-
Je něco špatně v tomto záznamu ?
Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold
A. Norek, L. Janda,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
NLK
Free Medical Journals
od 1992 do Před 1 rokem
PubMed Central
od 1992 do Před 1 rokem
Europe PubMed Central
od 1992 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2010-01-01 do Před 1 rokem
Wiley Free Content
od 1996 do Před 1 rokem
PubMed
28142214
DOI
10.1002/pro.3125
Knihovny.cz E-zdroje
- MeSH
- antigeny bakteriální chemie genetika imunologie MeSH
- Borrelia burgdorferi chemie genetika imunologie MeSH
- mapování epitopu * MeSH
- multimerizace proteinu * MeSH
- myši inbrední BALB C MeSH
- myší monoklonální protilátky chemie imunologie MeSH
- myši MeSH
- proteiny vnější bakteriální membrány chemie genetika imunologie MeSH
- sbalování proteinů MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
In current work, we used recombinant OspC protein derived from B. afzelii strain BRZ31 in the native homodimeric fold for mice immunization and following selection process to produce three mouse monoclonal antibodies able to bind to variable parts of up to five different OspC proteins. Applying the combination of mass spectrometry assisted epitope mapping and affinity based theoretical prediction we have localized regions responsible for antigen-antibody interactions and approximate epitopes' amino acid composition. Two mAbs (3F4 and 2A9) binds to linear epitopes located in previously described immunogenic regions in the exposed part of OspC protein. The third mAb (2D1) recognises highly conserved discontinuous epitope close to the ligand binding domain 1.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17031078
- 003
- CZ-PrNML
- 005
- 20171101114930.0
- 007
- ta
- 008
- 171025s2017 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1002/pro.3125 $2 doi
- 035 __
- $a (PubMed)28142214
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Norek, Adam $u CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 753/5, Brno, 62500, Czech Republic. Department of Animal Physiology and Immunology, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 753/5, Brno, 62500, Czech Republic.
- 245 10
- $a Epitope mapping of Borrelia burgdorferi OspC protein in homodimeric fold / $c A. Norek, L. Janda,
- 520 9_
- $a In current work, we used recombinant OspC protein derived from B. afzelii strain BRZ31 in the native homodimeric fold for mice immunization and following selection process to produce three mouse monoclonal antibodies able to bind to variable parts of up to five different OspC proteins. Applying the combination of mass spectrometry assisted epitope mapping and affinity based theoretical prediction we have localized regions responsible for antigen-antibody interactions and approximate epitopes' amino acid composition. Two mAbs (3F4 and 2A9) binds to linear epitopes located in previously described immunogenic regions in the exposed part of OspC protein. The third mAb (2D1) recognises highly conserved discontinuous epitope close to the ligand binding domain 1.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a myší monoklonální protilátky $x chemie $x imunologie $7 D058846
- 650 _2
- $a antigeny bakteriální $x chemie $x genetika $x imunologie $7 D000942
- 650 _2
- $a proteiny vnější bakteriální membrány $x chemie $x genetika $x imunologie $7 D001425
- 650 _2
- $a Borrelia burgdorferi $x chemie $x genetika $x imunologie $7 D025065
- 650 12
- $a mapování epitopu $7 D018604
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a myši $7 D051379
- 650 _2
- $a myši inbrední BALB C $7 D008807
- 650 _2
- $a sbalování proteinů $7 D017510
- 650 12
- $a multimerizace proteinu $7 D055503
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Janda, Lubomír $u CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 753/5, Brno, 62500, Czech Republic.
- 773 0_
- $w MED00008270 $t Protein science a publication of the Protein Society $x 1469-896X $g Roč. 26, č. 4 (2017), s. 796-806
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/28142214 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20171025 $b ABA008
- 991 __
- $a 20171101115021 $b ABA008
- 999 __
- $a ok $b bmc $g 1254671 $s 992105
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 26 $c 4 $d 796-806 $e 20170211 $i 1469-896X $m Protein science $n Protein Sci $x MED00008270
- LZP __
- $a Pubmed-20171025
