• Je něco špatně v tomto záznamu ?

Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS Analysis

I. Chamrád, J. Uřinovská, B. Petrovská, H. Jeřábková, R. Lenobel, J. Vrána, J. Doležel, M. Šebela,

. 2018 ; 1696 (-) : 57-79.

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc18024519

In the plant nucleus, the majority of cellular DNA content is stored and maintained. This makes this highly specialized organelle the major coordinator of almost all essential processes in plant cells such as transcription, DNA replication, and repair. None of these biological pathways can be fully understood without a comprehensive characterization of nuclear proteins. Nevertheless, the interest of the proteomic community in the plant nuclear proteome has been very limited so far. This is probably due to the high integrity of plant cell, presence of many interfering metabolites, and considerable endogenous proteolytic activity which make the sample preparation problematic. Hereby, we describe a novel protocol for the high-throughput plant nuclear protein identification that combines a flow cytometric sorting of formaldehyde-fixed nuclei with protein and peptide separation and their subsequent LC-MS/MS analysis.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc18024519
003      
CZ-PrNML
005      
20180710092723.0
007      
ta
008      
180709s2018 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1007/978-1-4939-7411-5_4 $2 doi
035    __
$a (PubMed)29086396
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Chamrád, Ivo $u Department of Protein Biochemistry and Proteomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71, Olomouc, Czech Republic. ivo.chamrad@upol.cz.
245    10
$a Identification of Plant Nuclear Proteins Based on a Combination of Flow Sorting, SDS-PAGE, and LC-MS/MS Analysis / $c I. Chamrád, J. Uřinovská, B. Petrovská, H. Jeřábková, R. Lenobel, J. Vrána, J. Doležel, M. Šebela,
520    9_
$a In the plant nucleus, the majority of cellular DNA content is stored and maintained. This makes this highly specialized organelle the major coordinator of almost all essential processes in plant cells such as transcription, DNA replication, and repair. None of these biological pathways can be fully understood without a comprehensive characterization of nuclear proteins. Nevertheless, the interest of the proteomic community in the plant nuclear proteome has been very limited so far. This is probably due to the high integrity of plant cell, presence of many interfering metabolites, and considerable endogenous proteolytic activity which make the sample preparation problematic. Hereby, we describe a novel protocol for the high-throughput plant nuclear protein identification that combines a flow cytometric sorting of formaldehyde-fixed nuclei with protein and peptide separation and their subsequent LC-MS/MS analysis.
650    _2
$a elektroforéza v polyakrylamidovém gelu $7 D004591
650    _2
$a průtoková cytometrie $7 D005434
650    _2
$a plynová chromatografie s hmotnostně spektrometrickou detekcí $7 D008401
650    _2
$a ječmen (rod) $x cytologie $x metabolismus $7 D001467
650    _2
$a jaderné proteiny $x analýza $7 D009687
650    _2
$a rostlinné proteiny $x analýza $7 D010940
650    _2
$a proteomika $x metody $7 D040901
655    _2
$a časopisecké články $7 D016428
700    1_
$a Uřinovská, Jana $u Department of Protein Biochemistry and Proteomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71, Olomouc, Czech Republic.
700    1_
$a Petrovská, Beáta $u Centre of Plant Structural and Functional Genomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR, Šlechtitelů 31, 783 71, Olomouc, Czech Republic.
700    1_
$a Jeřábková, Hana $u Centre of Plant Structural and Functional Genomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR, Šlechtitelů 31, 783 71, Olomouc, Czech Republic.
700    1_
$a Lenobel, René $u Department of Protein Biochemistry and Proteomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71, Olomouc, Czech Republic.
700    1_
$a Vrána, Jan $u Centre of Plant Structural and Functional Genomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR, Šlechtitelů 31, 783 71, Olomouc, Czech Republic.
700    1_
$a Doležel, Jaroslav $u Centre of Plant Structural and Functional Genomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Institute of Experimental Botany AS CR, Šlechtitelů 31, 783 71, Olomouc, Czech Republic.
700    1_
$a Šebela, Marek $u Department of Protein Biochemistry and Proteomics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, 783 71, Olomouc, Czech Republic.
773    0_
$w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 1696, č. - (2018), s. 57-79
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29086396 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20180709 $b ABA008
991    __
$a 20180710093013 $b ABA008
999    __
$a ok $b bmc $g 1316650 $s 1021440
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 1696 $c - $d 57-79 $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
LZP    __
$a Pubmed-20180709

Najít záznam

Citační ukazatele

Pouze přihlášení uživatelé

Možnosti archivace