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A fully validated bioanalytical method using an UHPLC-MS/MS system for quantification of DNA and RNA oxidative stress biomarkers
B. Cervinkova, LK. Krcmova, V. Sestakova, D. Solichova, P. Solich,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, validační studie
NLK
ProQuest Central
od 2013-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2003-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2013-01-01 do Před 1 rokem
- MeSH
- biologické markery moč MeSH
- deoxyguanosin analogy a deriváty moč MeSH
- DNA moč MeSH
- dospělí MeSH
- extrakce na pevné fázi metody MeSH
- guanosin analogy a deriváty moč MeSH
- kreatin moč MeSH
- lidé MeSH
- limita detekce MeSH
- mladý dospělý MeSH
- nádory moč MeSH
- neurodegenerativní nemoci moč MeSH
- oxidační stres MeSH
- RNA moč MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- validační studie MeSH
A new, rapid and effective ultra-high-performance liquid chromatography method with mass spectrometry detection is described for the separation and quantification of 8-hydroxy-2-deoxyguanosine, 8-hydroxyguanosine and creatinine in human urine. The present study uses an isotope-labelled internal standard ([15N]5-8-hydroxy-2-deoxyguanosine), a BIO core-shell stationary phase and an isocratic elution of methanol and water. Sample preparation of human urine was performed by solid-phase extraction (SPE) on Oasis HLB cartridges with methanol/water 50:50 (v/v) elution. Extraction recoveries ranged from 98.1% to 109.2%. Biological extracts showed high short-term stability. Several aspects of this procedure make it suitable for both clinical and research purposes: a short elution time of less than 3.2 min, an intra-day precision of 2.5-8.9%, an inter-day precision of 3.4-8.7% and low limits of quantification (27.7 nM for 8-hydroxyguanosine, 6.0 nM for 8-hydroxy-2-deoxyguanosine). Finally, simultaneous analysis of DNA and RNA oxidative stress biomarkers is a useful tool for monitoring disease progression in neurodegenerative disorders and cancer. Graphical abstract UHPLC-MS/MS analysis of DNA and RNA oxidative stress biomarkers.
Citace poskytuje Crossref.org
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- $a Cervinkova, Barbora $u Department of Analytical Chemistry, Faculty of Pharmacy, Charles University, Akademika Heyrovskeho 1203, 50005, Hradec Kralove, Czech Republic. 3rd Internal Gerontometabolic Clinic, University Hospital, Sokolska 581, 50005, Hradec Kralove, Czech Republic.
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- $a A fully validated bioanalytical method using an UHPLC-MS/MS system for quantification of DNA and RNA oxidative stress biomarkers / $c B. Cervinkova, LK. Krcmova, V. Sestakova, D. Solichova, P. Solich,
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- $a A new, rapid and effective ultra-high-performance liquid chromatography method with mass spectrometry detection is described for the separation and quantification of 8-hydroxy-2-deoxyguanosine, 8-hydroxyguanosine and creatinine in human urine. The present study uses an isotope-labelled internal standard ([15N]5-8-hydroxy-2-deoxyguanosine), a BIO core-shell stationary phase and an isocratic elution of methanol and water. Sample preparation of human urine was performed by solid-phase extraction (SPE) on Oasis HLB cartridges with methanol/water 50:50 (v/v) elution. Extraction recoveries ranged from 98.1% to 109.2%. Biological extracts showed high short-term stability. Several aspects of this procedure make it suitable for both clinical and research purposes: a short elution time of less than 3.2 min, an intra-day precision of 2.5-8.9%, an inter-day precision of 3.4-8.7% and low limits of quantification (27.7 nM for 8-hydroxyguanosine, 6.0 nM for 8-hydroxy-2-deoxyguanosine). Finally, simultaneous analysis of DNA and RNA oxidative stress biomarkers is a useful tool for monitoring disease progression in neurodegenerative disorders and cancer. Graphical abstract UHPLC-MS/MS analysis of DNA and RNA oxidative stress biomarkers.
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