• Je něco špatně v tomto záznamu ?

The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy

V. Milosavljevic, Y. Haddad, MA. Merlos Rodrigo, A. Moulick, H. Polanska, D. Hynek, Z. Heger, P. Kopel, V. Adam,

. 2016 ; 11 (10) : e0163983. [pub] 20161011

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17023644

Prostate cancer cells control energy metabolism by chelating intracellular zinc. Thus, zinc delivery has been a popular therapeutic approach for prostate cancer. Here, we propose the use of the membrane-penetrating peptide Novicidin connected to zinc-Schiff base as a carrier vehicle for the delivery of zinc to prostate cells. Mass spectrometry, electrochemistry and spectrophotometry confirmed the formation/stability of this complex and provided insight regarding the availability of zinc for complex interactions. This delivery system showed minor toxicity in normal PNT1A cells and high potency towards PC3 tumor cells. The complex preferentially penetrated PC3 tumor cells in contrast to confinement to the membranes of PNT1A. Furthermore, zinc uptake was confirmed in both cell lines. Molecular analysis was used to confirm the activation of zinc stress (e.g., ZnT-1) and apoptosis (e.g., CASP-1). Our results strongly suggest that the zinc-Schiff base-Novicidin complex has great potential as a novel anticancer drug.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17023644
003      
CZ-PrNML
005      
20170720122309.0
007      
ta
008      
170720s2016 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.pone.0163983 $2 doi
035    __
$a (PubMed)27727290
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Milosavljevic, Vedran $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic, European Union.
245    14
$a The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy / $c V. Milosavljevic, Y. Haddad, MA. Merlos Rodrigo, A. Moulick, H. Polanska, D. Hynek, Z. Heger, P. Kopel, V. Adam,
520    9_
$a Prostate cancer cells control energy metabolism by chelating intracellular zinc. Thus, zinc delivery has been a popular therapeutic approach for prostate cancer. Here, we propose the use of the membrane-penetrating peptide Novicidin connected to zinc-Schiff base as a carrier vehicle for the delivery of zinc to prostate cells. Mass spectrometry, electrochemistry and spectrophotometry confirmed the formation/stability of this complex and provided insight regarding the availability of zinc for complex interactions. This delivery system showed minor toxicity in normal PNT1A cells and high potency towards PC3 tumor cells. The complex preferentially penetrated PC3 tumor cells in contrast to confinement to the membranes of PNT1A. Furthermore, zinc uptake was confirmed in both cell lines. Molecular analysis was used to confirm the activation of zinc stress (e.g., ZnT-1) and apoptosis (e.g., CASP-1). Our results strongly suggest that the zinc-Schiff base-Novicidin complex has great potential as a novel anticancer drug.
650    _2
$a kationické antimikrobiální peptidy $x chemie $7 D023181
650    _2
$a antitumorózní látky $x chemie $x farmakologie $x terapeutické užití $7 D000970
650    _2
$a apoptóza $x účinky léků $7 D017209
650    _2
$a kaspasa 1 $x metabolismus $7 D020170
650    _2
$a proteiny přenášející kationty $x metabolismus $7 D027682
650    _2
$a nádorové buněčné linie $7 D045744
650    _2
$a komplexní sloučeniny $x chemie $x farmakologie $x terapeutické užití $7 D056831
650    _2
$a exprese genu $x účinky léků $7 D015870
650    _2
$a lidé $7 D006801
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a fluorescenční mikroskopie $7 D008856
650    _2
$a molekulární konformace $7 D008968
650    _2
$a nádory prostaty $x farmakoterapie $7 D011471
650    _2
$a Schiffovy báze $x chemie $7 D012545
650    _2
$a spektrometrie hmotnostní - ionizace laserem za účasti matrice $7 D019032
650    _2
$a zinek $x chemie $x metabolismus $7 D015032
655    _2
$a časopisecké články $7 D016428
700    1_
$a Haddad, Yazan $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic, European Union.
700    1_
$a Merlos Rodrigo, Miguel Angel $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic, European Union. Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00, Brno, Czech Republic, European Union.
700    1_
$a Moulick, Amitava $u Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00, Brno, Czech Republic, European Union.
700    1_
$a Polanska, Hana $u Department of Physiology, Faculty of Medicine, Masaryk University, Kamenice 5, CZ-625 00, Brno, Czech Republic, European Union.
700    1_
$a Hynek, David $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic, European Union. Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00, Brno, Czech Republic, European Union.
700    1_
$a Heger, Zbynek $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic, European Union. Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00, Brno, Czech Republic, European Union.
700    1_
$a Kopel, Pavel $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic, European Union. Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00, Brno, Czech Republic, European Union.
700    1_
$a Adam, Vojtěch $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00, Brno, Czech Republic, European Union. Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00, Brno, Czech Republic, European Union. $7 xx0064599
773    0_
$w MED00180950 $t PloS one $x 1932-6203 $g Roč. 11, č. 10 (2016), s. e0163983
856    41
$u https://pubmed.ncbi.nlm.nih.gov/27727290 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20170720 $b ABA008
991    __
$a 20170720122802 $b ABA008
999    __
$a ok $b bmc $g 1239325 $s 984557
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 11 $c 10 $d e0163983 $e 20161011 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
LZP    __
$a Pubmed-20170720

Najít záznam

Citační ukazatele

Možnosti archivace