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Evolutionary Conserved Cysteines Function as cis-Acting Regulators of Arabidopsis PIN-FORMED 2 Distribution

K. Retzer, J. Lacek, R. Skokan, CI. Del Genio, S. Vosolsobě, M. Laňková, K. Malínská, N. Konstantinova, E. Zažímalová, RM. Napier, J. Petrášek, C. Luschnig,

. 2017 ; 18 (11) : . [pub] 20171029

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc18024503

Coordination of plant development requires modulation of growth responses that are under control of the phytohormone auxin. PIN-FORMED plasma membrane proteins, involved in intercellular transport of the growth regulator, are key to the transmission of such auxin signals and subject to multilevel surveillance mechanisms, including reversible post-translational modifications. Apart from well-studied PIN protein modifications, namely phosphorylation and ubiquitylation, no further post-translational modifications have been described so far. Here, we focused on root-specific Arabidopsis PIN2 and explored functional implications of two evolutionary conserved cysteines, by a combination of in silico and molecular approaches. PIN2 sequence alignments and modeling predictions indicated that both cysteines are facing the cytoplasm and therefore would be accessible to redox status-controlled modifications. Notably, mutant pin2C-A alleles retained functionality, demonstrated by their ability to almost completely rescue defects of a pin2 null allele, whereas high resolution analysis of pin2C-A localization revealed increased intracellular accumulation, and altered protein distribution within plasma membrane micro-domains. The observed effects of cysteine replacements on root growth and PIN2 localization are consistent with a model in which redox status-dependent cysteine modifications participate in the regulation of PIN2 mobility, thereby fine-tuning polar auxin transport.

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$a Retzer, Katarzyna $u Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria. retzer@ueb.cas.cz. Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. retzer@ueb.cas.cz.
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$a Evolutionary Conserved Cysteines Function as cis-Acting Regulators of Arabidopsis PIN-FORMED 2 Distribution / $c K. Retzer, J. Lacek, R. Skokan, CI. Del Genio, S. Vosolsobě, M. Laňková, K. Malínská, N. Konstantinova, E. Zažímalová, RM. Napier, J. Petrášek, C. Luschnig,
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$a Coordination of plant development requires modulation of growth responses that are under control of the phytohormone auxin. PIN-FORMED plasma membrane proteins, involved in intercellular transport of the growth regulator, are key to the transmission of such auxin signals and subject to multilevel surveillance mechanisms, including reversible post-translational modifications. Apart from well-studied PIN protein modifications, namely phosphorylation and ubiquitylation, no further post-translational modifications have been described so far. Here, we focused on root-specific Arabidopsis PIN2 and explored functional implications of two evolutionary conserved cysteines, by a combination of in silico and molecular approaches. PIN2 sequence alignments and modeling predictions indicated that both cysteines are facing the cytoplasm and therefore would be accessible to redox status-controlled modifications. Notably, mutant pin2C-A alleles retained functionality, demonstrated by their ability to almost completely rescue defects of a pin2 null allele, whereas high resolution analysis of pin2C-A localization revealed increased intracellular accumulation, and altered protein distribution within plasma membrane micro-domains. The observed effects of cysteine replacements on root growth and PIN2 localization are consistent with a model in which redox status-dependent cysteine modifications participate in the regulation of PIN2 mobility, thereby fine-tuning polar auxin transport.
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$a Lacek, Jozef $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. lacek@ueb.cas.cz. Department of Experimental Plant Biology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic. lacek@ueb.cas.cz.
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$a Skokan, Roman $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. skokan@ueb.cas.cz. Department of Experimental Plant Biology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic. skokan@ueb.cas.cz.
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$a Del Genio, Charo I $u School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK. the.paraw@gmail.com.
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$a Vosolsobě, Stanislav $u Department of Experimental Plant Biology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic. stanislav.vosolsobe@natur.cuni.cz.
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$a Laňková, Martina $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. lankova@ueb.cas.cz.
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$a Malínská, Kateřina $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. malinska@ueb.cas.cz.
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$a Konstantinova, Nataliia $u Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria. nataliia.konstantinova@boku.ac.at.
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$a Zažímalová, Eva $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. Zazimalova@ueb.cas.cz.
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$a Napier, Richard M $u School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK. Richard.Napier@warwick.ac.uk.
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$a Petrášek, Jan $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. petrasek@ueb.cas.cz. Department of Experimental Plant Biology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic. petrasek@ueb.cas.cz.
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$a Luschnig, Christian $u Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria. christian.luschnig@boku.ac.at.
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