• Je něco špatně v tomto záznamu ?

Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes

J. Puterova, O. Razumova, T. Martinek, O. Alexandrov, M. Divashuk, Z. Kubat, R. Hobza, G. Karlov, E. Kejnovsky,

. 2017 ; 9 (1) : 197-212.

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc18016824

Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome. We carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and 45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration. Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18016824
003      
CZ-PrNML
005      
20180521101450.0
007      
ta
008      
180515s2017 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1093/gbe/evw303 $2 doi
035    __
$a (PubMed)28057732
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Puterova, Janka $u Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic. Department of Information Systems, Faculty of Information Technology, Brno University of Technology, Brno, Czech Republic.
245    10
$a Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes / $c J. Puterova, O. Razumova, T. Martinek, O. Alexandrov, M. Divashuk, Z. Kubat, R. Hobza, G. Karlov, E. Kejnovsky,
520    9_
$a Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome. We carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and 45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration. Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes.
650    12
$a chromozomy rostlin $7 D032461
650    12
$a transpozibilní elementy DNA $7 D004251
650    _2
$a DNA rostlinná $x genetika $7 D018744
650    12
$a satelitní DNA $7 D004276
650    _2
$a molekulární evoluce $7 D019143
650    _2
$a genom rostlinný $7 D018745
650    _2
$a Hippophae $x genetika $7 D031281
650    _2
$a fylogeneze $7 D010802
650    _2
$a sekvenční analýza DNA $x metody $7 D017422
650    12
$a pohlavní chromozomy $7 D012730
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Razumova, Olga $u Centre for Molecular Biotechnology, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Moscow, Russia.
700    1_
$a Martinek, Tomas $u Department of Information Systems, Faculty of Information Technology, Brno University of Technology, Brno, Czech Republic.
700    1_
$a Alexandrov, Oleg $u Centre for Molecular Biotechnology, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Moscow, Russia.
700    1_
$a Divashuk, Mikhail $u Centre for Molecular Biotechnology, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Moscow, Russia.
700    1_
$a Kubat, Zdenek $u Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
700    1_
$a Hobza, Roman $u Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic. Institute of Experimental Botany, Center of the Region Haná for Biotechnological and Agricultural Research, Olomouc, Czech Republic.
700    1_
$a Karlov, Gennady $u Centre for Molecular Biotechnology, Russian State Agrarian University - Moscow Timiryazev Agricultural Academy, Moscow, Russia. All-Russia Research Institute of Agricultural Biotechnology, Moscow, Russia.
700    1_
$a Kejnovsky, Eduard $u Department of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
773    0_
$w MED00170504 $t Genome biology and evolution $x 1759-6653 $g Roč. 9, č. 1 (2017), s. 197-212
856    41
$u https://pubmed.ncbi.nlm.nih.gov/28057732 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20180515 $b ABA008
991    __
$a 20180521101631 $b ABA008
999    __
$a ok $b bmc $g 1300448 $s 1013664
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 9 $c 1 $d 197-212 $i 1759-6653 $m Genome biology and evolution $n Genome Biol Evol $x MED00170504
LZP    __
$a Pubmed-20180515

Najít záznam

Citační ukazatele

Možnosti archivace