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Prevalence of Propionibacterium acnes in Intervertebral Discs of Patients Undergoing Lumbar Microdiscectomy: A Prospective Cross-Sectional Study
MN. Capoor, F. Ruzicka, T. Machackova, R. Jancalek, M. Smrcka, JE. Schmitz, M. Hermanova, J. Sana, E. Michu, JC. Baird, FS. Ahmed, K. Maca, R. Lipina, TF. Alamin, MF. Coscia, JL. Stonemetz, T. Witham, GD. Ehrlich, ZL. Gokaslan, K. Mavrommatis, C....
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
NLK
Directory of Open Access Journals
od 2006
Free Medical Journals
od 2006
Public Library of Science (PLoS)
od 2006
PubMed Central
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ProQuest Central
od 2006-12-01
Open Access Digital Library
od 2006-01-01
Open Access Digital Library
od 2006-10-01
Open Access Digital Library
od 2006-01-01
Medline Complete (EBSCOhost)
od 2008-01-01
Nursing & Allied Health Database (ProQuest)
od 2006-12-01
Health & Medicine (ProQuest)
od 2006-12-01
Public Health Database (ProQuest)
od 2006-12-01
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od 2006
- MeSH
- bederní obratle chirurgie MeSH
- degenerace meziobratlové ploténky komplikace mikrobiologie chirurgie MeSH
- diskektomie metody statistika a číselné údaje MeSH
- dospělí MeSH
- grampozitivní bakteriální infekce komplikace epidemiologie mikrobiologie MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé středního věku MeSH
- lidé MeSH
- meziobratlová ploténka mikrobiologie chirurgie MeSH
- prevalence MeSH
- Propionibacterium acnes * MeSH
- prospektivní studie MeSH
- průřezové studie MeSH
- rizikové faktory MeSH
- věkové faktory MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: The relationship between intervertebral disc degeneration and chronic infection by Propionibacterium acnes is controversial with contradictory evidence available in the literature. Previous studies investigating these relationships were under-powered and fraught with methodical differences; moreover, they have not taken into consideration P. acnes' ability to form biofilms or attempted to quantitate the bioburden with regard to determining bacterial counts/genome equivalents as criteria to differentiate true infection from contamination. The aim of this prospective cross-sectional study was to determine the prevalence of P. acnes in patients undergoing lumbar disc microdiscectomy. METHODS AND FINDINGS: The sample consisted of 290 adult patients undergoing lumbar microdiscectomy for symptomatic lumbar disc herniation. An intraoperative biopsy and pre-operative clinical data were taken in all cases. One biopsy fragment was homogenized and used for quantitative anaerobic culture and a second was frozen and used for real-time PCR-based quantification of P. acnes genomes. P. acnes was identified in 115 cases (40%), coagulase-negative staphylococci in 31 cases (11%) and alpha-hemolytic streptococci in 8 cases (3%). P. acnes counts ranged from 100 to 9000 CFU/ml with a median of 400 CFU/ml. The prevalence of intervertebral discs with abundant P. acnes (≥ 1x103 CFU/ml) was 11% (39 cases). There was significant correlation between the bacterial counts obtained by culture and the number of P. acnes genomes detected by real-time PCR (r = 0.4363, p<0.0001). CONCLUSIONS: In a large series of patients, the prevalence of discs with abundant P. acnes was 11%. We believe, disc tissue homogenization releases P. acnes from the biofilm so that they can then potentially be cultured, reducing the rate of false-negative cultures. Further, quantification study revealing significant bioburden based on both culture and real-time PCR minimize the likelihood that observed findings are due to contamination and supports the hypothesis P. acnes acts as a pathogen in these cases of degenerative disc disease.
Celgene Corporation Information Knowledge and Utilization San Francisco CA United States of America
Department of Anesthesia The Johns Hopkins Hospital Baltimore MD United States of America
Department of Neurosurgery St Anne's University Hospital Masaryk University Brno Czech Republic
Department of Neurosurgery The Johns Hopkins Hospital Baltimore MD United States of America
Department of Neurosurgery University Hospital Brno Masaryk University Brno Czech Republic
Department of Neurosurgery University Hospital Ostrava Ostrava University Ostrava Czech Republic
Department of Orthopedic Surgery OrthoIndy Hospital Indianapolis IN United States of America
Department of Orthopedics Physical Medicine and Rehabilitation University of Munich Munich Germany
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- $a Capoor, Manu N $u Laboratory of Bacterial Pathogenesis and Immunology, Rockefeller University, New York, NY, United States of America. Department of Molecular Oncology, Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic.
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- $a Prevalence of Propionibacterium acnes in Intervertebral Discs of Patients Undergoing Lumbar Microdiscectomy: A Prospective Cross-Sectional Study / $c MN. Capoor, F. Ruzicka, T. Machackova, R. Jancalek, M. Smrcka, JE. Schmitz, M. Hermanova, J. Sana, E. Michu, JC. Baird, FS. Ahmed, K. Maca, R. Lipina, TF. Alamin, MF. Coscia, JL. Stonemetz, T. Witham, GD. Ehrlich, ZL. Gokaslan, K. Mavrommatis, C. Birkenmaier, VA. Fischetti, O. Slaby,
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