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Electrochemical and optical study of metallothionein interactions with prion proteins

A. Cardova, P. Adam, S. Mariani, L. Richtera, Z. Heger, J. Labuda, M. Minunni, V. Adam,

. 2017 ; 140 (-) : 355-361. [pub] 20170323

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18010686

Grantová podpora
NV15-28334A MZ0 CEP - Centrální evidence projektů

The prion protein (PrPC) can be structurally shifted to its PrPScisoform causing a wide range of neurodegenerative diseases, which are currently incurable. There is an evidence that metallothioneins (MTs), and especially MT-3, are associated with neurodegenerative diseases. PrPCand MTs play pivotal roles in maintaining metal homeostasis; therefore, it is conceivable that each of them has its own significance in prion diseases. In this paper, we study the nature of interactions between PrPC, MT, and copper ions, Cu(II), using the method of differential pulse voltammetry (DPV) coupled with adsorptive transfer stripping technique (AdTS). Electrochemical properties of PrP itself and its interactions with both the Cu(II) ions and MTs have been found. Based on the results obtained, we hypothesised the formation of the complex in molar ratio 2:1 (PrPC:MT). Surface plasmon resonance imaging (SPRi) was used as a control reference assay to further confirm results obtained by the electrochemical approach, such as the specific interactions between PrPCand MT-3.

Citace poskytuje Crossref.org

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$a The prion protein (PrPC) can be structurally shifted to its PrPScisoform causing a wide range of neurodegenerative diseases, which are currently incurable. There is an evidence that metallothioneins (MTs), and especially MT-3, are associated with neurodegenerative diseases. PrPCand MTs play pivotal roles in maintaining metal homeostasis; therefore, it is conceivable that each of them has its own significance in prion diseases. In this paper, we study the nature of interactions between PrPC, MT, and copper ions, Cu(II), using the method of differential pulse voltammetry (DPV) coupled with adsorptive transfer stripping technique (AdTS). Electrochemical properties of PrP itself and its interactions with both the Cu(II) ions and MTs have been found. Based on the results obtained, we hypothesised the formation of the complex in molar ratio 2:1 (PrPC:MT). Surface plasmon resonance imaging (SPRi) was used as a control reference assay to further confirm results obtained by the electrochemical approach, such as the specific interactions between PrPCand MT-3.
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$a Adam, Pavlina $u Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
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$a Mariani, Stefano $u Dipartimento di Chimica "Ugo Schiff" and CSGI, Università di Firenze, Sesto Fiorentino, Italy.
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$a Richtera, Lukas $u Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
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$a Heger, Zbynek $u Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic.
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$a Labuda, Jan $u Institute of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Bratislava, Slovakia.
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$a Minunni, Maria $u Dipartimento di Chimica "Ugo Schiff" and CSGI, Università di Firenze, Sesto Fiorentino, Italy.
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$a Adam, Vojtech $u Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic. Electronic address: vojtech.adam@mendelu.cz.
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