Trichosporon cutaneum is the second most significant opportunistic pathogen of genus Trichosporon after T. asahii . Its virulence is strongly tied to biofilm formation and colonization of various medical materials. Tricho- sporon spp. infections are ordinarily treated with antifun- gal azoles or polyene antibiotics. Nowadays, this type of therapy often fails and there is a great effort aimed at find- ing new antimicrobial substances, often originating from plant sources. These sources are frequently used as the components of traditional medicine. This study is focused on the effect of antibiotic am- photericin B, polysaccharide chitosan, and flavonoid bai- calein on the biofilm formation of T. cutaneum CCY 30-5-10. Their effects were studied by inverse microscope Cel- lavista using the image analysis evaluation of the area colonized by the biofilm in 96well microtitre plates. Baicalein was the most effective substance (concentration 40 mg L ‒ 1 caused 50% inhibition of cell adhesion and 75% eradication of mature biofilm). A significant effect was also observed when chitosan was applied (concentration 40 mg L ‒ 1 caused 69% inhibition of cell adhesion). Polyene antibiotic amphotericin B had distinctly stimulating effect on cell adhesion and only slightly eradicated the mature biofilm of T. cutaneum CCY 30-5-10.

Ústav biotechnologie Vysoká škola chemicko technologická Praha Praha

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