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A human pericardium biopolymeric scaffold for autologous heart valve tissue engineering: cellular and extracellular matrix structure and biomechanical properties in comparison with a normal aortic heart valve

F. Straka, D. Schornik, J. Masin, E. Filova, T. Mirejovsky, Z. Burdikova, Z. Svindrych, H. Chlup, L. Horny, M. Daniel, J. Machac, J. Skibová, J. Pirk, L. Bacakova,

. 2018 ; 29 (6) : 599-634. [pub] 20180130

Language English Country England, Great Britain

Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19000981

Grant support
NV15-27941A MZ0 CEP Register
NV15-29153A MZ0 CEP Register

The objective of our study was to compare the cellular and extracellular matrix (ECM) structure and the biomechanical properties of human pericardium (HP) with the normal human aortic heart valve (NAV). HP tissues (from 12 patients) and NAV samples (from 5 patients) were harvested during heart surgery. The main cells in HP were pericardial interstitial cells, which are fibroblast-like cells of mesenchymal origin similar to the valvular interstitial cells in NAV tissue. The ECM of HP had a statistically significantly (p < 0.001) higher collagen I content, a lower collagen III and elastin content, and a similar glycosaminoglycans (GAGs) content, in comparison with the NAV, as measured by ECM integrated density. However, the relative thickness of the main load-bearing structures of the two tissues, the dense part of fibrous HP (49 ± 2%) and the lamina fibrosa of NAV (47 ± 4%), was similar. In both tissues, the secant elastic modulus (Es) was significantly lower in the transversal direction (p < 0.05) than in the longitudinal direction. This proved that both tissues were anisotropic. No statistically significant differences in UTS (ultimate tensile strength) values and in calculated bending stiffness values in the longitudinal or transversal direction were found between HP and NAV. Our study confirms that HP has an advantageous ECM biopolymeric structure and has the biomechanical properties required for a tissue from which an autologous heart valve replacement may be constructed.

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$a The objective of our study was to compare the cellular and extracellular matrix (ECM) structure and the biomechanical properties of human pericardium (HP) with the normal human aortic heart valve (NAV). HP tissues (from 12 patients) and NAV samples (from 5 patients) were harvested during heart surgery. The main cells in HP were pericardial interstitial cells, which are fibroblast-like cells of mesenchymal origin similar to the valvular interstitial cells in NAV tissue. The ECM of HP had a statistically significantly (p < 0.001) higher collagen I content, a lower collagen III and elastin content, and a similar glycosaminoglycans (GAGs) content, in comparison with the NAV, as measured by ECM integrated density. However, the relative thickness of the main load-bearing structures of the two tissues, the dense part of fibrous HP (49 ± 2%) and the lamina fibrosa of NAV (47 ± 4%), was similar. In both tissues, the secant elastic modulus (Es) was significantly lower in the transversal direction (p < 0.05) than in the longitudinal direction. This proved that both tissues were anisotropic. No statistically significant differences in UTS (ultimate tensile strength) values and in calculated bending stiffness values in the longitudinal or transversal direction were found between HP and NAV. Our study confirms that HP has an advantageous ECM biopolymeric structure and has the biomechanical properties required for a tissue from which an autologous heart valve replacement may be constructed.
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$a Schornik, David $u Department of Biomaterials and Tissue Engineering , Institute of Physiology, Academy of Sciences of the Czech Republic , Prague , Czech Republic.
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$a Masin, Jaroslav $u Cardiology Centre and Cardiovascular Surgery Department , Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
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$a Filova, Elena $u Department of Biomaterials and Tissue Engineering , Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
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$a Mirejovsky, Tomas $u Clinical and Transplant Pathology Department, Institute for Clinical and Experimental Medicine , Prague , Czech Republic.
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$a Svindrych, Zdenek $u Department of Biology, W. M, Keck Center for Cellular Imaging , University of Virginia, Charlottesville, VA, USA.
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$a Chlup, Hynek $u Faculty of Mechanical Engineering, Department of Mechanics, Biomechanics and Mechatronics, Czech Technical University in Prague, Prague, Czech Republic.
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$a Horny, Lukas $u Faculty of Mechanical Engineering, Department of Mechanics, Biomechanics and Mechatronics, Czech Technical University in Prague, Prague, Czech Republic.
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$a Daniel, Matej, $d 1978- $7 xx0029605 $u Faculty of Mechanical Engineering, Department of Mechanics, Biomechanics and Mechatronics, Czech Technical University in Prague, Prague, Czech Republic.
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$a Machac, Jiri $u Institute of Botany CAS, Academy of Sciences of the Czech Republic, Pruhonice, Czech Republic.
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$a Skibová, Jelena $u Department of Medical Statistics, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
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$a Pirk, Jan $u Cardiology Centre and Cardiovascular Surgery Department, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.
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$a Bacakova, Lucie $u Department of Biomaterials and Tissue Engineering, Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
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