-
Something wrong with this record ?
Calcaneal Tendon Collagen Fiber Morphometry and Aging
D. Hadraba, J. Janacek, E. Filova, F. Lopot, R. Paesen, O. Fanta, A. Jarman, A. Necas, M. Ameloot, K. Jelen,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NV16-28637A
MZ0
CEP Register
Digital library NLK
Full text - Article
NLK
ProQuest Central
from 2002-02-01 to 2022-12-31
Nursing & Allied Health Database (ProQuest)
from 2002-02-01 to 2022-12-31
Health & Medicine (ProQuest)
from 2002-02-01 to 2022-12-31
- MeSH
- Achilles Tendon cytology growth & development ultrastructure MeSH
- Biomechanical Phenomena physiology MeSH
- Extracellular Matrix physiology MeSH
- Fibrillar Collagens metabolism ultrastructure MeSH
- Microscopy, Confocal MeSH
- Rabbits MeSH
- Microscopy, Electron, Scanning MeSH
- Tensile Strength physiology MeSH
- Microscopy, Polarization MeSH
- Aging physiology MeSH
- Animals MeSH
- Check Tag
- Rabbits MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Fibrillar collagen in tendons and its natural development in rabbits are discussed in this paper. Achilles tendons from newborn (~7 days) to elderly (~38 months) rabbits were monitored in intact (n tendons=24) and microtome sectioned (n tendons=11) states with label-free second harmonic generation microscopy. After sectioning, the collagen fiber pattern was irregular for the younger animals and remained oriented parallel to the load axis of the tendon for the older animals. In contrast, the collagen fiber pattern in the intact samples followed the load axis for all the age groups. However, there was a significant difference in the tendon crimp pattern appearance between the age groups. The crimp amplitude (A) and wavelength (Λ) started at very low values (A=2.0±0.6 µm, Λ=19±4 µm) for the newborn animals. Both parameters increased for the sexually mature animals (>5 months old). When the animals were fully mature the amplitude decreased but the wavelength kept increasing. The results revealed that the microtome sectioning artifacts depend on the age of animals and that the collagen crimp pattern reflects the physical growth and development.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc19001181
- 003
- CZ-PrNML
- 005
- 20190122121901.0
- 007
- ta
- 008
- 190107s2017 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1017/S1431927617012569 $2 doi
- 035 __
- $a (PubMed)28929998
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Hadraba, Daniel $u 1Department of Biomathematics,Institute of Physiology,The Czech Academy of Sciences,Videnska 1083,Prague 4,14220,Czech Republic.
- 245 10
- $a Calcaneal Tendon Collagen Fiber Morphometry and Aging / $c D. Hadraba, J. Janacek, E. Filova, F. Lopot, R. Paesen, O. Fanta, A. Jarman, A. Necas, M. Ameloot, K. Jelen,
- 520 9_
- $a Fibrillar collagen in tendons and its natural development in rabbits are discussed in this paper. Achilles tendons from newborn (~7 days) to elderly (~38 months) rabbits were monitored in intact (n tendons=24) and microtome sectioned (n tendons=11) states with label-free second harmonic generation microscopy. After sectioning, the collagen fiber pattern was irregular for the younger animals and remained oriented parallel to the load axis of the tendon for the older animals. In contrast, the collagen fiber pattern in the intact samples followed the load axis for all the age groups. However, there was a significant difference in the tendon crimp pattern appearance between the age groups. The crimp amplitude (A) and wavelength (Λ) started at very low values (A=2.0±0.6 µm, Λ=19±4 µm) for the newborn animals. Both parameters increased for the sexually mature animals (>5 months old). When the animals were fully mature the amplitude decreased but the wavelength kept increasing. The results revealed that the microtome sectioning artifacts depend on the age of animals and that the collagen crimp pattern reflects the physical growth and development.
- 650 _2
- $a Achillova šlacha $x cytologie $x růst a vývoj $x ultrastruktura $7 D000125
- 650 _2
- $a stárnutí $x fyziologie $7 D000375
- 650 _2
- $a zvířata $7 D000818
- 650 _2
- $a biomechanika $x fyziologie $7 D001696
- 650 _2
- $a extracelulární matrix $x fyziologie $7 D005109
- 650 _2
- $a fibrilární kolageny $x metabolismus $x ultrastruktura $7 D024022
- 650 _2
- $a konfokální mikroskopie $7 D018613
- 650 _2
- $a mikroskopie elektronová rastrovací $7 D008855
- 650 _2
- $a polarizační mikroskopie $7 D008859
- 650 _2
- $a králíci $7 D011817
- 650 _2
- $a pevnost v tahu $x fyziologie $7 D013718
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Janacek, Jiri $u 1Department of Biomathematics,Institute of Physiology,The Czech Academy of Sciences,Videnska 1083,Prague 4,14220,Czech Republic.
- 700 1_
- $a Filova, Eva $u 4Department of Tissue Engineering,Institute of Experimental Medicine,The Czech Academy of Sciences,Videnska 1083,Prague 4,14220,Czech Republic.
- 700 1_
- $a Lopot, Frantisek $u 2Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport,Charles University,Jose Martiho 31,Prague 6,162 00,Czech Republic.
- 700 1_
- $a Paesen, Rik $u 3Department of Biophysics, Biomedical Research Institute,Hasselt University,Agoralaan building C,Diepenbeek,B-3590,Belgium.
- 700 1_
- $a Fanta, Ondrej $u 2Department of Anatomy and Biomechanics, Faculty of Physical Education and Sport,Charles University,Jose Martiho 31,Prague 6,162 00,Czech Republic.
- 700 1_
- $a Jarman, Anneliese $u 5Department of Tissue Engineering & Biophotonics,King's College London,Guy's Campus,Great Maze Pond,London,SE1 9RT,UK.
- 700 1_
- $a Necas, Alois $u 6Faculty of Veterinary Medicine,University of Veterinary and Pharmaceutical Sciences Brno,Palackeho tr. 1/3,Brno,612 42,Czech Republic.
- 700 1_
- $a Ameloot, Marcel $u 3Department of Biophysics, Biomedical Research Institute,Hasselt University,Agoralaan building C,Diepenbeek,B-3590,Belgium.
- 700 1_
- $a Jelen, Karel $u 1Department of Biomathematics,Institute of Physiology,The Czech Academy of Sciences,Videnska 1083,Prague 4,14220,Czech Republic.
- 773 0_
- $w MED00005775 $t Microscopy and microanalysis the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada $x 1435-8115 $g Roč. 23, č. 5 (2017), s. 1040-1047
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/28929998 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20190107 $b ABA008
- 991 __
- $a 20190122122120 $b ABA008
- 999 __
- $a ok $b bmc $g 1364022 $s 1039304
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2017 $b 23 $c 5 $d 1040-1047 $e 20170920 $i 1435-8115 $m Microscopy and microanalysis $n Microsc Microanal $x MED00005775
- GRA __
- $a NV16-28637A $p MZ0
- LZP __
- $a Pubmed-20190107
