Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Esc2 promotes Mus81 complex-activity via its SUMO-like and DNA binding domains

M. Sebesta, M. Urulangodi, B. Stefanovie, B. Szakal, M. Pacesa, M. Lisby, D. Branzei, L. Krejci,

. 2017 ; 45 (1) : 215-230. [pub] 20160930

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17023660

Replication across damaged DNA templates is accompanied by transient formation of sister chromatid junctions (SCJs). Cells lacking Esc2, an adaptor protein containing no known enzymatic domains, are defective in the metabolism of these SCJs. However, how Esc2 is involved in the metabolism of SCJs remains elusive. Here we show interaction between Esc2 and a structure-specific endonuclease Mus81-Mms4 (the Mus81 complex), their involvement in the metabolism of SCJs, and the effects Esc2 has on the enzymatic activity of the Mus81 complex. We found that Esc2 specifically interacts with the Mus81 complex via its SUMO-like domains, stimulates enzymatic activity of the Mus81 complex in vitro, and is involved in the Mus81 complex-dependent resolution of SCJs in vivo Collectively, our data point to the possibility that the involvement of Esc2 in the metabolism of SCJs is, in part, via modulation of the activity of the Mus81 complex.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17023660
003      
CZ-PrNML
005      
20170720123542.0
007      
ta
008      
170720s2017 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/nar/gkw882 $2 doi
035    __
$a (PubMed)27694623
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Sebesta, Marek $u National Centre for Biomolecular Research, Masaryk University, Kamenice 5/A4, CZ-62500 Brno, Czech Republic. Department of Biology, Masaryk University, Kamenice 5/A7, CZ-62500 Brno, Czech Republic. IFOM, the FIRC Institute of Molecular Oncology, Via Adamello 16, IT-20139 Milan, Italy.
245    10
$a Esc2 promotes Mus81 complex-activity via its SUMO-like and DNA binding domains / $c M. Sebesta, M. Urulangodi, B. Stefanovie, B. Szakal, M. Pacesa, M. Lisby, D. Branzei, L. Krejci,
520    9_
$a Replication across damaged DNA templates is accompanied by transient formation of sister chromatid junctions (SCJs). Cells lacking Esc2, an adaptor protein containing no known enzymatic domains, are defective in the metabolism of these SCJs. However, how Esc2 is involved in the metabolism of SCJs remains elusive. Here we show interaction between Esc2 and a structure-specific endonuclease Mus81-Mms4 (the Mus81 complex), their involvement in the metabolism of SCJs, and the effects Esc2 has on the enzymatic activity of the Mus81 complex. We found that Esc2 specifically interacts with the Mus81 complex via its SUMO-like domains, stimulates enzymatic activity of the Mus81 complex in vitro, and is involved in the Mus81 complex-dependent resolution of SCJs in vivo Collectively, our data point to the possibility that the involvement of Esc2 in the metabolism of SCJs is, in part, via modulation of the activity of the Mus81 complex.
650    _2
$a chromatidy $x chemie $x metabolismus $7 D002842
650    _2
$a klonování DNA $7 D003001
650    _2
$a poškození DNA $7 D004249
650    _2
$a replikace DNA $7 D004261
650    _2
$a křížová struktura DNA $x chemie $x metabolismus $7 D045566
650    _2
$a DNA fungální $x genetika $x metabolismus $7 D004271
650    _2
$a DNA vazebné proteiny $x chemie $x genetika $x metabolismus $7 D004268
650    _2
$a endonukleasy $x chemie $x genetika $x metabolismus $7 D004720
650    _2
$a Escherichia coli $x genetika $x metabolismus $7 D004926
650    12
$a regulace genové exprese u hub $7 D015966
650    _2
$a nestabilita genomu $7 D042822
650    _2
$a jaderné proteiny $x chemie $x genetika $x metabolismus $7 D009687
650    _2
$a vazba proteinů $7 D011485
650    _2
$a proteinové domény $7 D000072417
650    _2
$a rekombinantní proteiny $x chemie $x genetika $x metabolismus $7 D011994
650    _2
$a Saccharomyces cerevisiae $x genetika $x metabolismus $7 D012441
650    _2
$a Saccharomyces cerevisiae - proteiny $x chemie $x genetika $x metabolismus $7 D029701
650    _2
$a malé modifikační proteiny související s ubikvitinem $x chemie $x genetika $x metabolismus $7 D025841
655    _2
$a časopisecké články $7 D016428
700    1_
$a Urulangodi, Madhusoodanan $u IFOM, the FIRC Institute of Molecular Oncology, Via Adamello 16, IT-20139 Milan, Italy.
700    1_
$a Stefanovie, Barbora $u National Centre for Biomolecular Research, Masaryk University, Kamenice 5/A4, CZ-62500 Brno, Czech Republic. Department of Biology, Masaryk University, Kamenice 5/A7, CZ-62500 Brno, Czech Republic. International Clinical Research Center, Center for Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Pekarska 53, CZ-656 91 Brno, Czech Republic.
700    1_
$a Szakal, Barnabas $u IFOM, the FIRC Institute of Molecular Oncology, Via Adamello 16, IT-20139 Milan, Italy.
700    1_
$a Pacesa, Martin $u National Centre for Biomolecular Research, Masaryk University, Kamenice 5/A4, CZ-62500 Brno, Czech Republic.
700    1_
$a Lisby, Michael $u Department of Biology, University of Copenhagen, DK-2200 Copenhagen, Denmark.
700    1_
$a Branzei, Dana $u IFOM, the FIRC Institute of Molecular Oncology, Via Adamello 16, IT-20139 Milan, Italy.
700    1_
$a Krejci, Lumir $u National Centre for Biomolecular Research, Masaryk University, Kamenice 5/A4, CZ-62500 Brno, Czech Republic lkrejci@chemi.muni.cz. Department of Biology, Masaryk University, Kamenice 5/A7, CZ-62500 Brno, Czech Republic. International Clinical Research Center, Center for Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Pekarska 53, CZ-656 91 Brno, Czech Republic.
773    0_
$w MED00003554 $t Nucleic acids research $x 1362-4962 $g Roč. 45, č. 1 (2017), s. 215-230
856    41
$u https://pubmed.ncbi.nlm.nih.gov/27694623 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20170720 $b ABA008
991    __
$a 20170720124035 $b ABA008
999    __
$a ok $b bmc $g 1239341 $s 984573
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 45 $c 1 $d 215-230 $e 20160930 $i 1362-4962 $m Nucleic acids research $n Nucleic Acids Res $x MED00003554
LZP    __
$a Pubmed-20170720

Najít záznam

Citační ukazatele

Možnosti archivace