Poly(lactic acid) (PLA) has shown much success in the preparation of tissue engineering scaffolds as it can be fabricated with a tailored architecture. However, the PLA surface has drawbacks including the lack of biofunctional motifs which are essential for high affinity to biological cells. Therefore, this study describes a multistep physicochemical approach for the immobilization of d-glucosamine (GlcN), a naturally occurring monosaccharide having many biological functions, on the PLA surface aiming at enhancing the cell proliferation activity. In this approach, poly(acrylic acid) (PAAc) spacer arms are first introduced into the PLA surface via plasma post-irradiation grafting technique. Then, covalent coupling or physical adsorption of GlcN with/on the PAAc spacer is carried out. Factors affecting the grafting yield are controlled to produce a suitable spacer for bioimmobilization. X-ray photon spectroscopic (XPS) analyses confirm the immobilization of GlcN on the PLA surface. The XPS results reveal also that increasing the yield of grafted PAAc spacer on the PLA surface increases the amount of covalently immobilized GlcN, but actually inhibits the immobilization process using the physical adsorption method. Contact angle measurements and atomic force microscopy (AFM) show a substantial increase of surface energy and roughness of PLA surface, respectively, upon the multistep modification procedure. The cytocompatibility of the modified surfaces is assessed using a mouse embryonic fibroblast (MEF) cell line. Observation from the cell culture basically demonstrates the potential of GlcN immobilization in improving the cytocompatibility of the PLA surface. Moreover, the covalent immobilization of GlcN seems to produce more cytocompatible surfaces if compared with the physical adsorption method. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3176-3188, 2017.

Centre of Polymer Systems Tomas Bata University in Zlín Trida Tomase Bati 5678 Zlín 760 01 Czech Republic

Centre of Polymer Systems Tomas Bata University in Zlín Trida Tomase Bati 5678 Zlín 760 01 Czech Republic Department of Chemistry Faculty of Science Ain Shams University Abbassia Cairo 11566 Egypt

Department of Chemistry Faculty of Science Ain Shams University Abbassia Cairo 11566 Egypt

Department of Polymers National Center for Radiation Research and Technology Atomic Energy Authority Nasr City Cairo 11371 Egypt

Faculty of Mathematics and Physics Charles University Prague 5 Holesovickach 2 Prague 8 18000 Czech Republic

Polymer Institute Slovak Academy of Sciences Dúbravská cesta 9 Bratislava Slovakia 845

000      

00000naa a2200000 a 4500

001      

bmc18024841

003      

CZ-PrNML

005      

20180717082105.0

007      

ta

008      

180709s2017 xxu f 000 0|eng||

009      

AR

024    7_

$a 10.1002/jbm.a.36158 $2 doi

035    __

$a (PubMed)28707422

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a xxu

100    1_

$a Swilem, Ahmed E $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, Zlín, 760 01, Czech Republic. Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo, 11566, Egypt.

245    10

$a Description of D-glucosamine immobilization kinetics onto poly(lactic acid) surface via a multistep physicochemical approach for preparation of novel active biomaterials / $c AE. Swilem, M. Lehocký, P. Humpolíček, Z. Kucekova, I. Novák, M. Mičušík, HA. Abd El-Rehim, EA. Hegazy, AA. Hamed, J. Kousal,

520    9_

$a Poly(lactic acid) (PLA) has shown much success in the preparation of tissue engineering scaffolds as it can be fabricated with a tailored architecture. However, the PLA surface has drawbacks including the lack of biofunctional motifs which are essential for high affinity to biological cells. Therefore, this study describes a multistep physicochemical approach for the immobilization of d-glucosamine (GlcN), a naturally occurring monosaccharide having many biological functions, on the PLA surface aiming at enhancing the cell proliferation activity. In this approach, poly(acrylic acid) (PAAc) spacer arms are first introduced into the PLA surface via plasma post-irradiation grafting technique. Then, covalent coupling or physical adsorption of GlcN with/on the PAAc spacer is carried out. Factors affecting the grafting yield are controlled to produce a suitable spacer for bioimmobilization. X-ray photon spectroscopic (XPS) analyses confirm the immobilization of GlcN on the PLA surface. The XPS results reveal also that increasing the yield of grafted PAAc spacer on the PLA surface increases the amount of covalently immobilized GlcN, but actually inhibits the immobilization process using the physical adsorption method. Contact angle measurements and atomic force microscopy (AFM) show a substantial increase of surface energy and roughness of PLA surface, respectively, upon the multistep modification procedure. The cytocompatibility of the modified surfaces is assessed using a mouse embryonic fibroblast (MEF) cell line. Observation from the cell culture basically demonstrates the potential of GlcN immobilization in improving the cytocompatibility of the PLA surface. Moreover, the covalent immobilization of GlcN seems to produce more cytocompatible surfaces if compared with the physical adsorption method. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3176-3188, 2017.

650    _2

$a adsorpce $7 D000327

650    _2

$a zvířata $7 D000818

650    _2

$a biokompatibilní materiály $x chemie $7 D001672

650    _2

$a buněčné linie $7 D002460

650    _2

$a proliferace buněk $7 D049109

650    _2

$a fibroblasty $x cytologie $7 D005347

650    _2

$a glukosamin $x chemie $7 D005944

650    _2

$a kinetika $7 D007700

650    _2

$a myši $7 D051379

650    _2

$a mikroskopie atomárních sil $7 D018625

650    _2

$a polyestery $x chemie $7 D011091

650    _2

$a povrchové vlastnosti $7 D013499

650    _2

$a tkáňové podpůrné struktury $x chemie $7 D054457

655    _2

$a časopisecké články $7 D016428

700    1_

$a Lehocký, Marian $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, Zlín, 760 01, Czech Republic.

700    1_

$a Humpolíček, Petr $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, Zlín, 760 01, Czech Republic.

700    1_

$a Kucekova, Zdenka $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, Zlín, 760 01, Czech Republic.

700    1_

$a Novák, Igor $u Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, Slovakia, 845 41.

700    1_

$a Mičušík, Matej $u Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, Bratislava, Slovakia, 845 41.

700    1_

$a Abd El-Rehim, Hassan A $u Department of Polymers, National Center for Radiation Research and Technology, Atomic Energy Authority, Nasr City, Cairo, 11371, Egypt.

700    1_

$a Hegazy, El-Sayed A $u Department of Polymers, National Center for Radiation Research and Technology, Atomic Energy Authority, Nasr City, Cairo, 11371, Egypt.

700    1_

$a Hamed, Ashraf A $u Department of Chemistry, Faculty of Science, Ain Shams University, Abbassia, Cairo, 11566, Egypt.

700    1_

$a Kousal, Jaroslav $u Faculty of Mathematics and Physics, Charles University Prague, V Holesovickach 2, Prague 8, 18000, Czech Republic.

773    0_

$w MED00007498 $t Journal of biomedical materials research. Part A $x 1552-4965 $g Roč. 105, č. 11 (2017), s. 3176-3188

856    41

$u https://pubmed.ncbi.nlm.nih.gov/28707422 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20180709 $b ABA008

991    __

$a 20180717082404 $b ABA008

999    __

$a ok $b bmc $g 1316972 $s 1021762

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2017 $b 105 $c 11 $d 3176-3188 $e 20170915 $i 1552-4965 $m Journal of biomedical materials research. Part A $n J Biomed Mater Res $x MED00007498

LZP    __

$a Pubmed-20180709