• Je něco špatně v tomto záznamu ?

Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia

M. Moder, G. Velimezi, M. Owusu, A. Mazouzi, M. Wiedner, J. Ferreira da Silva, L. Robinson-Garcia, F. Schischlik, R. Slavkovsky, R. Kralovics, M. Schuster, C. Bock, T. Ideker, SP. Jackson, J. Menche, JI. Loizou,

. 2017 ; 8 (1) : 1238. [pub] 20171101

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc19001120

Maintenance of genome integrity via repair of DNA damage is a key biological process required to suppress diseases, including Fanconi anemia (FA). We generated loss-of-function human haploid cells for FA complementation group C (FANCC), a gene encoding a component of the FA core complex, and used genome-wide CRISPR libraries as well as insertional mutagenesis to identify synthetic viable (genetic suppressor) interactions for FA. Here we show that loss of the BLM helicase complex suppresses FANCC phenotypes and we confirm this interaction in cells deficient for FA complementation group I and D2 (FANCI and FANCD2) that function as part of the FA I-D2 complex, indicating that this interaction is not limited to the FA core complex, hence demonstrating that systematic genome-wide screening approaches can be used to reveal genetic viable interactions for DNA repair defects.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19001120
003      
CZ-PrNML
005      
20190108130209.0
007      
ta
008      
190107s2017 enk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41467-017-01439-x $2 doi
035    __
$a (PubMed)29089570
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a enk
100    1_
$a Moder, Martin $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
245    10
$a Parallel genome-wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia / $c M. Moder, G. Velimezi, M. Owusu, A. Mazouzi, M. Wiedner, J. Ferreira da Silva, L. Robinson-Garcia, F. Schischlik, R. Slavkovsky, R. Kralovics, M. Schuster, C. Bock, T. Ideker, SP. Jackson, J. Menche, JI. Loizou,
520    9_
$a Maintenance of genome integrity via repair of DNA damage is a key biological process required to suppress diseases, including Fanconi anemia (FA). We generated loss-of-function human haploid cells for FA complementation group C (FANCC), a gene encoding a component of the FA core complex, and used genome-wide CRISPR libraries as well as insertional mutagenesis to identify synthetic viable (genetic suppressor) interactions for FA. Here we show that loss of the BLM helicase complex suppresses FANCC phenotypes and we confirm this interaction in cells deficient for FA complementation group I and D2 (FANCI and FANCD2) that function as part of the FA I-D2 complex, indicating that this interaction is not limited to the FA core complex, hence demonstrating that systematic genome-wide screening approaches can be used to reveal genetic viable interactions for DNA repair defects.
650    _2
$a CRISPR-Cas systémy $7 D064113
650    _2
$a buněčné linie $7 D002460
650    _2
$a poškození DNA $7 D004249
650    _2
$a DNA-helikasy $x genetika $7 D004265
650    _2
$a oprava DNA $x genetika $7 D004260
650    _2
$a Fanconiho anemie $x genetika $7 D005199
650    _2
$a protein FANCC $x genetika $7 D052218
650    _2
$a protein FANCD2 $x genetika $7 D052236
650    _2
$a proteiny FANC $x genetika $7 D051856
650    _2
$a HEK293 buňky $7 D057809
650    _2
$a haploidie $7 D006238
650    _2
$a lidé $7 D006801
650    _2
$a inzerční mutageneze $7 D016254
650    _2
$a NAD(P)H dehydrogenasa (chinon) $x genetika $7 D016660
650    _2
$a helikasy RecQ $x genetika $7 D053484
655    _2
$a časopisecké články $7 D016428
655    _2
$a Research Support, N.I.H., Extramural $7 D052061
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Velimezi, Georgia $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
700    1_
$a Owusu, Michel $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
700    1_
$a Mazouzi, Abdelghani $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
700    1_
$a Wiedner, Marc $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
700    1_
$a Ferreira da Silva, Joana $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
700    1_
$a Robinson-Garcia, Lydia $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
700    1_
$a Schischlik, Fiorella $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
700    1_
$a Slavkovsky, Rastislav $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria. Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic.
700    1_
$a Kralovics, Robert $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
700    1_
$a Schuster, Michael $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
700    1_
$a Bock, Christoph $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
700    1_
$a Ideker, Trey $u Department of Medicine, Division of Genetics, University of California San Diego, La Jolla, CA, 92093, USA. Department of Bioengineering, University of California San Diego, La Jolla, CA, 92093, USA. Moores Cancer Center, University of California San Diego, La Jolla, CA, 92093, USA. The Cancer Cell Map Initiative, La Jolla, CA, 92093, USA.
700    1_
$a Jackson, Stephen P $u The Wellcome Trust and Cancer Research UK Gurdon Institute, and Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QN, UK. The Wellcome Trust Sanger Institute, Hinxton, Cambridge, CB10 1SA, UK.
700    1_
$a Menche, Jörg $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria.
700    1_
$a Loizou, Joanna I $u CeMM Research Center for Molecular Medicine of the Austrian Academy of Sciences, Lazarettgasse 14, AKH BT 25.3, 1090, Vienna, Austria. jloizou@cemm.oeaw.ac.at.
773    0_
$w MED00184850 $t Nature communications $x 2041-1723 $g Roč. 8, č. 1 (2017), s. 1238
856    41
$u https://pubmed.ncbi.nlm.nih.gov/29089570 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190107 $b ABA008
991    __
$a 20190108130410 $b ABA008
999    __
$a ok $b bmc $g 1365039 $s 1039243
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2017 $b 8 $c 1 $d 1238 $e 20171101 $i 2041-1723 $m Nature communications $n Nat Commun $x MED00184850
LZP    __
$a Pubmed-20190107

Najít záznam

Citační ukazatele

Možnosti archivace