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Processing of mRNA from human leukocytes by biomagnetical separation: comparison with current methods of RNA isolation
F. Mrázek, M. Petřek
Acta Universitatis Palackianae Olomucensis Facultatis medicae. Biomedical papers.
1999 ;
142 (142) :
23-28.
Jazyk angličtina Země Česko
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem, přehledy
Perzistentní odkaz
https://www.medvik.cz/link/bmc19007718
- MeSH
- bronchoalveolární lavážní tekutina cytologie MeSH
- leukocyty chemie MeSH
- lidé MeSH
- magnetismus MeSH
- messenger RNA krev izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- srovnávací studie MeSH
The article refers to the technique of biomagnetical separation (BS) of mRNA using "Dynabeads mRNA DIRECT". After a brief survey of RNA isolation methods authors describe a general procedure of BS as well as its concrete application for mRNA isolation from bronchoalveolar cells. Authors report results of specific experiments to assess the efficiency and accuracy of mRNA isolation by BS. The applicability of BS technique for the gene expression studies is discussed in the end of the article.
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