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Interaction of human osteoblast-like Saos-2 cells with stainless steel coated by silicalite-1 films
I. Jirka, M. Vandrovcová, J. Plšek, M. Bouša, L. Brabec, H. Dragounová, L. Bačáková,
Language English Country Netherlands
Document type Journal Article
- MeSH
- Cell Adhesion MeSH
- Cell Line MeSH
- Photoelectron Spectroscopy MeSH
- Humans MeSH
- Microscopy, Electron, Scanning MeSH
- Stainless Steel MeSH
- Osteoblasts * MeSH
- Silicon Dioxide MeSH
- Surface Properties MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
This paper investigates the interaction of human osteoblast-like Saos-2 cells with stainless steel covered by a film of densely inter-grown silicalite-1 crystals with defined outer and inner surfaces. The chemical composition of this film, labeled as SF(RT), was tuned by heat treatment at 300°C and 500°C (labeled as SF(300) and SF(500), respectively) and characterized by X-ray photoelectron spectroscopy (XPS), water drop contact angle (WCA) measurements and scanning electron microscopy (SEM). The number, the spreading area and the activity of alkaline phosphatase of human osteoblast-like Saos-2 cells in cultures on the silicalite-1 film were affected by the chemical composition of its outer surface and by its micro-porous structure. The number and the spreading area of the adhered osteoblast-like cells on day 1 was highest on the surface of SF(RT) relative to their adhesion and spreading on a glass cover slip due to the SF(RT) topology. However, SF(300) markedly supported cell growth during days 3 and 7 after seeding.
Institute of Physiology ASCR v v i Vídeňská 1083 142 20 Prague 4 Czech Republic
J Heyrovský Institute of Physical Chemistry ASCR v v i Dolejškova 3 182 23 Prague 8 Czech Republic
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