BACKGROUND: Adhesion molecules are involved in the development of atherosclerosis. An increased level of the ICAM 1 molecule is associated with numerous inflammatory diseases including atherosclerosis of carotid arteries. The rs5498 (K469E) polymorphism of the ICAM-1 gene leads to an increase in the level of serum ICAM. We investigated the association between the rs5498 (K469E) polymorphism of the ICAM-1 gene and the progression of carotid atherosclerosis in subjects with type 2 diabetes mellitus (T2DM). METHODS: The study included 595 patients with T2DM and 200 subjects in the control group without T2DM. The control examination was made 3.8 years after the initial examination. Indicators of atherosclerosis (carotid intima-media thickness (CIMT), total plaque sum and sum of the plaques thickness) were detected by ultrasound examination. Genetic analyses of the polymorphism rs5498 of the ICAM-1 gene were made by RT-PCR. RESULTS: The distribution of genotypes and frequencies of rs5498 polymorphism was not significantly different between the group with type 2 diabetes ( T2DM) and the control group. Genotype EE K469E polymorphism is associated with a statistically significant annual plaques growth. CONCLUSION: The EE genotype of the rs5498 of the ICAM-1 gene was associated with a more rapid progression of carotid atherosclerosis in patients with T2DM in comparison with other genotypes.

2nd Department of Internal Medicine Faculty of Medicine Comenius University and University Hospital Bratislava Slovakia

General Hospital Rakičan Murska Sobota Slovenia

General Hospital Slovenj Gradec Slovenj Gradec Slovenia

Institute of Histology and Embryology Faculty of Medicine University of Ljubljana Ljubljana Slovenia

Laboratory of Structural Biology and Proteomics Faculty of Pharmacy University of Veterinary and Pharmaceutical Sciences Palackeho tr 1946 1 612 42 Brno Czech Republic

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$a Polymorphism rs5498 of the ICAM-1 gene affects the progression of carotid atherosclerosis in patients with type 2 diabetes mellitus / $c D. Popović, JN. Starčević, MŠ. Letonja, J. Makuc, AC. Vujkovac, RZ. Pleskovič, L. Gaspar, P. Kruzliak, D. Petrovič,

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$a BACKGROUND: Adhesion molecules are involved in the development of atherosclerosis. An increased level of the ICAM 1 molecule is associated with numerous inflammatory diseases including atherosclerosis of carotid arteries. The rs5498 (K469E) polymorphism of the ICAM-1 gene leads to an increase in the level of serum ICAM. We investigated the association between the rs5498 (K469E) polymorphism of the ICAM-1 gene and the progression of carotid atherosclerosis in subjects with type 2 diabetes mellitus (T2DM). METHODS: The study included 595 patients with T2DM and 200 subjects in the control group without T2DM. The control examination was made 3.8 years after the initial examination. Indicators of atherosclerosis (carotid intima-media thickness (CIMT), total plaque sum and sum of the plaques thickness) were detected by ultrasound examination. Genetic analyses of the polymorphism rs5498 of the ICAM-1 gene were made by RT-PCR. RESULTS: The distribution of genotypes and frequencies of rs5498 polymorphism was not significantly different between the group with type 2 diabetes ( T2DM) and the control group. Genotype EE K469E polymorphism is associated with a statistically significant annual plaques growth. CONCLUSION: The EE genotype of the rs5498 of the ICAM-1 gene was associated with a more rapid progression of carotid atherosclerosis in patients with T2DM in comparison with other genotypes.

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$a Starčević, Jovana Nikolajević $u Institute of Histology and Embryology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

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$a Letonja, Marija Šantl $u General Hospital Rakičan, Murska Sobota, Slovenia.

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$a Makuc, Jana $u General Hospital Slovenj Gradec, Slovenj Gradec, Slovenia.

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$a Vujkovac, Andreja Cokan $u General Hospital Slovenj Gradec, Slovenj Gradec, Slovenia.

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$a Pleskovič, Ruda Zorc $u Institute of Histology and Embryology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

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$a Gaspar, Ludovit $u 2nd Department of Internal Medicine, Faculty of Medicine, Comenius University and University Hospital, Bratislava, Slovakia. ludovitgaspar@gmail.com.

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$a Kruzliak, Peter $u Laboratory of Structural Biology and Proteomics, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences, Palackeho tr. 1946/1, 612 42, Brno, Czech Republic. peter.kruzliak@savba.sk.

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$a Petrovič, Danijel $u Institute of Histology and Embryology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia. Daniel.petrovic@mf.uni-lj.si.

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