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Preterm prelabor rupture of membranes (PPROM) is not associated with presence of viral genomes in the amniotic fluid
S Bopegamage, M Kacerovsky, V Tambor, I Musilova, S Sarmirova, E Snelders, Jong AS de, SG Vari, WJ Melchers, JM Galama
Jazyk angličtina Země Nizozemsko
Grantová podpora
NT14104
MZ0
CEP - Centrální evidence projektů
- MeSH
- dospělí MeSH
- genom virový MeSH
- lidé MeSH
- mladý dospělý MeSH
- plodová voda * virologie MeSH
- polymerázová řetězová reakce MeSH
- předčasný odtok plodové vody * etiologie MeSH
- viry genetika izolace a purifikace MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- ženské pohlaví MeSH
BACKGROUND: The role of viral infections in preterm prelabor rupture of the membranes (PPROM) is not established. Studies on the presence of viral genomes in the amniotic fluid (AF) collected in pregnancies complicated by PPROM show contradictory outcomes. OBJECTIVES: To investigate AF samples of PPROM pregnancies for the presence of viral genomes. STUDY DESIGN: AF samples from patients with PPROM were collected during a 4-year (2008-2012) observational study. 174 women were included with selection criteria of singleton pregnancy, PPROM, and maternal age of 18 years and above. PCR was used for detection of human cytomegalovirus (HCMV), herpes simplex virus (HSV), parvovirus B19, human adenoviruses (HAdV), enteroviruses (EV) and human parechovirus (HPeV). The selection of these viral targets was based on literature regarding screening of AF for presence of viral genomes. RESULTS: Only a single sample was positive out of the 174 tested AFs, HCMV DNA was detected. CONCLUSIONS: PPROM is not associated with active viral infections.
Citace poskytuje Crossref.org
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- $a Bopegamage S $u Bopegamage, Shubhada. Enterovirus Laboratory, Medical Faculty, Slovak Medical University, Limbova 12, 83303 Bratislava, Slovak Republic. Electronic address: shubhada.bopegamage@szu.sk.
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- $a BACKGROUND: The role of viral infections in preterm prelabor rupture of the membranes (PPROM) is not established. Studies on the presence of viral genomes in the amniotic fluid (AF) collected in pregnancies complicated by PPROM show contradictory outcomes. OBJECTIVES: To investigate AF samples of PPROM pregnancies for the presence of viral genomes. STUDY DESIGN: AF samples from patients with PPROM were collected during a 4-year (2008-2012) observational study. 174 women were included with selection criteria of singleton pregnancy, PPROM, and maternal age of 18 years and above. PCR was used for detection of human cytomegalovirus (HCMV), herpes simplex virus (HSV), parvovirus B19, human adenoviruses (HAdV), enteroviruses (EV) and human parechovirus (HPeV). The selection of these viral targets was based on literature regarding screening of AF for presence of viral genomes. RESULTS: Only a single sample was positive out of the 174 tested AFs, HCMV DNA was detected. CONCLUSIONS: PPROM is not associated with active viral infections.<ovid:br /><ovid:br />Copyright © 2013 Elsevier B.V. All rights reserved.
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