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Two flagellar BAR domain proteins in Trypanosoma brucei with stage-specific regulation

Z. Cicova, M. Dejung, T. Skalicky, N. Eisenhuth, S. Hanselmann, B. Morriswood, LM. Figueiredo, F. Butter, CJ. Janzen,

. 2016 ; 6 (-) : 35826. [pub] 20161025

Language English Country England, Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc18025282

Trypanosomes are masters of adaptation to different host environments during their complex life cycle. Large-scale proteomic approaches provide information on changes at the cellular level, and in a systematic way. However, detailed work on single components is necessary to understand the adaptation mechanisms on a molecular level. Here, we have performed a detailed characterization of a bloodstream form (BSF) stage-specific putative flagellar host adaptation factor Tb927.11.2400, identified previously in a SILAC-based comparative proteome study. Tb927.11.2400 shares 38% amino acid identity with TbFlabarin (Tb927.11.2410), a procyclic form (PCF) stage-specific flagellar BAR domain protein. We named Tb927.11.2400 TbFlabarin-like (TbFlabarinL), and demonstrate that it originates from a gene duplication event, which occurred in the African trypanosomes. TbFlabarinL is not essential for the growth of the parasites under cell culture conditions and it is dispensable for developmental differentiation from BSF to the PCF in vitro. We generated TbFlabarinL-specific antibodies, and showed that it localizes in the flagellum. Co-immunoprecipitation experiments together with a biochemical cell fractionation suggest a dual association of TbFlabarinL with the flagellar membrane and the components of the paraflagellar rod.

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$a Dejung, Mario $u Institute of Molecular Biology (IMB), Mainz, Germany.
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$a Skalicky, Tomas $u Laboratory of Molecular Biology of Protists, Institute of Parasitology Biology Centre, Czech Academy of Science, and Faculty of Sciences, University of South Bohemia Ceske Budejovice, Czech Republic.
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$a Eisenhuth, Nicole $u Department of Cell &Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany.
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$a Hanselmann, Steffen $u Department of Cell &Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany.
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$a Morriswood, Brooke $u Department of Cell &Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany.
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$a Figueiredo, Luisa M $u Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa 1649-028, Portugal.
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$a Butter, Falk $u Institute of Molecular Biology (IMB), Mainz, Germany.
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$a Janzen, Christian J $u Department of Cell &Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany.
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