Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Increased Number of Mast Cells in Epicardial Adipose Tissue of Cardiac Surgery Patients With Coronary Artery Disease

K. Rozsívalová, A. Pierzynová, H. Kratochvílová, J. Lindner, M. Lipš, T. Kotulák, P. Ivák, I. Netuka, M. Haluzík, T. Kučera

. 2020 ; 69 (4) : 621-631. [pub] 20200625

Language English Country Czech Republic

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc21021224

Chronic inflammation of adipose tissue is associated with the pathogenesis of cardiovascular diseases. Mast cells represent an important component of the innate defense system of the organism. In our work, we quantified mast cell number in epicardial adipose tissue (EAT), subcutaneous adipose tissue (SAT), and right atrial myocardium (RA) in patients undergoing open heart surgery (n=57). Bioptic samples of EAT (n=44), SAT (n=42) and RA (n=17) were fixed by 4 % paraformaldehyde and embedded into paraffin. An anti-mast cell tryptase antibody was used for immunohistochemical detection and quantification of mast cells. We also demonstrated immunohistochemically the expression of CD117 and chymase markers. In EAT of patients with coronary artery disease (CAD), higher incidence of mast cells has been found compared to patients without CAD (3.7±2.6 vs. 2.1±1.2 cells/mm(2)). In SAT and RA, there was no difference in the number of mast cells in CAD and non-CAD patients. Mast cells in SAT, EAT and RA expressed CD117 and chymase. An increased incidence of mast cells in EAT of CAD patients may indicate the specific role of these inflammatory cells in relation to EAT and coronary arteries affected by atherosclerosis.

References provided by Crossref.org

Bibliography, etc.

Literatura

000      
00000naa a2200000 a 4500
001      
bmc21021224
003      
CZ-PrNML
005      
20210920095419.0
007      
ta
008      
210806s2020 xr d f 000 0|eng||
009      
AR
024    7_
$a 10.33549/physiolres.934344 $2 doi
035    __
$a (PubMed)32584133
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xr
100    1_
$a Rozsívalová, Karolína $7 xx0264156 $u Institute of Histology and Embryology, First Faculty of Medicine, Charles University, Prague, Czech Republic
245    10
$a Increased Number of Mast Cells in Epicardial Adipose Tissue of Cardiac Surgery Patients With Coronary Artery Disease / $c K. Rozsívalová, A. Pierzynová, H. Kratochvílová, J. Lindner, M. Lipš, T. Kotulák, P. Ivák, I. Netuka, M. Haluzík, T. Kučera
504    __
$a Literatura
520    9_
$a Chronic inflammation of adipose tissue is associated with the pathogenesis of cardiovascular diseases. Mast cells represent an important component of the innate defense system of the organism. In our work, we quantified mast cell number in epicardial adipose tissue (EAT), subcutaneous adipose tissue (SAT), and right atrial myocardium (RA) in patients undergoing open heart surgery (n=57). Bioptic samples of EAT (n=44), SAT (n=42) and RA (n=17) were fixed by 4 % paraformaldehyde and embedded into paraffin. An anti-mast cell tryptase antibody was used for immunohistochemical detection and quantification of mast cells. We also demonstrated immunohistochemically the expression of CD117 and chymase markers. In EAT of patients with coronary artery disease (CAD), higher incidence of mast cells has been found compared to patients without CAD (3.7±2.6 vs. 2.1±1.2 cells/mm(2)). In SAT and RA, there was no difference in the number of mast cells in CAD and non-CAD patients. Mast cells in SAT, EAT and RA expressed CD117 and chymase. An increased incidence of mast cells in EAT of CAD patients may indicate the specific role of these inflammatory cells in relation to EAT and coronary arteries affected by atherosclerosis.
650    _2
$a tuková tkáň $x metabolismus $x patologie $7 D000273
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    _2
$a senioři nad 80 let $7 D000369
650    _2
$a biologické markery $x metabolismus $7 D015415
650    _2
$a kardiochirurgické výkony $x škodlivé účinky $x metody $7 D006348
650    _2
$a nemoci koronárních tepen $x metabolismus $x patologie $7 D003324
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a lidé $7 D006801
650    _2
$a zánět $x etiologie $x metabolismus $x patologie $7 D007249
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a mastocyty $x metabolismus $x patologie $7 D008407
650    _2
$a lidé středního věku $7 D008875
650    _2
$a myokard $x metabolismus $x patologie $7 D009206
650    _2
$a perikard $x metabolismus $x patologie $7 D010496
655    _2
$a časopisecké články $7 D016428
700    1_
$a Pierzynová, Aneta $7 xx0224306 $u Institute of Histology and Embryology, First Faculty of Medicine, Charles University, Prague, Czech Republic
700    1_
$a Kratochvílová, Helena, $d 1992- $7 xx0228161 $u Institute of Medical Biochemistry & Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic $u Centre for Experimental Medicine and Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
700    1_
$a Lindner, Jaroslav, $d 1957- $7 mzk2005269705 $u Second Department of Surgery – Department of Cardiovascular Surgery, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic
700    1_
$a Lipš, Michal $7 xx0232731 $u Department of Anesthesiology, Resuscitation and Intensive Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic
700    1_
$a Kotulák, Tomáš $7 xx0108024 $u Department of Anesthesiology, Resuscitation and Intensive Medicine, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic
700    1_
$a Ivák, Peter $7 jo2012699691 $u Department of Cardiovascular Surgery, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
700    1_
$a Netuka, Ivan, $d 1973- $7 xx0074183 $u Department of Cardiovascular Surgery, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
700    1_
$a Haluzík, Martin, $d 1970- $7 xx0000707 $u Institute of Medical Biochemistry & Laboratory Diagnostics, First Faculty of Medicine, Charles University and General University Hospital, Prague, Czech Republic $u Centre for Experimental Medicine and Diabetes Centre, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
700    1_
$a Kučera, Tomáš $7 xx0086044 $u Institute of Histology and Embryology, First Faculty of Medicine, Charles University, Prague, Czech Republic
773    0_
$w MED00003824 $t Physiological research $x 1802-9973 $g Roč. 69, č. 4 (2020), s. 621-631
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32584133 $y Pubmed
910    __
$a ABA008 $b A 4120 $c 266 $y p $z 0
990    __
$a 20210806 $b ABA008
991    __
$a 20210920095419 $b ABA008
999    __
$a ok $b bmc $g 1697029 $s 1141677
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 69 $c 4 $d 621-631 $e 20200625 $i 1802-9973 $m Physiological research $n Physiol. Res. (Print) $x MED00003824
LZP    __
$b NLK118 $a Pubmed-20210806

Find record

Citation metrics

Archiving options