Exposure to solar radiation is a major cause of environmental human skin damage. The main constituent of solar UV light is UVA radiation (320-400 nm); however, the need for protection against UVA has been marginalized for a long time. As a result, there is still a lack of useful agents for UVA protection. In this study, the effect of silymarin (SM) and its main constituent silybin (SB) pre-treatment on UVA-stimulated damage to primary human dermal fibroblasts were carried out. The cells were pre-treated for 1 h with SB or SM and then were exposed to UVA light, using a solar simulator. The effect of SB and SM on reactive oxygen species (ROS) and glutathione (GSH) level, caspase-3 activity, single-strand breaks (SSB) formation and protein level of matrix metalloproteinase-1 (MMP-1), heme oxygenase-1 (HO-1), and heat shock protein (HSP70) was evaluated. Treatment with both SM and SB resulted in a reduction in UVA-stimulated ROS generation and SSB production, as well as in the prevention of GSH depletion, a decrease in the activation of caspase-3 and protein level of MMP-1. They also moderately increased HO-1 level and reduced HSP70 level. Our data showed that both SM and SB are non-phototoxic and have UVA-photoprotective potential and could be useful agents for UV-protective dermatological preparations.

Department of Medical Chemistry and Biochemistry Faculty of Medicine and Dentistry Palacký University Hněvotínská 3 775 15 Olomouc Czech Republic

Department of Plastic and Aesthetic Surgery University Hospital Olomouc 1 P Pavlova 6 779 00 Olomouc Czech Republic

000      

00000naa a2200000 a 4500

001      

bmc19000857

003      

CZ-PrNML

005      

20211221133502.0

007      

ta

008      

190107s2018 gw f 000 0|eng||

009      

AR

024    7_

$a 10.1007/s00403-018-1828-6 $2 doi

035    __

$a (PubMed)29564550

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a gw

100    1_

$a Rajnochová Svobodová, Alena $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.

245    10

$a UVA-photoprotective potential of silymarin and silybin / $c A. Rajnochová Svobodová, E. Gabrielová, L. Michaelides, P. Kosina, A. Ryšavá, J. Ulrichová, B. Zálešák, J. Vostálová,

520    9_

$a Exposure to solar radiation is a major cause of environmental human skin damage. The main constituent of solar UV light is UVA radiation (320-400 nm); however, the need for protection against UVA has been marginalized for a long time. As a result, there is still a lack of useful agents for UVA protection. In this study, the effect of silymarin (SM) and its main constituent silybin (SB) pre-treatment on UVA-stimulated damage to primary human dermal fibroblasts were carried out. The cells were pre-treated for 1 h with SB or SM and then were exposed to UVA light, using a solar simulator. The effect of SB and SM on reactive oxygen species (ROS) and glutathione (GSH) level, caspase-3 activity, single-strand breaks (SSB) formation and protein level of matrix metalloproteinase-1 (MMP-1), heme oxygenase-1 (HO-1), and heat shock protein (HSP70) was evaluated. Treatment with both SM and SB resulted in a reduction in UVA-stimulated ROS generation and SSB production, as well as in the prevention of GSH depletion, a decrease in the activation of caspase-3 and protein level of MMP-1. They also moderately increased HO-1 level and reduced HSP70 level. Our data showed that both SM and SB are non-phototoxic and have UVA-photoprotective potential and could be useful agents for UV-protective dermatological preparations.

650    _2

$a kaspasa 3 $x metabolismus $7 D053148

650    _2

$a kultivované buňky $7 D002478

650    _2

$a poškození DNA $7 D004249

650    _2

$a fibroblasty $x účinky léků $x patologie $x účinky záření $7 D005347

650    _2

$a glutathion $x metabolismus $7 D005978

650    _2

$a proteiny tepelného šoku HSP70 $x metabolismus $7 D018840

650    _2

$a hemoxygenasa-1 $x metabolismus $7 D051547

650    _2

$a lidé $7 D006801

650    _2

$a matrixová metaloproteinasa 1 $x metabolismus $7 D020781

650    _2

$a primární buněčná kultura $7 D061251

650    _2

$a radiační poranění $x farmakoterapie $7 D011832

650    _2

$a reaktivní formy kyslíku $x metabolismus $7 D017382

650    _2

$a silibinin $7 D000077385

650    _2

$a silymarin $x terapeutické užití $7 D012838

650    _2

$a kůže $x patologie $x účinky záření $7 D012867

650    _2

$a sluneční záření $7 D013472

650    _2

$a ultrafialové záření $x škodlivé účinky $7 D014466

655    _2

$a časopisecké články $7 D016428

700    1_

$a Gabrielová, Eva $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic. $7 xx0267682

700    1_

$a Michaelides, Loizos $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.

700    1_

$a Kosina, Pavel $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.

700    1_

$a Ryšavá, Alena $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.

700    1_

$a Ulrichová, Jitka $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic.

700    1_

$a Zálešák, Bohumil $u Department of Plastic and Aesthetic Surgery, University Hospital Olomouc, I. P. Pavlova 6, 779 00, Olomouc, Czech Republic.

700    1_

$a Vostálová, Jitka $u Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacký University, Hněvotínská 3, 775 15, Olomouc, Czech Republic. j.psotova@email.cz.

773    0_

$w MED00009242 $t Archives of dermatological research $x 1432-069X $g Roč. 310, č. 5 (2018), s. 413-424

856    41

$u https://pubmed.ncbi.nlm.nih.gov/29564550 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20190107 $b ABA008

991    __

$a 20211221133457 $b ABA008

999    __

$a ok $b bmc $g 1364849 $s 1038980

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2018 $b 310 $c 5 $d 413-424 $e 20180321 $i 1432-069X $m Archives of dermatological research $n Arch Dermatol Res $x MED00009242

LZP    __

$a Pubmed-20190107