• Je něco špatně v tomto záznamu ?

Physiological roles of sigma factor SigD in Corynebacterium glutamicum

H. Taniguchi, T. Busche, T. Patschkowski, K. Niehaus, M. Pátek, J. Kalinowski, VF. Wendisch,

. 2017 ; 17 (1) : 158. [pub] 20170712

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18010422

BACKGROUND: Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail; however, the role of SigD in transcriptional regulation in C. glutamicum has been mostly unknown. RESULTS: In this work, pleiotropic effects of sigD overexpression at the level of phenotype, transcripts, proteins and metabolites were investigated. Overexpression of sigD decreased the growth rate of C. glutamicum cultures, and induced several physiological effects such as reduced culture foaming, turbid supernatant and cell aggregation. Upon overexpression of sigD, the level of Cmt1 (corynomycolyl transferase) in the supernatant was notably enhanced, and carbohydrate-containing compounds were excreted to the supernatant. The real-time PCR analysis revealed that sigD overexpression increased the expression of genes related to corynomycolic acid synthesis (fadD2, pks), genes encoding corynomycolyl transferases (cop1, cmt1, cmt2, cmt3), L, D-transpeptidase (lppS), a subunit of the major cell wall channel (porH), and the envelope lipid regulation factor (elrF). Furthermore, overexpression of sigD resulted in trehalose dicorynomycolate accumulation in the cell envelope. CONCLUSIONS: This study demonstrated that SigD regulates the synthesis of corynomycolate and related compounds, and expanded the knowledge of regulatory functions of sigma factors in C. glutamicum.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18010422
003      
CZ-PrNML
005      
20180418095637.0
007      
ta
008      
180404s2017 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1186/s12866-017-1067-6 $2 doi
035    __
$a (PubMed)28701150
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Taniguchi, Hironori $u Genetics of Prokaryotes, Faculty of Biology, Bielefeld University, Bielefeld, Germany. Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
245    10
$a Physiological roles of sigma factor SigD in Corynebacterium glutamicum / $c H. Taniguchi, T. Busche, T. Patschkowski, K. Niehaus, M. Pátek, J. Kalinowski, VF. Wendisch,
520    9_
$a BACKGROUND: Sigma factors are one of the components of RNA polymerase holoenzymes, and an essential factor of transcription initiation in bacteria. Corynebacterium glutamicum possesses seven genes coding for sigma factors, most of which have been studied to some detail; however, the role of SigD in transcriptional regulation in C. glutamicum has been mostly unknown. RESULTS: In this work, pleiotropic effects of sigD overexpression at the level of phenotype, transcripts, proteins and metabolites were investigated. Overexpression of sigD decreased the growth rate of C. glutamicum cultures, and induced several physiological effects such as reduced culture foaming, turbid supernatant and cell aggregation. Upon overexpression of sigD, the level of Cmt1 (corynomycolyl transferase) in the supernatant was notably enhanced, and carbohydrate-containing compounds were excreted to the supernatant. The real-time PCR analysis revealed that sigD overexpression increased the expression of genes related to corynomycolic acid synthesis (fadD2, pks), genes encoding corynomycolyl transferases (cop1, cmt1, cmt2, cmt3), L, D-transpeptidase (lppS), a subunit of the major cell wall channel (porH), and the envelope lipid regulation factor (elrF). Furthermore, overexpression of sigD resulted in trehalose dicorynomycolate accumulation in the cell envelope. CONCLUSIONS: This study demonstrated that SigD regulates the synthesis of corynomycolate and related compounds, and expanded the knowledge of regulatory functions of sigma factors in C. glutamicum.
650    _2
$a bakteriální proteiny $x genetika $x metabolismus $7 D001426
650    _2
$a Corynebacterium glutamicum $x genetika $x růst a vývoj $x metabolismus $7 D048230
650    _2
$a regulace genové exprese u bakterií $7 D015964
650    _2
$a kyseliny mykolové $x metabolismus $7 D009171
650    _2
$a sigma faktor $x genetika $x metabolismus $7 D012808
655    _2
$a časopisecké články $7 D016428
700    1_
$a Busche, Tobias $u Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
700    1_
$a Patschkowski, Thomas $u Center for Biotechnology, Bielefeld University, Bielefeld, Germany. Proteome and Metabolome Research, Faculty of Biology, Bielefeld University, Bielefeld, Germany.
700    1_
$a Niehaus, Karsten $u Center for Biotechnology, Bielefeld University, Bielefeld, Germany. Proteome and Metabolome Research, Faculty of Biology, Bielefeld University, Bielefeld, Germany.
700    1_
$a Pátek, Miroslav $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
700    1_
$a Kalinowski, Jörn $u Center for Biotechnology, Bielefeld University, Bielefeld, Germany.
700    1_
$a Wendisch, Volker F $u Genetics of Prokaryotes, Faculty of Biology, Bielefeld University, Bielefeld, Germany. volker.wendisch@uni-bielefeld.de. Center for Biotechnology, Bielefeld University, Bielefeld, Germany. volker.wendisch@uni-bielefeld.de.
773    0_
$w MED00008191 $t BMC microbiology $x 1471-2180 $g Roč. 17, č. 1 (2017), s. 158
856    41
$u https://pubmed.ncbi.nlm.nih.gov/28701150 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20180404 $b ABA008
991    __
$a 20180418095737 $b ABA008
999    __
$a ok $b bmc $g 1287907 $s 1007234
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 17 $c 1 $d 158 $e 20170712 $i 1471-2180 $m BMC microbiology $n BMC Microbiol $x MED00008191
LZP    __
$a Pubmed-20180404

Najít záznam

Citační ukazatele

Možnosti archivace