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The intracellular distribution of inorganic carbon fixing enzymes does not support the presence of a C4 pathway in the diatom Phaeodactylum tricornutum
D. Ewe, M. Tachibana, S. Kikutani, A. Gruber, C. Río Bártulos, G. Konert, A. Kaplan, Y. Matsuda, PG. Kroth,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
NLK
ProQuest Central
od 1997-01-01 do Před 1 rokem
Medline Complete (EBSCOhost)
od 2011-01-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 1997-01-01 do Před 1 rokem
- MeSH
- Arabidopsis fyziologie MeSH
- fosfoenolpyruvátkarboxylasa klasifikace metabolismus MeSH
- fotosyntéza fyziologie MeSH
- koloběh uhlíku MeSH
- kukuřice setá fyziologie MeSH
- mitochondrie enzymologie MeSH
- pyruvátkarboxylasa genetika metabolismus MeSH
- regulace genové exprese enzymů fyziologie MeSH
- regulace genové exprese u rostlin fyziologie MeSH
- rozsivky enzymologie fyziologie MeSH
- Publikační typ
- časopisecké články MeSH
Diatoms are unicellular algae and important primary producers. The process of carbon fixation in diatoms is very efficient even though the availability of dissolved CO2 in sea water is very low. The operation of a carbon concentrating mechanism (CCM) also makes the more abundant bicarbonate accessible for photosynthetic carbon fixation. Diatoms possess carbonic anhydrases as well as metabolic enzymes potentially involved in C4 pathways; however, the question as to whether a C4 pathway plays a general role in diatoms is not yet solved. While genome analyses indicate that the diatom Phaeodactylum tricornutum possesses all the enzymes required to operate a C4 pathway, silencing of the pyruvate orthophosphate dikinase (PPDK) in a genetically transformed cell line does not lead to reduced photosynthetic carbon fixation. In this study, we have determined the intracellular location of all enzymes potentially involved in C4-like carbon fixing pathways in P. tricornutum by expression of the respective proteins fused to green fluorescent protein (GFP), followed by fluorescence microscopy. Furthermore, we compared the results to known pathways and locations of enzymes in higher plants performing C3 or C4 photosynthesis. This approach revealed that the intracellular distribution of the investigated enzymes is quite different from the one observed in higher plants. In particular, the apparent lack of a plastidic decarboxylase in P. tricornutum indicates that this diatom does not perform a C4-like CCM.
Centre Algatech Institute of Microbiology of the Czech Academy of Sciences Třeboň Czech Republic
Fachbereich Biologie Universität Konstanz 78457 Konstanz Germany
Citace poskytuje Crossref.org
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- $a Ewe, Daniela $u Fachbereich Biologie, Universität Konstanz, 78457, Konstanz, Germany. ewe@alga.cz. Centre Algatech, Institute of Microbiology of the Czech Academy of Sciences, Třeboň, Czech Republic. ewe@alga.cz.
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- $a The intracellular distribution of inorganic carbon fixing enzymes does not support the presence of a C4 pathway in the diatom Phaeodactylum tricornutum / $c D. Ewe, M. Tachibana, S. Kikutani, A. Gruber, C. Río Bártulos, G. Konert, A. Kaplan, Y. Matsuda, PG. Kroth,
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- $a Diatoms are unicellular algae and important primary producers. The process of carbon fixation in diatoms is very efficient even though the availability of dissolved CO2 in sea water is very low. The operation of a carbon concentrating mechanism (CCM) also makes the more abundant bicarbonate accessible for photosynthetic carbon fixation. Diatoms possess carbonic anhydrases as well as metabolic enzymes potentially involved in C4 pathways; however, the question as to whether a C4 pathway plays a general role in diatoms is not yet solved. While genome analyses indicate that the diatom Phaeodactylum tricornutum possesses all the enzymes required to operate a C4 pathway, silencing of the pyruvate orthophosphate dikinase (PPDK) in a genetically transformed cell line does not lead to reduced photosynthetic carbon fixation. In this study, we have determined the intracellular location of all enzymes potentially involved in C4-like carbon fixing pathways in P. tricornutum by expression of the respective proteins fused to green fluorescent protein (GFP), followed by fluorescence microscopy. Furthermore, we compared the results to known pathways and locations of enzymes in higher plants performing C3 or C4 photosynthesis. This approach revealed that the intracellular distribution of the investigated enzymes is quite different from the one observed in higher plants. In particular, the apparent lack of a plastidic decarboxylase in P. tricornutum indicates that this diatom does not perform a C4-like CCM.
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- $a Tachibana, Masaaki $u Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo, 669-1337, Japan. Lion Corporation Pharmaceutical Laboratories No.1, Odawara, Kanagawa, 256-0811, Japan.
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- $a Kikutani, Sae $u Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, Sanda, Hyogo, 669-1337, Japan. Tech Manage Corp., Tokyo, 160-0023, Japan.
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