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Isolation and Characterization of CTCs from Patients with Cancer of a Urothelial Origin
V. Bobek, K. Kolostova,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
- MeSH
- buněčné kultury MeSH
- DNA nádorová genetika izolace a purifikace MeSH
- lidé MeSH
- mutační analýza DNA metody MeSH
- nádorové cirkulující buňky metabolismus patologie MeSH
- separace buněk * metody MeSH
- stanovení celkové genové exprese metody MeSH
- urologické nádory diagnóza genetika metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Monitoring of circulating tumor cells' (CTCs) presence has the potential to improve therapeutic management of oncological diseases at an early stage and also to identify patients with increased risk of tumor progression or recurrence before the onset of clinically detected metastasis. Here we describe a new simplified efficient methodology for the separation and in vitro culturing of viable CTCs from peripheral blood by size-based filtration (MetaCell®). The isolation protocol yields preferentially cells bigger than 8 μm enabling further cytomorphological and molecular analysis.
Citace poskytuje Crossref.org
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- $a Bobek, Vladimir $u Department of Laboratory Genetics, University Hospital Kralovske Vinohrady, Srobarova 50, 10034, Prague, Czech Republic. vbobek@centrum.cz.
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- $a Monitoring of circulating tumor cells' (CTCs) presence has the potential to improve therapeutic management of oncological diseases at an early stage and also to identify patients with increased risk of tumor progression or recurrence before the onset of clinically detected metastasis. Here we describe a new simplified efficient methodology for the separation and in vitro culturing of viable CTCs from peripheral blood by size-based filtration (MetaCell®). The isolation protocol yields preferentially cells bigger than 8 μm enabling further cytomorphological and molecular analysis.
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- $a Kolostova, Katarina $u Department of Laboratory Genetics, University Hospital Kralovske Vinohrady, Srobarova 50, 10034, Prague, Czech Republic.
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