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Extensive flagellar remodeling during the complex life cycle of Paratrypanosoma, an early-branching trypanosomatid
T. Skalický, E. Dobáková, RJ. Wheeler, M. Tesařová, P. Flegontov, D. Jirsová, J. Votýpka, V. Yurchenko, FJ. Ayala, J. Lukeš,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 1915 to 6 months ago
Freely Accessible Science Journals
from 1915 to 6 months ago
PubMed Central
from 1915 to 6 months ago
Europe PubMed Central
from 1915 to 6 months ago
Open Access Digital Library
from 1915-01-15
Open Access Digital Library
from 1915-01-01
- MeSH
- Cytoskeleton genetics MeSH
- Flagella genetics MeSH
- Phylogeny MeSH
- Genome, Protozoan genetics MeSH
- Leishmania genetics MeSH
- Humans MeSH
- Protozoan Proteins genetics MeSH
- Life Cycle Stages genetics MeSH
- Gene Expression Profiling methods MeSH
- Trypanosoma cruzi genetics MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Paratrypanosoma confusum is a monoxenous kinetoplastid flagellate that constitutes the most basal branch of the highly diverse parasitic trypanosomatids, which include human pathogens Trypanosoma and Leishmania This makes Paratrypanosoma uniquely informative for the evolution of obligatory parasitism from free-living lifestyle and the evolution of human parasitism in some trypanosomatid lineages. It has typical promastigote morphology but also forms surface-attached haptomonads and amastigotes. Haptomonads form by attachment to a surface via a large bulge at the base of the flagellum, which is then remodeled into a thin attachment pad associated with flagellum shortening. Promastigotes and haptomonads multiply by binary division, and the progeny of a haptomonad can either remain attached or grow a flagellum and resume swimming. Whole genome sequencing and transcriptome profiling, in combination with analysis of the cell ultrastructure, reveal how the cell surface and metabolism are adapted to parasitism and how characteristic cytoskeletal features are conserved. Our data demonstrate that surface attachment by the flagellum and the flagellar pocket, a Leishmania-like flagellum attachment zone, and a Trypanosoma cruzi-like cytostome are ancestral features, while evolution of extant trypanosomatids, including the human parasites, is associated with genome streamlining and diversification of membrane proteins.
Department of Ecology and Evolutionary Biology University of California Irvine CA 92697
Faculty of Science University of South Bohemia 37005 České Budějovice Czech Republic
Sir William Dunn School of Pathology University of Oxford Oxford OX1 3RE United Kingdom
References provided by Crossref.org
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