• Je něco špatně v tomto záznamu ?

Binding of pigments to the cyanobacterial high-light-inducible protein HliC

MK. Shukla, MJ. Llansola-Portoles, M. Tichý, AA. Pascal, B. Robert, R. Sobotka,

. 2018 ; 137 (1) : 29-39. [pub] 20171226

Jazyk angličtina Země Nizozemsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19001011
E-zdroje Online Plný text

NLK ProQuest Central od 1997-01-01 do Před 1 rokem
Medline Complete (EBSCOhost) od 2011-01-01 do Před 1 rokem
Health & Medicine (ProQuest) od 1997-01-01 do Před 1 rokem

Odkazy

PubMed 29280045
DOI 10.1007/s11120-017-0475-7
Knihovny.cz E-zdroje

Cyanobacteria possess a family of one-helix high-light-inducible proteins (HLIPs) that are widely viewed as ancestors of the light-harvesting antenna of plants and algae. HLIPs are essential for viability under various stress conditions, although their exact role is not fully understood. The unicellular cyanobacterium Synechocystis sp. PCC 6803 contains four HLIPs named HliA-D, and HliD has recently been isolated in a small protein complex and shown to bind chlorophyll and β-carotene. However, no HLIP has been isolated and characterized in a pure form up to now. We have developed a protocol to purify large quantities of His-tagged HliC from an engineered Synechocystis strain. Purified His-HliC is a pigmented homo-oligomer and is associated with chlorophyll and β-carotene with a 2:1 ratio. This differs from the 3:1 ratio reported for HliD. Comparison of these two HLIPs by resonance Raman spectroscopy revealed a similar conformation for their bound β-carotenes, but clear differences in their chlorophylls. We present and discuss a structural model of HliC, in which a dimeric protein binds four chlorophyll molecules and two β-carotenes.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19001011
003      
CZ-PrNML
005      
20190122110305.0
007      
ta
008      
190107s2018 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s11120-017-0475-7 $2 doi
035    __
$a (PubMed)29280045
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Shukla, Mahendra Kumar $u Centre Algatech, Institute of Microbiology, Academy of Sciences of the Czech Republic, 379 81, Třeboň, Czech Republic. Faculty of Science, University of South Bohemia, 370 01, České Budějovice, Czech Republic.
245    10
$a Binding of pigments to the cyanobacterial high-light-inducible protein HliC / $c MK. Shukla, MJ. Llansola-Portoles, M. Tichý, AA. Pascal, B. Robert, R. Sobotka,
520    9_
$a Cyanobacteria possess a family of one-helix high-light-inducible proteins (HLIPs) that are widely viewed as ancestors of the light-harvesting antenna of plants and algae. HLIPs are essential for viability under various stress conditions, although their exact role is not fully understood. The unicellular cyanobacterium Synechocystis sp. PCC 6803 contains four HLIPs named HliA-D, and HliD has recently been isolated in a small protein complex and shown to bind chlorophyll and β-carotene. However, no HLIP has been isolated and characterized in a pure form up to now. We have developed a protocol to purify large quantities of His-tagged HliC from an engineered Synechocystis strain. Purified His-HliC is a pigmented homo-oligomer and is associated with chlorophyll and β-carotene with a 2:1 ratio. This differs from the 3:1 ratio reported for HliD. Comparison of these two HLIPs by resonance Raman spectroscopy revealed a similar conformation for their bound β-carotenes, but clear differences in their chlorophylls. We present and discuss a structural model of HliC, in which a dimeric protein binds four chlorophyll molecules and two β-carotenes.
650    _2
$a bakteriální proteiny $x chemie $x genetika $x izolace a purifikace $x metabolismus $7 D001426
650    _2
$a chlorofyl $x metabolismus $7 D002734
650    _2
$a světlosběrné proteinové komplexy $x genetika $x metabolismus $7 D045342
650    _2
$a multimerizace proteinu $7 D055503
650    _2
$a rekombinantní proteiny $x genetika $x izolace a purifikace $x metabolismus $7 D011994
650    _2
$a Ramanova spektroskopie $7 D013059
650    _2
$a Synechocystis $x genetika $x metabolismus $x fyziologie $7 D046939
650    _2
$a beta-karoten $x metabolismus $7 D019207
655    _2
$a časopisecké články $7 D016428
700    1_
$a Llansola-Portoles, Manuel J $u Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
700    1_
$a Tichý, Martin $u Centre Algatech, Institute of Microbiology, Academy of Sciences of the Czech Republic, 379 81, Třeboň, Czech Republic.
700    1_
$a Pascal, Andrew A $u Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
700    1_
$a Robert, Bruno $u Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198, Gif-sur-Yvette cedex, France.
700    1_
$a Sobotka, Roman $u Centre Algatech, Institute of Microbiology, Academy of Sciences of the Czech Republic, 379 81, Třeboň, Czech Republic. sobotka@alga.cz. Faculty of Science, University of South Bohemia, 370 01, České Budějovice, Czech Republic. sobotka@alga.cz.
773    0_
$w MED00006488 $t Photosynthesis research $x 1573-5079 $g Roč. 137, č. 1 (2018), s. 29-39
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29280045 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190107 $b ABA008
991    __
$a 20190122110524 $b ABA008
999    __
$a ok $b bmc $g 1364962 $s 1039134
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 137 $c 1 $d 29-39 $e 20171226 $i 1573-5079 $m Photosynthesis research $n Photosynth Res $x MED00006488
LZP    __
$a Pubmed-20190107

Najít záznam

Citační ukazatele

Možnosti archivace