-
Je něco špatně v tomto záznamu ?
Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen
C. Machacek, V. Supper, V. Leksa, G. Mitulovic, A. Spittler, K. Drbal, M. Suchanek, A. Ohradanova-Repic, H. Stockinger,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
NLK
Free Medical Journals
od 1998 do Před 1 rokem
Freely Accessible Science Journals
od 1998-01-01 do Před 1 rokem
Open Access Digital Library
od 1998-01-01
PubMed
27534550
DOI
10.4049/jimmunol.1501878
Knihovny.cz E-zdroje
- MeSH
- antigeny CD11b fyziologie MeSH
- antigeny CD18 fyziologie MeSH
- buněčná adheze MeSH
- folátový receptor 2 fyziologie MeSH
- kolagen farmakologie MeSH
- kultivované buňky MeSH
- kyselina listová metabolismus MeSH
- lidé MeSH
- makrofágy fyziologie MeSH
- pohyb buněk MeSH
- proliferace buněk MeSH
- tetradekanoylforbolacetát farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Folate, also known as vitamin B9, is necessary for essential cellular functions such as DNA synthesis, repair, and methylation. It is supplied to the cell via several transporters and receptors, including folate receptor (FR) β, a GPI-anchored protein belonging to the folate receptor family. As FRβ shows a restricted expression to cells of myeloid origin and only a subset of activated macrophages and placental cells have been shown to express functional FRβ, it represents a promising target for future therapeutic strategies. In this study, we performed affinity purification and mass spectrometric analysis of the protein microenvironment of FRβ in the plasma membrane of human FRβ(+) macrophages and FRβ-transduced monocytic THP-1 cells. In this manner, we identified a novel role of FRβ: that is, we report functional interactions of FRβ with receptors mediating cellular adhesion, in particular the CD11b/CD18 β2 integrin heterodimer complement receptor type 3/Mac-1. This interaction results in impeded adhesion of FRβ(+) human primary macrophages and THP-1 cells to collagen in comparison with their FRβ(-) counterparts. We further show that FRβ is only expressed by human macrophages when differentiated with M-CSF. These findings thus identify FRβ as a novel CD11b/CD18 regulator for trafficking and homing of a subset of macrophages on collagen.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc17031505
- 003
- CZ-PrNML
- 005
- 20171025123028.0
- 007
- ta
- 008
- 171025s2016 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.4049/jimmunol.1501878 $2 doi
- 035 __
- $a (PubMed)27534550
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Machacek, Christian $u Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria;
- 245 10
- $a Folate Receptor β Regulates Integrin CD11b/CD18 Adhesion of a Macrophage Subset to Collagen / $c C. Machacek, V. Supper, V. Leksa, G. Mitulovic, A. Spittler, K. Drbal, M. Suchanek, A. Ohradanova-Repic, H. Stockinger,
- 520 9_
- $a Folate, also known as vitamin B9, is necessary for essential cellular functions such as DNA synthesis, repair, and methylation. It is supplied to the cell via several transporters and receptors, including folate receptor (FR) β, a GPI-anchored protein belonging to the folate receptor family. As FRβ shows a restricted expression to cells of myeloid origin and only a subset of activated macrophages and placental cells have been shown to express functional FRβ, it represents a promising target for future therapeutic strategies. In this study, we performed affinity purification and mass spectrometric analysis of the protein microenvironment of FRβ in the plasma membrane of human FRβ(+) macrophages and FRβ-transduced monocytic THP-1 cells. In this manner, we identified a novel role of FRβ: that is, we report functional interactions of FRβ with receptors mediating cellular adhesion, in particular the CD11b/CD18 β2 integrin heterodimer complement receptor type 3/Mac-1. This interaction results in impeded adhesion of FRβ(+) human primary macrophages and THP-1 cells to collagen in comparison with their FRβ(-) counterparts. We further show that FRβ is only expressed by human macrophages when differentiated with M-CSF. These findings thus identify FRβ as a novel CD11b/CD18 regulator for trafficking and homing of a subset of macrophages on collagen.
- 650 _2
- $a antigeny CD11b $x fyziologie $7 D039481
- 650 _2
- $a antigeny CD18 $x fyziologie $7 D018821
- 650 _2
- $a buněčná adheze $7 D002448
- 650 _2
- $a pohyb buněk $7 D002465
- 650 _2
- $a proliferace buněk $7 D049109
- 650 _2
- $a kultivované buňky $7 D002478
- 650 _2
- $a kolagen $x farmakologie $7 D003094
- 650 _2
- $a folátový receptor 2 $x fyziologie $7 D058976
- 650 _2
- $a kyselina listová $x metabolismus $7 D005492
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a makrofágy $x fyziologie $7 D008264
- 650 _2
- $a tetradekanoylforbolacetát $x farmakologie $7 D013755
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Supper, Verena $u Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria;
- 700 1_
- $a Leksa, Vladimir $u Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria; Laboratory of Molecular Immunology, Institute of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovakia;
- 700 1_
- $a Mitulovic, Goran $u Department of Clinical Chemistry and Laboratory Medicine, Medical University of Vienna, 1090 Vienna, Austria;
- 700 1_
- $a Spittler, Andreas $u Department of Surgery and Core Facility Flow Cytometry, Medical University of Vienna, 1090 Vienna, Austria; and.
- 700 1_
- $a Drbal, Karel $u EXBIO Praha, 252 42 Vestec, Czech Republic.
- 700 1_
- $a Suchanek, Miloslav $u EXBIO Praha, 252 42 Vestec, Czech Republic.
- 700 1_
- $a Ohradanova-Repic, Anna $u Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria; hannes.stockinger@meduniwien.ac.at anna.repic@meduniwien.ac.at.
- 700 1_
- $a Stockinger, Hannes $u Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, 1090 Vienna, Austria; hannes.stockinger@meduniwien.ac.at anna.repic@meduniwien.ac.at.
- 773 0_
- $w MED00002741 $t Journal of immunology (Baltimore, Md. 1950) $x 1550-6606 $g Roč. 197, č. 6 (2016), s. 2229-38
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/27534550 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20171025 $b ABA008
- 991 __
- $a 20171025123110 $b ABA008
- 999 __
- $a ok $b bmc $g 1255098 $s 992532
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 197 $c 6 $d 2229-38 $e 20160817 $i 1550-6606 $m The Journal of immunology $n J Immunol $x MED00002741
- LZP __
- $a Pubmed-20171025