• Je něco špatně v tomto záznamu ?

Mosquitoes in the Danube Delta: searching for vectors of filarioid helminths and avian malaria

AM. Ionică, C. Zittra, V. Wimmer, N. Leitner, J. Votýpka, D. Modrý, AD. Mihalca, HP. Fuehrer,

. 2017 ; 10 (1) : 324. [pub] 20170705

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18010429

BACKGROUND: Mosquitoes are arthropods of major importance to animal and human health because they are able to transmit pathogenic agents such as filarioids (Spirurida), vector-borne nematodes, which reside in the tissues of vertebrates. In Europe, recent research has mostly focused on mosquito-borne zoonotic species, while others remain neglected. Mosquitoes are also vectors of avian malaria, which has an almost worldwide distribution, and is caused by several Plasmodium species and lineages, the most common being P. relictum. The Danube Delta region of Romania is one of the most important stopover sites for migratory birds. The local mosquito fauna is diverse and well represented, while filarial infections are known to be endemic in domestic dogs in this area. The aim of the present study was thus to assess the potential vector capacity for various filarial helminths and avian malaria of mosquitoes trapped in the Danube Delta. METHODS: In July 2015, mosquitoes were collected at seven sites located in and around a rural locality in the Danube Delta region of Romania, using CO2-baited traps and hand aspirators. Additionally, a trap was placed next to a microfilaremic dog co-infected with Dirofilaria repens and D. immitis. All randomly trapped mosquitoes were identified to the species level and pooled according to date, sampling site, and taxon. Three hundred individual mosquitoes sampled next to the microfilaremic dog were processed individually and divided into abdomen and thorax/head. Following DNA extraction, all samples were screened for the presence of DNA of filarioid helminths and avian malaria agents by PCR techniques. RESULTS: All 284 pools (a total of 5855 mosquitoes) were negative for filarioid DNA. One pool of Culex modestus mosquitoes was positive for Plasmodium sp. lineage Donana03. In the individually extracted mosquitoes, one abdomen of Aedes vexans was positive for D. repens DNA, one thorax/head of Ae. vexans was positive for DNA of Setaria labiatopapillosa, and two thorax/head of Cx. pipiens f. pipiens were positive for P. relictum lineage pSGS1. CONCLUSION: The present study suggests the vector competence of Cx. modestus and Cx. pipiens for avian Plasmodium including pathogenic species P. relictum and Ae. vexans for mammalian filarioids. Moreover, it indicates the role of Cx. pipiens f. pipiens as a potential natural vector of P. relictum lineage pSGS1 in nature.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18010429
003      
CZ-PrNML
005      
20180404142029.0
007      
ta
008      
180404s2017 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1186/s13071-017-2264-8 $2 doi
035    __
$a (PubMed)28679443
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Ionică, Angela Monica $u Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 335700, Cluj-Napoca, Romania.
245    10
$a Mosquitoes in the Danube Delta: searching for vectors of filarioid helminths and avian malaria / $c AM. Ionică, C. Zittra, V. Wimmer, N. Leitner, J. Votýpka, D. Modrý, AD. Mihalca, HP. Fuehrer,
520    9_
$a BACKGROUND: Mosquitoes are arthropods of major importance to animal and human health because they are able to transmit pathogenic agents such as filarioids (Spirurida), vector-borne nematodes, which reside in the tissues of vertebrates. In Europe, recent research has mostly focused on mosquito-borne zoonotic species, while others remain neglected. Mosquitoes are also vectors of avian malaria, which has an almost worldwide distribution, and is caused by several Plasmodium species and lineages, the most common being P. relictum. The Danube Delta region of Romania is one of the most important stopover sites for migratory birds. The local mosquito fauna is diverse and well represented, while filarial infections are known to be endemic in domestic dogs in this area. The aim of the present study was thus to assess the potential vector capacity for various filarial helminths and avian malaria of mosquitoes trapped in the Danube Delta. METHODS: In July 2015, mosquitoes were collected at seven sites located in and around a rural locality in the Danube Delta region of Romania, using CO2-baited traps and hand aspirators. Additionally, a trap was placed next to a microfilaremic dog co-infected with Dirofilaria repens and D. immitis. All randomly trapped mosquitoes were identified to the species level and pooled according to date, sampling site, and taxon. Three hundred individual mosquitoes sampled next to the microfilaremic dog were processed individually and divided into abdomen and thorax/head. Following DNA extraction, all samples were screened for the presence of DNA of filarioid helminths and avian malaria agents by PCR techniques. RESULTS: All 284 pools (a total of 5855 mosquitoes) were negative for filarioid DNA. One pool of Culex modestus mosquitoes was positive for Plasmodium sp. lineage Donana03. In the individually extracted mosquitoes, one abdomen of Aedes vexans was positive for D. repens DNA, one thorax/head of Ae. vexans was positive for DNA of Setaria labiatopapillosa, and two thorax/head of Cx. pipiens f. pipiens were positive for P. relictum lineage pSGS1. CONCLUSION: The present study suggests the vector competence of Cx. modestus and Cx. pipiens for avian Plasmodium including pathogenic species P. relictum and Ae. vexans for mammalian filarioids. Moreover, it indicates the role of Cx. pipiens f. pipiens as a potential natural vector of P. relictum lineage pSGS1 in nature.
650    _2
$a Aedes $x klasifikace $x parazitologie $7 D000330
650    _2
$a zvířata $7 D000818
650    _2
$a Culex $x klasifikace $x parazitologie $7 D003465
650    _2
$a Culicidae $x klasifikace $x parazitologie $7 D009033
650    _2
$a Dirofilaria immitis $x genetika $x izolace a purifikace $7 D004183
650    _2
$a Dirofilaria repens $x genetika $x izolace a purifikace $7 D060348
650    _2
$a psi $7 D004285
650    _2
$a filarióza $x epidemiologie $x parazitologie $7 D005368
650    _2
$a Filarioidea $x genetika $x izolace a purifikace $7 D005370
650    _2
$a lidé $7 D006801
650    _2
$a hmyz - vektory $x parazitologie $7 D007303
650    _2
$a malárie ptačí $x epidemiologie $x parazitologie $7 D008289
650    _2
$a Plasmodium $x genetika $x izolace a purifikace $7 D010961
650    _2
$a Rumunsko $x epidemiologie $7 D012383
650    _2
$a Setaria (Nematoda) $x genetika $x izolace a purifikace $7 D017182
655    _2
$a časopisecké články $7 D016428
700    1_
$a Zittra, Carina $u Department of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, Vienna, Austria.
700    1_
$a Wimmer, Victoria $u Department of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, Vienna, Austria.
700    1_
$a Leitner, Natascha $u Department of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, Vienna, Austria.
700    1_
$a Votýpka, Jan $u Department of Parasitology, Faculty of Science, Charles University, Prague, Czech Republic. Institute of Parasitology, Biology Centre of Czech Academy of Sciences, České Budĕjovice, Czech Republic.
700    1_
$a Modrý, David $u Department of Pathology and Parasitology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. CEITEC-VFU, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. Institute of Parasitology, Biology Centre of Czech Academy of Sciences, České Budĕjovice, Czech Republic.
700    1_
$a Mihalca, Andrei Daniel $u Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 335700, Cluj-Napoca, Romania.
700    1_
$a Fuehrer, Hans-Peter $u Department of Pathobiology, Institute of Parasitology, University of Veterinary Medicine Vienna, Vienna, Austria. Hans-Peter.Fuehrer@vetmeduni.ac.at.
773    0_
$w MED00165371 $t Parasites & vectors $x 1756-3305 $g Roč. 10, č. 1 (2017), s. 324
856    41
$u https://pubmed.ncbi.nlm.nih.gov/28679443 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20180404 $b ABA008
991    __
$a 20180404142109 $b ABA008
999    __
$a ok $b bmc $g 1287914 $s 1007241
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 10 $c 1 $d 324 $e 20170705 $i 1756-3305 $m Parasites & vectors $n Parasit Vectors $x MED00165371
LZP    __
$a Pubmed-20180404

Najít záznam

Citační ukazatele

Možnosti archivace