolysis. Trypsin covalently bound after MBC periodate activation was very stable during operational stability testing; it could be repeatedly used for ten cycles of low molecular weight substrate hydrolysis without loss of its initial activity.
Department of Chemical Engineering University of Patras 26504 Patras Rio Greece
Global Change Research Institute CAS Na Sadkach 7 370 05 Ceske Budejovice Czech Republic
Citace poskytuje Crossref.org
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- $a Bacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was magnetically modified using perchloric acid stabilized magnetic fluid. Magnetic bacterial cellulose (MBC) was used as a carrier for the immobilization of affinity ligands, enzymes and cells. MBC with immobilized reactive copper phthalocyanine dye was an efficient adsorbent for crystal violet removal; the maximum adsorption capacity was 388mg/g. Kinetic and thermodynamic parameters were also determined. Model biocatalysts, namely bovine pancreas trypsin and Saccharomyces cerevisiae cells were immobilized on MBC using several strategies including adsorption with subsequent cross-linking with glutaraldehyde and covalent binding on previously activated MBC using sodium periodate or 1,4-butanediol diglycidyl ether. Immobilized yeast cells retained approximately 90% of their initial activity after 6 repeated cycles of sucrose solution hydrolysis. Trypsin covalently bound after MBC periodate activation was very stable during operational stability testing; it could be repeatedly used for ten cycles of low molecular weight substrate hydrolysis without loss of its initial activity.
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