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Polyion complex vesicles (PICsomes) from strong copolyelectrolytes. Stability and in vitro studies

U. Kwolek, K. Nakai, A. Pluta, M. Zatorska, D. Wnuk, S. Lasota, J. Bednar, M. Michalik, SI. Yusa, M. Kepczynski,

. 2017 ; 158 (-) : 658-666. [pub] 20170719

Language English Country Netherlands

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc18024773

Polymer vesicles formed by a pair of oppositely charged diblock copolyelectrolytes (PICsomes) are considered as a good alternative to polymersomes formed by amphiphilic copolymers. Here, we report on inherent stability and in vitro biocompatibility of PICsomes prepared from a pair of oppositely charged zwitterionic-ionic copolymers, in which the ionic block is a strong polyelectrolyte. Our results demonstrated that the PICsomes are highly stable over a wide range of pH and temperatures. Direct microscopic observations revealed that the PICsomes retain their morphology in the presence of human serum. In vitro studies using human skin fibroblasts (HSFs) showed that the polymer vesicles are not cytotoxic and do not affect cell proliferation and adhesion. A model hydrophilic dye was effectively incorporated into the PICsomes by simple mixing. Using confocal microscopy observations, we demonstrated that the dye-loaded PICsomes are efficiently internalized by the cells and are located predominantly in endo/lysosomal compartments. Thus, the PICsomes have promising potential for use as nanocontainers for substances of biomedical interest.

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$a Kwolek, Urszula $u Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
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$a Polymer vesicles formed by a pair of oppositely charged diblock copolyelectrolytes (PICsomes) are considered as a good alternative to polymersomes formed by amphiphilic copolymers. Here, we report on inherent stability and in vitro biocompatibility of PICsomes prepared from a pair of oppositely charged zwitterionic-ionic copolymers, in which the ionic block is a strong polyelectrolyte. Our results demonstrated that the PICsomes are highly stable over a wide range of pH and temperatures. Direct microscopic observations revealed that the PICsomes retain their morphology in the presence of human serum. In vitro studies using human skin fibroblasts (HSFs) showed that the polymer vesicles are not cytotoxic and do not affect cell proliferation and adhesion. A model hydrophilic dye was effectively incorporated into the PICsomes by simple mixing. Using confocal microscopy observations, we demonstrated that the dye-loaded PICsomes are efficiently internalized by the cells and are located predominantly in endo/lysosomal compartments. Thus, the PICsomes have promising potential for use as nanocontainers for substances of biomedical interest.
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$a Nakai, Keita $u Department of Applied Chemistry, University of Hyogo 2167 Shosha, Himeji, Hyogo 671-2280, Japan.
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$a Pluta, Anna $u Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
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$a Zatorska, Maria $u Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland.
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$a Wnuk, Dawid $u Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
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$a Lasota, Sławomir $u Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
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$a Bednar, Jan $u Université de Grenoble Alpes/CNRS, Institut Albert Bonniot, UMR 5309, 38042 Grenoble Cedex 9, France; Charles University in Prague, 1st Faculty of Medicine, Laboratory of Biology and Pathology of the Eye, Institute of Inherited Metabolic Disorders, KeKarlovu 2, 12800 Prague 2, Czech Republic.
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$a Michalik, Marta $u Department of Cell Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
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$a Yusa, Shin-Ichi $u Department of Applied Chemistry, University of Hyogo 2167 Shosha, Himeji, Hyogo 671-2280, Japan. Electronic address: yusa@eng.u-hyogo.ac.jp.
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$a Kepczynski, Mariusz $u Faculty of Chemistry, Jagiellonian University, Ingardena 3, 30-060 Kraków, Poland. Electronic address: kepczyns@chemia.uj.edu.pl.
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