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ZNF143 protein is an important regulator of the myeloid transcription factor C/EBPα
D. Gonzalez, A. Luyten, B. Bartholdy, Q. Zhou, M. Kardosova, A. Ebralidze, KD. Swanson, HS. Radomska, P. Zhang, SS. Kobayashi, RS. Welner, E. Levantini, U. Steidl, G. Chong, S. Collombet, MH. Choi, AD. Friedman, LM. Scott, M. Alberich-Jorda, DG. Tenen,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, Research Support, N.I.H., Extramural, práce podpořená grantem
NLK
Free Medical Journals
od 2008 do Před 1 rokem
Freely Accessible Science Journals
od 1905 do Před 1 rokem
PubMed Central
od 2005
Europe PubMed Central
od 2005 do Před 1 rokem
Open Access Digital Library
od 1905-10-01
Open Access Digital Library
od 1905-10-01
ROAD: Directory of Open Access Scholarly Resources
od 1905
PubMed
28900037
DOI
10.1074/jbc.m117.811109
Knihovny.cz E-zdroje
- MeSH
- aktivace transkripce * MeSH
- buněčné linie MeSH
- hematopoéza MeSH
- konzervovaná sekvence MeSH
- lidé MeSH
- myeloidní buňky cytologie metabolismus MeSH
- promotorové oblasti (genetika) * MeSH
- protein alfa vázající zesilovač transkripce CCAAT genetika MeSH
- sekvence nukleotidů MeSH
- transaktivátory metabolismus MeSH
- vazba proteinů MeSH
- vývojová regulace genové exprese MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
The transcription factor C/EBPα is essential for myeloid differentiation and is frequently dysregulated in acute myeloid leukemia. Although studied extensively, the precise regulation of its gene by upstream factors has remained largely elusive. Here, we investigated its transcriptional activation during myeloid differentiation. We identified an evolutionarily conserved octameric sequence, CCCAGCAG, ∼100 bases upstream of the CEBPA transcription start site, and demonstrated through mutational analysis that this sequence is crucial for C/EBPα expression. This sequence is present in the genes encoding C/EBPα in humans, rodents, chickens, and frogs and is also present in the promoters of other C/EBP family members. We identified that ZNF143, the human homolog of the Xenopus transcriptional activator STAF, specifically binds to this 8-bp sequence to activate C/EBPα expression in myeloid cells through a mechanism that is distinct from that observed in liver cells and adipocytes. Altogether, our data suggest that ZNF143 plays an important role in the expression of C/EBPα in myeloid cells.
From the Cancer Science Institute National University of Singapore 117599 Singapore
the Beth Israel Deaconess Medical Center Harvard Medical School Boston Massachusetts 02115
the Harvard Stem Cell Institute Harvard Medical School Boston Massachusetts 02115
Citace poskytuje Crossref.org
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- $a The transcription factor C/EBPα is essential for myeloid differentiation and is frequently dysregulated in acute myeloid leukemia. Although studied extensively, the precise regulation of its gene by upstream factors has remained largely elusive. Here, we investigated its transcriptional activation during myeloid differentiation. We identified an evolutionarily conserved octameric sequence, CCCAGCAG, ∼100 bases upstream of the CEBPA transcription start site, and demonstrated through mutational analysis that this sequence is crucial for C/EBPα expression. This sequence is present in the genes encoding C/EBPα in humans, rodents, chickens, and frogs and is also present in the promoters of other C/EBP family members. We identified that ZNF143, the human homolog of the Xenopus transcriptional activator STAF, specifically binds to this 8-bp sequence to activate C/EBPα expression in myeloid cells through a mechanism that is distinct from that observed in liver cells and adipocytes. Altogether, our data suggest that ZNF143 plays an important role in the expression of C/EBPα in myeloid cells.
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