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Ruthenium dendrimers as carriers for anticancer siRNA
S. Michlewska, M. Ionov, M. Maroto-Díaz, A. Szwed, A. Ihnatsyeu-Kachan, S. Loznikova, D. Shcharbin, M. Maly, RG. Ramirez, FJ. de la Mata, M. Bryszewska,
Language English Country United States
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Leukemia, Promyelocytic, Acute drug therapy metabolism pathology MeSH
- Circular Dichroism MeSH
- Dendrimers administration & dosage chemistry metabolism MeSH
- Dynamic Light Scattering MeSH
- Absorption, Physiological MeSH
- HL-60 Cells MeSH
- Intercalating Agents administration & dosage chemistry metabolism MeSH
- Humans MeSH
- RNA, Small Interfering administration & dosage chemistry metabolism ultrastructure MeSH
- Molecular Conformation MeSH
- Models, Molecular * MeSH
- Molecular Structure MeSH
- Surface Properties MeSH
- Antineoplastic Agents administration & dosage chemistry metabolism MeSH
- RNA Interference MeSH
- Ruthenium administration & dosage chemistry metabolism MeSH
- Silanes chemistry metabolism MeSH
- Molecular Dynamics Simulation MeSH
- Drug Stability MeSH
- RNA Stability MeSH
- Microscopy, Electron, Transmission MeSH
- Particle Size MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
Dendrimers, which are considered as one of the most promising tools in the field of nanobiotechnology due to their structural organization, showed a great potential in gene therapy, drug delivery, medical imaging and as antimicrobial and antiviral agents. This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into the cancer cells. Moreover, dendrimers can protect siRNA against nuclease degradation. Nevertheless, further research need to be performed to examine the therapeutic potential of ruthenium dendrimers as well as dendrimers complexed with siRNA and anticancer drugs towards cancer cells.
Departamento Química Orgánica y Química Inorganica Universidad de Alcala de Henares Spain
Department of General Biophysics University of Lodz Pomorska 141 143 90 236 Lodz Poland
Institute of Biophysics and Cell Engineering of NASB Akademicheskaja 27 Minsk 220072 Belarus
Networking Research Center on Bioengineering Biomaterials and Nanomedicine Spain
References provided by Crossref.org
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- $a Michlewska, Sylwia $u Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz, Banacha12/16, 90-237 Lodz, Poland; Department of General Biophysics, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland.
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- $a Dendrimers, which are considered as one of the most promising tools in the field of nanobiotechnology due to their structural organization, showed a great potential in gene therapy, drug delivery, medical imaging and as antimicrobial and antiviral agents. This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into the cancer cells. Moreover, dendrimers can protect siRNA against nuclease degradation. Nevertheless, further research need to be performed to examine the therapeutic potential of ruthenium dendrimers as well as dendrimers complexed with siRNA and anticancer drugs towards cancer cells.
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