• Je něco špatně v tomto záznamu ?

Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping

H. Buchtelova, V. Strmiska, Z. Skubalova, S. Dostalova, P. Michalek, S. Krizkova, D. Hynek, L. Kalina, L. Richtera, A. Moulick, V. Adam, Z. Heger,

. 2018 ; 16 (1) : 43. [pub] 20180419

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19000794

BACKGROUND: Suitable fluorophores are the core of fluorescence imaging. Among the most exciting, yet controversial, labels are quantum dots (QDs) with their unique optical and chemical properties, but also considerable toxicity. This hinders QDs applicability in living systems. Surface chemistry has a profound impact on biological behavior of QDs. This study describes a two-step synthesis of QDs formed by CdTe core doped with Schiff base ligand for lanthanides [Ln (Yb3+, Tb3+ and Gd3+)] as novel cytocompatible fluorophores. RESULTS: Microwave-assisted synthesis resulted in water-soluble nanocrystals with high colloidal and fluorescence stability with quantum yields of 40.9-58.0%. Despite induction of endocytosis and cytoplasm accumulation of Yb- and TbQDs, surface doping resulted in significant enhancement in cytocompatibility when compared to the un-doped CdTe QDs. Furthermore, only negligible antimigratory properties without triggering formation of reactive oxygen species were found, particularly for TbQDs. Ln-doped QDs did not cause observable hemolysis, adsorbed only a low degree of plasma proteins onto their surface and did not possess significant genotoxicity. To validate the applicability of Ln-doped QDs for in vitro visualization of receptor status of living cells, we performed a site-directed conjugation of antibodies towards immuno-labeling of clinically relevant target-human norepinephrine transporter (hNET), over-expressed in neuroendocrine tumors like neuroblastoma. Immuno-performance of modified TbQDs was successfully tested in distinct types of cells varying in hNET expression and also in neuroblastoma cells with hNET expression up-regulated by vorinostat. CONCLUSION: For the first time we show that Ln-doping of CdTe QDs can significantly alleviate their cytotoxic effects. The obtained results imply great potential of Ln-doped QDs as cytocompatible and stable fluorophores for various bio-labeling applications.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19000794
003      
CZ-PrNML
005      
20190111150224.0
007      
ta
008      
190107s2018 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1186/s12951-018-0369-7 $2 doi
035    __
$a (PubMed)29673366
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Buchtelova, Hana $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.
245    10
$a Improving cytocompatibility of CdTe quantum dots by Schiff-base-coordinated lanthanides surface doping / $c H. Buchtelova, V. Strmiska, Z. Skubalova, S. Dostalova, P. Michalek, S. Krizkova, D. Hynek, L. Kalina, L. Richtera, A. Moulick, V. Adam, Z. Heger,
520    9_
$a BACKGROUND: Suitable fluorophores are the core of fluorescence imaging. Among the most exciting, yet controversial, labels are quantum dots (QDs) with their unique optical and chemical properties, but also considerable toxicity. This hinders QDs applicability in living systems. Surface chemistry has a profound impact on biological behavior of QDs. This study describes a two-step synthesis of QDs formed by CdTe core doped with Schiff base ligand for lanthanides [Ln (Yb3+, Tb3+ and Gd3+)] as novel cytocompatible fluorophores. RESULTS: Microwave-assisted synthesis resulted in water-soluble nanocrystals with high colloidal and fluorescence stability with quantum yields of 40.9-58.0%. Despite induction of endocytosis and cytoplasm accumulation of Yb- and TbQDs, surface doping resulted in significant enhancement in cytocompatibility when compared to the un-doped CdTe QDs. Furthermore, only negligible antimigratory properties without triggering formation of reactive oxygen species were found, particularly for TbQDs. Ln-doped QDs did not cause observable hemolysis, adsorbed only a low degree of plasma proteins onto their surface and did not possess significant genotoxicity. To validate the applicability of Ln-doped QDs for in vitro visualization of receptor status of living cells, we performed a site-directed conjugation of antibodies towards immuno-labeling of clinically relevant target-human norepinephrine transporter (hNET), over-expressed in neuroendocrine tumors like neuroblastoma. Immuno-performance of modified TbQDs was successfully tested in distinct types of cells varying in hNET expression and also in neuroblastoma cells with hNET expression up-regulated by vorinostat. CONCLUSION: For the first time we show that Ln-doping of CdTe QDs can significantly alleviate their cytotoxic effects. The obtained results imply great potential of Ln-doped QDs as cytocompatible and stable fluorophores for various bio-labeling applications.
650    _2
$a sloučeniny kadmia $x toxicita $7 D019187
650    _2
$a nádorové buněčné linie $7 D045744
650    _2
$a fluorescenční barviva $x toxicita $7 D005456
650    _2
$a lidé $7 D006801
650    _2
$a lanthanoidy $x chemie $7 D028581
650    _2
$a mikrovlny $7 D008872
650    _2
$a optické zobrazování $x metody $7 D061848
650    _2
$a kvantové tečky $x toxicita $7 D045663
650    _2
$a Schiffovy báze $x chemie $7 D012545
650    _2
$a analýza jednotlivých buněk $x metody $7 D059010
650    _2
$a povrchové vlastnosti $7 D013499
650    _2
$a telur $x toxicita $7 D013691
655    _2
$a časopisecké články $7 D016428
700    1_
$a Strmiska, Vladislav $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.
700    1_
$a Skubalova, Zuzana $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic.
700    1_
$a Dostalova, Simona $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic.
700    1_
$a Michalek, Petr $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic.
700    1_
$a Krizkova, Sona $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic.
700    1_
$a Hynek, David $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic.
700    1_
$a Kalina, Lukas $u Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic.
700    1_
$a Richtera, Lukas $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic.
700    1_
$a Moulick, Amitava $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic.
700    1_
$a Adam, Vojtech $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic.
700    1_
$a Heger, Zbynek $u Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, 613 00, Brno, Czech Republic. heger@mendelu.cz. Central European Institute of Technology, Brno University of Technology, Purkynova 123, 612 00, Brno, Czech Republic. heger@mendelu.cz.
773    0_
$w MED00008237 $t Journal of nanobiotechnology $x 1477-3155 $g Roč. 16, č. 1 (2018), s. 43
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29673366 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190107 $b ABA008
991    __
$a 20190111150429 $b ABA008
999    __
$a ok $b bmc $g 1364798 $s 1038917
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 16 $c 1 $d 43 $e 20180419 $i 1477-3155 $m Journal of nanobiotechnology $n J Nanobiotechnology $x MED00008237
LZP    __
$a Pubmed-20190107

Najít záznam

Citační ukazatele

Možnosti archivace