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Současné metody hodnocení oseointegrace implantátů z nanostrukturního titanu
Amin Moztarzadeh, Omid Moztarzadeh, Tereza Kubíková, Zbyněk Tonar, Daniel Hrušák, Antonín Zicha, Václav Babuška
Jazyk čeština Země Česko
Typ dokumentu práce podpořená grantem
Grantová podpora
NV15-25813A
MZ0
CEP - Centrální evidence projektů
Digitální knihovna NLK
Plný text - Článek
Zdroj
E-zdroje NLK Online
ROAD: Directory of Open Access Scholarly Resources od 1997
Odkazy
domovská stránka časopisu
- MeSH
- lidé MeSH
- nanostruktury MeSH
- titan MeSH
- zubní implantáty * MeSH
- zubní materiály MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Currently, titanium endosseous implants represent the standard in dental implantology. To improve their mechanical properties, biocompatibility and osseointegration, the concept of bulk nanostructured titanium with variously textured surface is being developed. Different animal species, mostly pigs, minipigs and rabbits, are used for the osseointegration assessment. The selection of implantation site in the target bone is also important, because various types of bones have different ratio between the compact and the trabecular bone tissue. After finishing the in vivo experiment, the implant position and orientation is verified by X-ray imaging. The part of the bone with the implant is surgically removed, cut into tissue blocks, and histologically processed using technique of undemineralized ground sections. This includes embedding the samples into resin followed by sawing, grinding, and polishing of transparent histological sections. A subsequent staining provides information on the formation and mineralization of the bone matrix in close proximity of the implant. Osseointegration is evaluated using a bone-implant contact (BIC) expressed as the ratio of the implant length in contact with bone tissue and total length of the implant. Additional information about the newly formed bone in regions surrounding the implant may be also provided by a double tetracycline test. According to our own experiments, this methodology is simple to perform, highly reproducible, and gives consistent results.
Stomatologická klinika Lékařská fakulta v Plzni Univerzita Karlova a Fakultní nemocnice Plzeň Plzeň
Ústav anatomie Lékařská fakulta v Plzni Univerzita Karlova Plzeň
Ústav lékařské chemie a biochemie Lékařská fakulta v Plzni Univerzita Karlova Plzeň
Literatura
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- $a Currently, titanium endosseous implants represent the standard in dental implantology. To improve their mechanical properties, biocompatibility and osseointegration, the concept of bulk nanostructured titanium with variously textured surface is being developed. Different animal species, mostly pigs, minipigs and rabbits, are used for the osseointegration assessment. The selection of implantation site in the target bone is also important, because various types of bones have different ratio between the compact and the trabecular bone tissue. After finishing the in vivo experiment, the implant position and orientation is verified by X-ray imaging. The part of the bone with the implant is surgically removed, cut into tissue blocks, and histologically processed using technique of undemineralized ground sections. This includes embedding the samples into resin followed by sawing, grinding, and polishing of transparent histological sections. A subsequent staining provides information on the formation and mineralization of the bone matrix in close proximity of the implant. Osseointegration is evaluated using a bone-implant contact (BIC) expressed as the ratio of the implant length in contact with bone tissue and total length of the implant. Additional information about the newly formed bone in regions surrounding the implant may be also provided by a double tetracycline test. According to our own experiments, this methodology is simple to perform, highly reproducible, and gives consistent results.
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