Morbilliviruses, such as Cetacean morbillivirus (CeMV) or Phocine distemper virus (PDV), represent a growing threat for marine mammals on both hemispheres. Because free-ranging animal populations strongly rely on natural resistance mechanisms, innate immunity-related genes and virus cell entry receptor genes may represent key factors involved in susceptibility to CeMV in Cetaceans. Using the next generation sequencing technology, we have sequenced 11 candidate genes in two model species, Stenella coeruleoalba and Phocoena phocoena. Suitable single nucleotide polymorphism markers of potential functional importance, located in genes coding for basigin (BSG, CD147), the signaling lymphocyte activating molecule (SLAMF1), the poliovirus-related receptor-4 (NECTIN4, PVRL4), toll-like receptors 3, 7, 8 (TLR3, TLR7, TLR8), natural resistance-associated macrophage protein (SLC11A1) and natural cytotoxicity triggering receptor 1 (NCR1), were identified in each model species, along with MHC-DQB haplotypes unique for each species. This set of molecular markers represents a potentially useful tool for studying host genetic variation and susceptibility to morbillivirus infection in Cetaceans as well as for studying functionally important genetic diversity of selected Cetacean populations.

Ceitec MU Masaryk University Brno Czech Republic Faculty of Science National Centre for Biomolecular Research Masaryk University Brno Czech Republic

Ceitec VFU RG Animal Immunogenomics Brno Czech Republic

Ceitec VFU RG Molecular Microbiology Brno Czech Republic

Ceitec VFU RG Molecular Microbiology Brno Czech Republic Department of Pathology and Parasitology University of Veterinary and Pharmaceutical Sciences Brno Czech Republic Biology Center Czech Academy of Sciences České Budějovice Czech Republic

Department of Animal Genetics University of Veterinary and Pharmaceutical Sciences Brno Czech Republic

Department of Animal Genetics University of Veterinary and Pharmaceutical Sciences Brno Czech Republic Ceitec VFU RG Animal Immunogenomics Brno Czech Republic

Department of Comparative Biomedicine and Food Science Viale dell'Università University of Padua Padua Italy

Faculty of Veterinary Medicine University of Agricultural Sciences and Veterinary Medicine Cluj Napoca Cluj Napoca Romania

Faculty of Veterinary Medicine University of Teramo Teramo Italy

Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M Aleandri Pisa Italy

Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G Caporale Teramo Italy

Mare Nostrum NGO Constanta Romania

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$a Morbilliviruses, such as Cetacean morbillivirus (CeMV) or Phocine distemper virus (PDV), represent a growing threat for marine mammals on both hemispheres. Because free-ranging animal populations strongly rely on natural resistance mechanisms, innate immunity-related genes and virus cell entry receptor genes may represent key factors involved in susceptibility to CeMV in Cetaceans. Using the next generation sequencing technology, we have sequenced 11 candidate genes in two model species, Stenella coeruleoalba and Phocoena phocoena. Suitable single nucleotide polymorphism markers of potential functional importance, located in genes coding for basigin (BSG, CD147), the signaling lymphocyte activating molecule (SLAMF1), the poliovirus-related receptor-4 (NECTIN4, PVRL4), toll-like receptors 3, 7, 8 (TLR3, TLR7, TLR8), natural resistance-associated macrophage protein (SLC11A1) and natural cytotoxicity triggering receptor 1 (NCR1), were identified in each model species, along with MHC-DQB haplotypes unique for each species. This set of molecular markers represents a potentially useful tool for studying host genetic variation and susceptibility to morbillivirus infection in Cetaceans as well as for studying functionally important genetic diversity of selected Cetacean populations.

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$a Bayerova, Z $u Department of Animal Genetics, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic.

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$a Futas, J $u Department of Animal Genetics, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic. Ceitec VFU, RG Animal Immunogenomics, Brno, Czech Republic.

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