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Paeonia arietina and Paeonia kesrounansis bioactive constituents: NMR, LC-DAD-MS fingerprinting and in vitro assays

S. Sut, G. Zengin, S. Dall'Acqua, M. Gazdová, K. Šmejkal, G. Bulut, A. Dogan, MZ. Haznedaroglu, MZ. Aumeeruddy, F. Maggi, MF. Mahomoodally,

. 2019 ; 165 (-) : 1-11. [pub] 20181119

Jazyk angličtina Země Anglie, Velká Británie

Typ dokumentu srovnávací studie, časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc19012105

Paeonia species have been valued for their ethnomedicinal uses in various countries and received much interest among the scientific community for their therapeutic properties, including anti-microbial, anti-inflammatory, anti-cancer, nephroprotective and hepatoprotective effects. The multiple phytotherapeutical applications of Paeonia species inspired us to establish the phytochemical fingerprint and to evaluate the biological properties of ethyl acetate, methanol, and aqueous extracts from the roots and aerial parts of two Paeonia species (P. arietina G. Anderson and P. kesrounansis Thiébaut). Phytoconstituents of P. arietina and P. kesrounansis extracts were analyzed using 1D and 2D NMR and LC-DAD-ESI-MS. The total content of phenolics (TPC) and flavonoids (TFC) in the extracts was also evaluated. The antioxidant activity was profiled using DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation assays. Enzyme inhibitory properties were evaluated against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase. Phytochemical analysis of P. arietina and P. kesrounansis extracts showed the presence of galloyl esters of sugars, galloyl monoterpenes, and glycosylated flavonoids. The three solvent extracts presented different behavior in the bioassays. The highest antioxidant activity, tyrosinase and AChE inhibition were observed for the methanolic extract of the aerial parts of P. kesrounansis. In addition, the ethyl acetate extracts of the aerial parts of both plants were the most effective inhibitors of α-amylase. The highest BChE inhibition was observed for root methanolic extract of P. kesrounansis while the root ethyl acetate extract of P. arietina exerted the strongest inhibition of α-glucosidase. Methanol extract of P. kesrounansis aerial parts presented the highest TPC, while TFC was greatest in the corresponding extract of P. arietina. Our findings can be considered as a starting point for future studies to further validate the effectiveness and safety profiles of these plants in folk medicine.

Citace poskytuje Crossref.org

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$a Sut, Stefania $u DAFNAE, Department of Agronomy, Food, Natural resources, Animals and Environment, University of Padova, Viale dell'Università, 16, 35020 Legnaro, PD, Italy. Electronic address: stefania_sut@hotmail.it.
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$a Paeonia species have been valued for their ethnomedicinal uses in various countries and received much interest among the scientific community for their therapeutic properties, including anti-microbial, anti-inflammatory, anti-cancer, nephroprotective and hepatoprotective effects. The multiple phytotherapeutical applications of Paeonia species inspired us to establish the phytochemical fingerprint and to evaluate the biological properties of ethyl acetate, methanol, and aqueous extracts from the roots and aerial parts of two Paeonia species (P. arietina G. Anderson and P. kesrounansis Thiébaut). Phytoconstituents of P. arietina and P. kesrounansis extracts were analyzed using 1D and 2D NMR and LC-DAD-ESI-MS. The total content of phenolics (TPC) and flavonoids (TFC) in the extracts was also evaluated. The antioxidant activity was profiled using DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, and metal chelation assays. Enzyme inhibitory properties were evaluated against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, α-amylase, and α-glucosidase. Phytochemical analysis of P. arietina and P. kesrounansis extracts showed the presence of galloyl esters of sugars, galloyl monoterpenes, and glycosylated flavonoids. The three solvent extracts presented different behavior in the bioassays. The highest antioxidant activity, tyrosinase and AChE inhibition were observed for the methanolic extract of the aerial parts of P. kesrounansis. In addition, the ethyl acetate extracts of the aerial parts of both plants were the most effective inhibitors of α-amylase. The highest BChE inhibition was observed for root methanolic extract of P. kesrounansis while the root ethyl acetate extract of P. arietina exerted the strongest inhibition of α-glucosidase. Methanol extract of P. kesrounansis aerial parts presented the highest TPC, while TFC was greatest in the corresponding extract of P. arietina. Our findings can be considered as a starting point for future studies to further validate the effectiveness and safety profiles of these plants in folk medicine.
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$a Zengin, Gokhan $u Selcuk University, Science Faculty, Department of Biology, Campus, 42250, Konya, Turkey. Electronic address: gokhanzengin@selcuk.edu.tr.
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$a Dall'Acqua, Stefano $u NPL lab, Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo, 35121, Padova, Italy. Electronic address: stefano.dallacqua@unipd.it.
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$a Gazdová, Markéta $u Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1/3, 612 42, Brno, Czech Republic. Electronic address: gazdova.marketa@seznam.cz. $7 xx0270941
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$a Šmejkal, Karel $u Department of Natural Drugs, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1/3, 612 42, Brno, Czech Republic. Electronic address: karel.mejkal@post.cz.
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$a Bulut, Gizem $u Marmara University, Pharmacy Faculty, Department of Pharmaceutical Botany, Istanbul, Turkey. Electronic address: gizem.bulut@marmara.edu.tr.
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$a Dogan, Ahmet $u Marmara University, Pharmacy Faculty, Department of Pharmaceutical Botany, Istanbul, Turkey. Electronic address: adogan@marmara.edu.tr.
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$a Haznedaroglu, Mehmet Zeki $u Izmir Katip Celebi University, Pharmacy Faculty, Department of Pharmaceutical Botany, Izmir, Turkey. Electronic address: zeki.haznedaroglu@ikc.edu.tr.
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$a Aumeeruddy, Muhammad Zakariyyah $u Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, Mauritius. Electronic address: muhammad.aumeeruddy2@umail.uom.ac.mu.
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$a Maggi, Filippo $u School of Pharmacy, University of Camerino, Camerino, Italy. Electronic address: filippo.maggi@unicam.it.
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$a Mahomoodally, Mohamad Fawzi $u Department of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, Mauritius. Electronic address: f.mahomoodally@uom.ac.mu.
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