-
Je něco špatně v tomto záznamu ?
Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC-MS-(TOF)
J. Kasparovska, L. Krizova, J. Lochman, K. Dadakova, T. Kasparovsky,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
PubMed
27021208
DOI
10.1093/chromsci/bmw033
Knihovny.cz E-zdroje
- MeSH
- bachor MeSH
- chemické techniky analytické metody MeSH
- chromatografie kapalinová * MeSH
- gastrointestinální obsah chemie MeSH
- Glycine max chemie metabolismus MeSH
- isoflavony analýza MeSH
- kinetika MeSH
- mléko chemie MeSH
- skot MeSH
- tandemová hmotnostní spektrometrie * MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Soybean-derived isoflavones belong to the family of biologically active phytoestrogens. The purpose of this study was to develop a sensitive method, which permits quantification of the soybean isoflavonoids and equol in bovine rumen fluid and milk using LC-MS-(TOF). The samples of rumen fluid and milk were obtained from 12 lactating dairy cows ingesting 7,500-9,500 mg of total isoflavones daily. The validation of the developed method showed the limits of quantification to be in the range of 0.9-5.0 ng/mL. The precision was determined as relative standard deviation, which was lower than 25% in all cases. The recoveries of the most isoflavonoids were satisfactory. Lower recoveries of daidzin and glycitin can be solved by adding an internal standard. The presented method will be useful for kinetic studies of isoflavone metabolism in ruminants due to simultaneous quantification of free aglycones and glycosides in the rumen fluid.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18011274
- 003
- CZ-PrNML
- 005
- 20180426104005.0
- 007
- ta
- 008
- 180404s2016 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1093/chromsci/bmw033 $2 doi
- 035 __
- $a (PubMed)27021208
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Kasparovska, Jitka $u Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic.
- 245 10
- $a Soybean-Derived Isoflavone Determination in Rumen Fluid and Milk by LC-MS-(TOF) / $c J. Kasparovska, L. Krizova, J. Lochman, K. Dadakova, T. Kasparovsky,
- 520 9_
- $a Soybean-derived isoflavones belong to the family of biologically active phytoestrogens. The purpose of this study was to develop a sensitive method, which permits quantification of the soybean isoflavonoids and equol in bovine rumen fluid and milk using LC-MS-(TOF). The samples of rumen fluid and milk were obtained from 12 lactating dairy cows ingesting 7,500-9,500 mg of total isoflavones daily. The validation of the developed method showed the limits of quantification to be in the range of 0.9-5.0 ng/mL. The precision was determined as relative standard deviation, which was lower than 25% in all cases. The recoveries of the most isoflavonoids were satisfactory. Lower recoveries of daidzin and glycitin can be solved by adding an internal standard. The presented method will be useful for kinetic studies of isoflavone metabolism in ruminants due to simultaneous quantification of free aglycones and glycosides in the rumen fluid.
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a skot $7 D002417
- 650 _2
- $a chemické techniky analytické $x metody $7 D002623
- 650 12
- $a chromatografie kapalinová $7 D002853
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a gastrointestinální obsah $x chemie $7 D005766
- 650 _2
- $a isoflavony $x analýza $7 D007529
- 650 _2
- $a kinetika $7 D007700
- 650 _2
- $a mléko $x chemie $7 D008892
- 650 _2
- $a bachor $7 D012417
- 650 _2
- $a Glycine max $x chemie $x metabolismus $7 D013025
- 650 12
- $a tandemová hmotnostní spektrometrie $7 D053719
- 655 _2
- $a časopisecké články $7 D016428
- 700 1_
- $a Krizova, Ludmila $u Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic.
- 700 1_
- $a Lochman, Jan $u Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic.
- 700 1_
- $a Dadakova, Katerina $u Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic.
- 700 1_
- $a Kasparovsky, Tomas $u Department of Biochemistry, Faculty of Science, Masaryk University, Kotlarska 2, 611 37 Brno, Czech Republic tkasp@sci.muni.cz.
- 773 0_
- $w MED00002734 $t Journal of chromatographic science $x 1945-239X $g Roč. 54, č. 6 (2016), s. 997-1003
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/27021208 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180404 $b ABA008
- 991 __
- $a 20180426104116 $b ABA008
- 999 __
- $a ok $b bmc $g 1288759 $s 1008086
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 54 $c 6 $d 997-1003 $e 20160328 $i 1945-239X $m Journal of chromatographic science $n J Chromatogr Sci $x MED00002734
- LZP __
- $a Pubmed-20180404
