-
Je něco špatně v tomto záznamu ?
Laminin-511 and laminin-521-based matrices for efficient hepatic specification of human pluripotent stem cells
LK. Kanninen, R. Harjumäki, P. Peltoniemi, MS. Bogacheva, T. Salmi, P. Porola, J. Niklander, T. Smutný, A. Urtti, ML. Yliperttula, YR. Lou,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- biomimetické materiály chemie MeSH
- buněčná diferenciace fyziologie MeSH
- buněčné linie MeSH
- extracelulární matrix - proteiny metabolismus MeSH
- extracelulární matrix metabolismus MeSH
- hepatocyty cytologie fyziologie MeSH
- laminin metabolismus MeSH
- lidé MeSH
- pluripotentní kmenové buňky cytologie metabolismus MeSH
- techniky vsádkové kultivace metody MeSH
- tkáňové inženýrství metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Human pluripotent stem cells (hPSCs) have gained a solid foothold in basic research and drug industry as they can be used in vitro to study human development and have potential to offer limitless supply of various somatic cell types needed in drug development. Although the hepatic differentiation of hPSCs has been extensively studied, only a little attention has been paid to the role of the extracellular matrix. In this study we used laminin-511, laminin-521, and fibronectin, found in human liver progenitor cells, as culture matrices for hPSC-derived definitive endoderm cells. We observed that laminin-511 and laminin-521 either alone or in combination support the hepatic specification and that fibronectin is not a vital matrix protein for the hPSC-derived definitive endoderm cells. The expression of the laminin-511/521-specific integrins increased during the definitive endoderm induction and hepatic specification. The hepatic cells differentiated on laminin matrices showed the upregulation of liver-specific markers both at mRNA and protein levels, secreted human albumin, stored glycogen, and exhibited cytochrome P450 enzyme activity and inducibility. Altogether, we found that laminin-511 and laminin-521 can be used as stage-specific matrices to guide the hepatic specification of hPSC-derived definitive endoderm cells.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc18017177
- 003
- CZ-PrNML
- 005
- 20180523093339.0
- 007
- ta
- 008
- 180515s2016 ne f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1016/j.biomaterials.2016.06.054 $2 doi
- 035 __
- $a (PubMed)27372423
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a ne
- 100 1_
- $a Kanninen, Liisa K $u Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P. O. Box 56, FI-00014 Helsinki, Finland.
- 245 10
- $a Laminin-511 and laminin-521-based matrices for efficient hepatic specification of human pluripotent stem cells / $c LK. Kanninen, R. Harjumäki, P. Peltoniemi, MS. Bogacheva, T. Salmi, P. Porola, J. Niklander, T. Smutný, A. Urtti, ML. Yliperttula, YR. Lou,
- 520 9_
- $a Human pluripotent stem cells (hPSCs) have gained a solid foothold in basic research and drug industry as they can be used in vitro to study human development and have potential to offer limitless supply of various somatic cell types needed in drug development. Although the hepatic differentiation of hPSCs has been extensively studied, only a little attention has been paid to the role of the extracellular matrix. In this study we used laminin-511, laminin-521, and fibronectin, found in human liver progenitor cells, as culture matrices for hPSC-derived definitive endoderm cells. We observed that laminin-511 and laminin-521 either alone or in combination support the hepatic specification and that fibronectin is not a vital matrix protein for the hPSC-derived definitive endoderm cells. The expression of the laminin-511/521-specific integrins increased during the definitive endoderm induction and hepatic specification. The hepatic cells differentiated on laminin matrices showed the upregulation of liver-specific markers both at mRNA and protein levels, secreted human albumin, stored glycogen, and exhibited cytochrome P450 enzyme activity and inducibility. Altogether, we found that laminin-511 and laminin-521 can be used as stage-specific matrices to guide the hepatic specification of hPSC-derived definitive endoderm cells.
- 650 _2
- $a techniky vsádkové kultivace $x metody $7 D061249
- 650 _2
- $a biomimetické materiály $x chemie $7 D040761
- 650 _2
- $a buněčná diferenciace $x fyziologie $7 D002454
- 650 _2
- $a buněčné linie $7 D002460
- 650 _2
- $a extracelulární matrix $x metabolismus $7 D005109
- 650 _2
- $a extracelulární matrix - proteiny $x metabolismus $7 D016326
- 650 _2
- $a hepatocyty $x cytologie $x fyziologie $7 D022781
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a laminin $x metabolismus $7 D007797
- 650 _2
- $a pluripotentní kmenové buňky $x cytologie $x metabolismus $7 D039904
- 650 _2
- $a tkáňové inženýrství $x metody $7 D023822
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Harjumäki, Riina $u Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P. O. Box 56, FI-00014 Helsinki, Finland.
- 700 1_
- $a Peltoniemi, Pasi $u Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P. O. Box 56, FI-00014 Helsinki, Finland.
- 700 1_
- $a Bogacheva, Mariia S $u Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P. O. Box 56, FI-00014 Helsinki, Finland.
- 700 1_
- $a Salmi, Tuuli $u Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P. O. Box 56, FI-00014 Helsinki, Finland.
- 700 1_
- $a Porola, Pauliina $u Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P. O. Box 56, FI-00014 Helsinki, Finland.
- 700 1_
- $a Niklander, Johanna $u Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P. O. Box 56, FI-00014 Helsinki, Finland.
- 700 1_
- $a Smutný, Tomáš $u Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P. O. Box 56, FI-00014 Helsinki, Finland; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Charles University in Prague, 50005 Hradec Králové, Czech Republic.
- 700 1_
- $a Urtti, Arto $u Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P. O. Box 56, FI-00014 Helsinki, Finland; School of Pharmacy, University of Eastern Finland, P. O. Box 1627, 70211 Kuopio, Finland.
- 700 1_
- $a Yliperttula, Marjo L $u Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P. O. Box 56, FI-00014 Helsinki, Finland.
- 700 1_
- $a Lou, Yan-Ru $u Division of Pharmaceutical Biosciences, Centre for Drug Research, Faculty of Pharmacy, University of Helsinki, P. O. Box 56, FI-00014 Helsinki, Finland. Electronic address: yan-ru.lou@helsinki.fi.
- 773 0_
- $w MED00000753 $t Biomaterials $x 1878-5905 $g Roč. 103, č. - (2016), s. 86-100
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/27372423 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20180515 $b ABA008
- 991 __
- $a 20180523093524 $b ABA008
- 999 __
- $a ok $b bmc $g 1300801 $s 1014017
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2016 $b 103 $c - $d 86-100 $e 20160623 $i 1878-5905 $m Biomaterials $n Biomaterials $x MED00000753
- LZP __
- $a Pubmed-20180515
