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EVI2B is a C/EBPα target gene required for granulocytic differentiation and functionality of hematopoietic progenitors
P. Zjablovskaja, M. Kardosova, P. Danek, P. Angelisova, T. Benoukraf, AA. Wurm, T. Kalina, S. Sian, M. Balastik, R. Delwel, T. Brdicka, DG. Tenen, G. Behre, F. Fiore, B. Malissen, V. Horejsi, M. Alberich-Jorda,
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
NV15-26588A
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Zdroj
NLK
Free Medical Journals
od 2011
PubMed Central
od 2011 do Před 1 rokem
Europe PubMed Central
od 2011 do Před 1 rokem
ProQuest Central
od 2000-01-01 do Před 1 rokem
Open Access Digital Library
od 1997-01-01
Health & Medicine (ProQuest)
od 2000-01-01 do Před 1 rokem
PubMed
28186500
DOI
10.1038/cdd.2017.6
Knihovny.cz E-zdroje
- MeSH
- akutní myeloidní leukemie metabolismus patologie MeSH
- apoptóza MeSH
- buněčná diferenciace účinky léků MeSH
- buňky kostní dřeně cytologie MeSH
- down regulace účinky léků MeSH
- estradiol farmakologie MeSH
- faktor stimulující kolonie granulocytů farmakologie MeSH
- granulocyty cytologie metabolismus MeSH
- hematopoetické kmenové buňky cytologie metabolismus MeSH
- kultivované buňky MeSH
- lidé MeSH
- malá interferující RNA metabolismus MeSH
- membránové glykoproteiny antagonisté a inhibitory genetika metabolismus MeSH
- membránové proteiny antagonisté a inhibitory genetika metabolismus MeSH
- myši inbrední C57BL MeSH
- myši knockoutované MeSH
- myši MeSH
- proliferace buněk účinky léků MeSH
- promotorové oblasti (genetika) MeSH
- protein alfa vázající zesilovač transkripce CCAAT genetika metabolismus MeSH
- RNA interference MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Development of hematopoietic populations through the process of differentiation is critical for proper hematopoiesis. The transcription factor CCAAT/enhancer binding protein alpha (C/EBPα) is a master regulator of myeloid differentiation, and the identification of C/EBPα target genes is key to understand this process. Here we identified the Ecotropic Viral Integration Site 2B (EVI2B) gene as a direct target of C/EBPα. We showed that the product of the gene, the transmembrane glycoprotein EVI2B (CD361), is abundantly expressed on the surface of primary hematopoietic cells, the highest levels of expression being reached in mature granulocytes. Using shRNA-mediated downregulation of EVI2B in human and murine cell lines and in primary hematopoietic stem and progenitor cells, we demonstrated impaired myeloid lineage development and altered progenitor functions in EVI2B-silenced cells. We showed that the compromised progenitor functionality in Evi2b-depleted cells can be in part explained by deregulation of cell proliferation and apoptosis. In addition, we generated an Evi2b knockout murine model and demonstrated altered properties of hematopoietic progenitors, as well as impaired G-CSF dependent myeloid colony formation in the knockout cells. Remarkably, we found that EVI2B is significantly downregulated in human acute myeloid leukemia samples characterized by defects in CEBPA. Altogether, our data demonstrate that EVI2B is a downstream target of C/EBPα, which regulates myeloid differentiation and functionality of hematopoietic progenitors.
Centre d'Immunophénomique Aix Marseille Université Inserm CNRS Marseille F 13288 France
Division of Hematology and Oncology Leipzig University Hospital Leipzig Germany
Citace poskytuje Crossref.org
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