• Je něco špatně v tomto záznamu ?

Evolutionary Conserved Cysteines Function as cis-Acting Regulators of Arabidopsis PIN-FORMED 2 Distribution

K. Retzer, J. Lacek, R. Skokan, CI. Del Genio, S. Vosolsobě, M. Laňková, K. Malínská, N. Konstantinova, E. Zažímalová, RM. Napier, J. Petrášek, C. Luschnig,

. 2017 ; 18 (11) : . [pub] 20171029

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18024503

Coordination of plant development requires modulation of growth responses that are under control of the phytohormone auxin. PIN-FORMED plasma membrane proteins, involved in intercellular transport of the growth regulator, are key to the transmission of such auxin signals and subject to multilevel surveillance mechanisms, including reversible post-translational modifications. Apart from well-studied PIN protein modifications, namely phosphorylation and ubiquitylation, no further post-translational modifications have been described so far. Here, we focused on root-specific Arabidopsis PIN2 and explored functional implications of two evolutionary conserved cysteines, by a combination of in silico and molecular approaches. PIN2 sequence alignments and modeling predictions indicated that both cysteines are facing the cytoplasm and therefore would be accessible to redox status-controlled modifications. Notably, mutant pin2C-A alleles retained functionality, demonstrated by their ability to almost completely rescue defects of a pin2 null allele, whereas high resolution analysis of pin2C-A localization revealed increased intracellular accumulation, and altered protein distribution within plasma membrane micro-domains. The observed effects of cysteine replacements on root growth and PIN2 localization are consistent with a model in which redox status-dependent cysteine modifications participate in the regulation of PIN2 mobility, thereby fine-tuning polar auxin transport.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18024503
003      
CZ-PrNML
005      
20180710095249.0
007      
ta
008      
180709s2017 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/ijms18112274 $2 doi
035    __
$a (PubMed)29109378
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Retzer, Katarzyna $u Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria. retzer@ueb.cas.cz. Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. retzer@ueb.cas.cz.
245    10
$a Evolutionary Conserved Cysteines Function as cis-Acting Regulators of Arabidopsis PIN-FORMED 2 Distribution / $c K. Retzer, J. Lacek, R. Skokan, CI. Del Genio, S. Vosolsobě, M. Laňková, K. Malínská, N. Konstantinova, E. Zažímalová, RM. Napier, J. Petrášek, C. Luschnig,
520    9_
$a Coordination of plant development requires modulation of growth responses that are under control of the phytohormone auxin. PIN-FORMED plasma membrane proteins, involved in intercellular transport of the growth regulator, are key to the transmission of such auxin signals and subject to multilevel surveillance mechanisms, including reversible post-translational modifications. Apart from well-studied PIN protein modifications, namely phosphorylation and ubiquitylation, no further post-translational modifications have been described so far. Here, we focused on root-specific Arabidopsis PIN2 and explored functional implications of two evolutionary conserved cysteines, by a combination of in silico and molecular approaches. PIN2 sequence alignments and modeling predictions indicated that both cysteines are facing the cytoplasm and therefore would be accessible to redox status-controlled modifications. Notably, mutant pin2C-A alleles retained functionality, demonstrated by their ability to almost completely rescue defects of a pin2 null allele, whereas high resolution analysis of pin2C-A localization revealed increased intracellular accumulation, and altered protein distribution within plasma membrane micro-domains. The observed effects of cysteine replacements on root growth and PIN2 localization are consistent with a model in which redox status-dependent cysteine modifications participate in the regulation of PIN2 mobility, thereby fine-tuning polar auxin transport.
650    _2
$a Arabidopsis $x genetika $x metabolismus $7 D017360
650    _2
$a proteiny huseníčku $x chemie $x genetika $x metabolismus $7 D029681
650    12
$a konzervovaná sekvence $7 D017124
650    _2
$a cystein $x genetika $7 D003545
650    _2
$a kyseliny indoloctové $x metabolismus $7 D007210
650    _2
$a membránové mikrodomény $x metabolismus $7 D021962
650    _2
$a kořeny rostlin $x růst a vývoj $x metabolismus $7 D018517
650    _2
$a transport proteinů $7 D021381
655    _2
$a časopisecké články $7 D016428
700    1_
$a Lacek, Jozef $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. lacek@ueb.cas.cz. Department of Experimental Plant Biology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic. lacek@ueb.cas.cz.
700    1_
$a Skokan, Roman $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. skokan@ueb.cas.cz. Department of Experimental Plant Biology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic. skokan@ueb.cas.cz.
700    1_
$a Del Genio, Charo I $u School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK. the.paraw@gmail.com.
700    1_
$a Vosolsobě, Stanislav $u Department of Experimental Plant Biology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic. stanislav.vosolsobe@natur.cuni.cz.
700    1_
$a Laňková, Martina $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. lankova@ueb.cas.cz.
700    1_
$a Malínská, Kateřina $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. malinska@ueb.cas.cz.
700    1_
$a Konstantinova, Nataliia $u Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria. nataliia.konstantinova@boku.ac.at.
700    1_
$a Zažímalová, Eva $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. Zazimalova@ueb.cas.cz.
700    1_
$a Napier, Richard M $u School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry CV4 7AL, UK. Richard.Napier@warwick.ac.uk.
700    1_
$a Petrášek, Jan $u Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 02 Praha 6, Czech Republic. petrasek@ueb.cas.cz. Department of Experimental Plant Biology, Faculty of Science, Charles University, Vinicna 5, 128 44 Prague 2, Czech Republic. petrasek@ueb.cas.cz.
700    1_
$a Luschnig, Christian $u Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Wien, Austria. christian.luschnig@boku.ac.at.
773    0_
$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 18, č. 11 (2017)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29109378 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20180709 $b ABA008
991    __
$a 20180710095539 $b ABA008
999    __
$a ok $b bmc $g 1316634 $s 1021424
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 18 $c 11 $e 20171029 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
LZP    __
$a Pubmed-20180709

Najít záznam

Citační ukazatele

Možnosti archivace