The carbosilane metallodendrimer G1-[[NCPh(o-N)Ru(η6- p-cymene)Cl]Cl]4 (CRD13), based on an arene Ru(II) complex coordinated to imino-pyridine surface groups, has been conjugated with anti-cancer drugs. Ruthenium in the positively-charged dendrimer structure allows this nanoparticle to be considered as an anticancer drug carrier, made more efficient because ruthenium has anticancer properties. The ability of CRD13 to form complexes with Doxorubicin (DOX), 5-Fluorouracil (5-Fu), and Methotrexate (MTX) has been evaluated using zeta potential measurement, transmission electron microscopy (TEM) and computer simulation. The results show that it forms stable nanocomplexes with all those drugs, enhancing their effectiveness against MDA-MB-231 cancer cells. In vivo tests indicate that the CRD13/DOX system caused a decrease of tumor weight in mice with triple negative breast cancer. However, the tumors were most visibly reduced when naked dendrimers were injected.
- MeSH
- komplexní sloučeniny * chemie MeSH
- lidé MeSH
- molekulární struktura MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- nosiče léků MeSH
- počítačová simulace MeSH
- protinádorové látky * chemie MeSH
- ruthenium * chemie MeSH
- screeningové testy protinádorových léčiv MeSH
- triple-negativní karcinom prsu * farmakoterapie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Dendrimers, which are considered as one of the most promising tools in the field of nanobiotechnology due to their structural organization, showed a great potential in gene therapy, drug delivery, medical imaging and as antimicrobial and antiviral agents. This article is devoted to study interactions between new carbosilane-based metallodendrimers containing ruthenium and anti-cancer small interfering RNA (siRNA). Formation of complexes between anti-cancer siRNAs and Ru-based carbosilane dendrimers was evaluated by transmission electron microscopy, circular dichroism and fluorescence. The zeta-potential and the size of dendriplexes were determined by dynamic light scattering. The internalization of dendriplexes were estimated using HL-60 cells. Results show that ruthenium dendrimers associated with anticancer siRNA have the ability to deliver siRNA as non-viral vectors into the cancer cells. Moreover, dendrimers can protect siRNA against nuclease degradation. Nevertheless, further research need to be performed to examine the therapeutic potential of ruthenium dendrimers as well as dendrimers complexed with siRNA and anticancer drugs towards cancer cells.
- MeSH
- akutní promyelocytární leukemie farmakoterapie metabolismus patologie MeSH
- cirkulární dichroismus MeSH
- dendrimery aplikace a dávkování chemie metabolismus MeSH
- dynamický rozptyl světla MeSH
- fyziologická absorpce MeSH
- HL-60 buňky MeSH
- interkalátory aplikace a dávkování chemie metabolismus MeSH
- lidé MeSH
- malá interferující RNA aplikace a dávkování chemie metabolismus ultrastruktura MeSH
- molekulární konformace MeSH
- molekulární modely * MeSH
- molekulární struktura MeSH
- povrchové vlastnosti MeSH
- protinádorové látky aplikace a dávkování chemie metabolismus MeSH
- RNA interference MeSH
- ruthenium aplikace a dávkování chemie metabolismus MeSH
- silany chemie metabolismus MeSH
- simulace molekulární dynamiky MeSH
- stabilita léku MeSH
- stabilita RNA MeSH
- transmisní elektronová mikroskopie MeSH
- velikost částic MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
