• Je něco špatně v tomto záznamu ?

Flow Analysis and Sorting of Plant Chromosomes

J. Vrána, P. Cápal, H. Šimková, M. Karafiátová, J. Čížková, J. Doležel,

. 2016 ; 78 (-) : 5.3.1-5.3.43. [pub] 20161010

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc17031396

Analysis and sorting of plant chromosomes (plant flow cytogenetics) is a special application of flow cytometry in plant genomics and its success depends critically on sample quality. This unit describes the methodology in a stepwise manner, starting with the induction of cell cycle synchrony and accumulation of dividing cells in mitotic metaphase, and continues with the preparation of suspensions of intact mitotic chromosomes, flow analysis and sorting of chromosomes, and finally processing of the sorted chromosomes. Each step of the protocol is described in detail as some procedures have not been used widely. Supporting histograms are presented as well as hints on dealing with plant material; the utility of sorted chromosomes for plant genomics is also discussed. © 2016 by John Wiley & Sons, Inc.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc17031396
003      
CZ-PrNML
005      
20171127090257.0
007      
ta
008      
171025s2016 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1002/cpcy.9 $2 doi
035    __
$a (PubMed)27723090
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Vrána, Jan $u Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
245    10
$a Flow Analysis and Sorting of Plant Chromosomes / $c J. Vrána, P. Cápal, H. Šimková, M. Karafiátová, J. Čížková, J. Doležel,
520    9_
$a Analysis and sorting of plant chromosomes (plant flow cytogenetics) is a special application of flow cytometry in plant genomics and its success depends critically on sample quality. This unit describes the methodology in a stepwise manner, starting with the induction of cell cycle synchrony and accumulation of dividing cells in mitotic metaphase, and continues with the preparation of suspensions of intact mitotic chromosomes, flow analysis and sorting of chromosomes, and finally processing of the sorted chromosomes. Each step of the protocol is described in detail as some procedures have not been used widely. Supporting histograms are presented as well as hints on dealing with plant material; the utility of sorted chromosomes for plant genomics is also discussed. © 2016 by John Wiley & Sons, Inc.
650    _2
$a chromozomy rostlin $x metabolismus $7 D032461
650    _2
$a DNA rostlinná $x genetika $7 D018744
650    _2
$a průtoková cytometrie $x metody $7 D005434
650    _2
$a hybridizace in situ fluorescenční $7 D017404
650    _2
$a karyotypizace $7 D007621
650    _2
$a meristém $x cytologie $x účinky léků $7 D018519
650    _2
$a metafáze $x účinky léků $7 D008677
650    _2
$a molekulová hmotnost $7 D008970
650    _2
$a oxid dusný $x farmakologie $7 D009609
650    _2
$a rostliny $x genetika $7 D010944
650    _2
$a proteomika $7 D040901
650    _2
$a semena rostlinná $x účinky léků $7 D012639
655    _2
$a časopisecké články $7 D016428
700    1_
$a Cápal, Petr $u Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
700    1_
$a Šimková, Hana $u Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
700    1_
$a Karafiátová, Miroslava $u Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
700    1_
$a Čížková, Jana $u Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
700    1_
$a Doležel, Jaroslav $u Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
773    0_
$w MED00193506 $t Current protocols in cytometry $x 1934-9300 $g Roč. 78, č. - (2016), s. 5.3.1-5.3.43
856    41
$u https://pubmed.ncbi.nlm.nih.gov/27723090 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20171025 $b ABA008
991    __
$a 20171127090423 $b ABA008
999    __
$a ok $b bmc $g 1254989 $s 992423
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2016 $b 78 $c - $d 5.3.1-5.3.43 $e 20161010 $i 1934-9300 $m Current protocols in cytometry $n Curr Protoc Cytom $x MED00193506
LZP    __
$a Pubmed-20171025

Najít záznam

Citační ukazatele

Možnosti archivace