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Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples
M. Bocková, X. Chadtová Song, E. Gedeonová, K. Levová, M. Kalousová, T. Zima, J. Homola,
Jazyk angličtina Země Německo
Typ dokumentu hodnotící studie, časopisecké články
NLK
ProQuest Central
od 2011-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2003-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 2011-01-01 do Před 1 rokem
- MeSH
- kovové nanočástice chemie MeSH
- lidé MeSH
- limita detekce MeSH
- povrchová plasmonová rezonance metody MeSH
- pufry MeSH
- těhotenský plazmatický protein A analýza MeSH
- těhotenství MeSH
- zlato chemie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men. Graphical Abstract Temporal sensor response corresponding to respective steps of the assay for detection of PAPP-A2 in buffer.
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- $a Bocková, Markéta $u Department of Optical Sensors, Institute of Photonics and Electronics of the CAS, Chaberska 57, 18251, Prague 8, Czech Republic.
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- $a Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples / $c M. Bocková, X. Chadtová Song, E. Gedeonová, K. Levová, M. Kalousová, T. Zima, J. Homola,
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- $a Pregnancy associated plasma protein A2 (PAPP-A2) is a metalloproteinase that plays multiple roles in fetal development and post-natal growth. Here we present a novel surface plasmon resonance (SPR) biosensor for the rapid and quantitative detection of PAPP-A2 in blood samples. This biosensor uses a single surface referencing approach and a sandwich assay with functionalized gold nanoparticles for signal enhancement. We demonstrate that this SPR biosensor enables the detection of PAPP-A2 in 30 % blood plasma at levels as low as 3.6 ng/mL. We also characterize the performance of the biosensor and evaluate its cross-reactivity to a PAPP-A analogue. Finally, we utilize this SPR biosensor for the detection of PAPP-A2 in blood serum from two groups of subjects: pregnant women and healthy non-pregnant women and men. Graphical Abstract Temporal sensor response corresponding to respective steps of the assay for detection of PAPP-A2 in buffer.
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- $a Chadtová Song, Xue $u Department of Optical Sensors, Institute of Photonics and Electronics of the CAS, Chaberska 57, 18251, Prague 8, Czech Republic.
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- $a Hemmerová, Erika, $u Department of Optical Sensors, Institute of Photonics and Electronics of the CAS, Chaberska 57, 18251, Prague 8, Czech Republic. $d 1991- $7 xx0269692
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- $a Levová, Kateřina $u Institute of Medical Biochemistry and Laboratory Diagnostics of First Faculty of Medicine, Charles University in Prague and the General University Hospital in Prague, U Nemocnice 2, 12808, Praha 2, Czech Republic.
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- $a Kalousová, Marta $u Institute of Medical Biochemistry and Laboratory Diagnostics of First Faculty of Medicine, Charles University in Prague and the General University Hospital in Prague, U Nemocnice 2, 12808, Praha 2, Czech Republic.
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- $a Zima, Tomáš $u Institute of Medical Biochemistry and Laboratory Diagnostics of First Faculty of Medicine, Charles University in Prague and the General University Hospital in Prague, U Nemocnice 2, 12808, Praha 2, Czech Republic.
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- $a Homola, Jiří $u Department of Optical Sensors, Institute of Photonics and Electronics of the CAS, Chaberska 57, 18251, Prague 8, Czech Republic. homola@ufe.cz.
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