Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Genetic Resistance to Avian Leukosis Viruses Induced by CRISPR/Cas9 Editing of Specific Receptor Genes in Chicken Cells

A. Koslová, D. Kučerová, M. Reinišová, J. Geryk, P. Trefil, J. Hejnar,

. 2018 ; 10 (11) : . [pub] 20181102

Language English Country Switzerland

Document type Journal Article, Research Support, Non-U.S. Gov't

Persistent link   https://www.medvik.cz/link/bmc19012176

Avian leukosis viruses (ALVs), which are pathogens of concern in domestic poultry, utilize specific receptor proteins for cell entry that are both necessary and sufficient for host susceptibility to a given ALV subgroup. This unequivocal relationship offers receptors as suitable targets of selection and biotechnological manipulation with the aim of obtaining virus-resistant poultry. This approach is further supported by the existence of natural knock-outs of receptor genes that segregate in inbred lines of chickens. We used CRISPR/Cas9 genome editing tools to introduce frame-shifting indel mutations into tva, tvc, and tvj loci encoding receptors for the A, C, and J ALV subgroups, respectively. For all three loci, the homozygous frame-shifting indels generating premature stop codons induced phenotypes which were fully resistant to the virus of respective subgroup. In the tvj locus, we also obtained in-frame deletions corroborating the importance of W38 and the four amino-acids preceding it. We demonstrate that CRISPR/Cas9-mediated knock-out or the fine editing of ALV receptor genes might be the first step in the development of virus-resistant chickens.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc19012176
003      
CZ-PrNML
005      
20190408082158.0
007      
ta
008      
190405s2018 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/v10110605 $2 doi
035    __
$a (PubMed)30400152
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Koslová, Anna $u Institute of Molecular Genetics, Czech Academy of Sciences, Videnska 1083, CZ 14220 Prague 4, Czech Republic. anna.koslova@mpimf-heidelberg.mpg.de.
245    10
$a Genetic Resistance to Avian Leukosis Viruses Induced by CRISPR/Cas9 Editing of Specific Receptor Genes in Chicken Cells / $c A. Koslová, D. Kučerová, M. Reinišová, J. Geryk, P. Trefil, J. Hejnar,
520    9_
$a Avian leukosis viruses (ALVs), which are pathogens of concern in domestic poultry, utilize specific receptor proteins for cell entry that are both necessary and sufficient for host susceptibility to a given ALV subgroup. This unequivocal relationship offers receptors as suitable targets of selection and biotechnological manipulation with the aim of obtaining virus-resistant poultry. This approach is further supported by the existence of natural knock-outs of receptor genes that segregate in inbred lines of chickens. We used CRISPR/Cas9 genome editing tools to introduce frame-shifting indel mutations into tva, tvc, and tvj loci encoding receptors for the A, C, and J ALV subgroups, respectively. For all three loci, the homozygous frame-shifting indels generating premature stop codons induced phenotypes which were fully resistant to the virus of respective subgroup. In the tvj locus, we also obtained in-frame deletions corroborating the importance of W38 and the four amino-acids preceding it. We demonstrate that CRISPR/Cas9-mediated knock-out or the fine editing of ALV receptor genes might be the first step in the development of virus-resistant chickens.
650    _2
$a zvířata $7 D000818
650    _2
$a ptačí leukóza $x genetika $x virologie $7 D001353
650    _2
$a virus ptačí leukózy $x fyziologie $7 D001354
650    _2
$a sekvence nukleotidů $7 D001483
650    12
$a CRISPR-Cas systémy $7 D064113
650    _2
$a buněčné linie $7 D002460
650    _2
$a kur domácí $7 D002645
650    _2
$a odolnost vůči nemocem $x genetika $7 D060467
650    12
$a editace genu $7 D000072669
650    _2
$a virové geny $7 D005814
650    _2
$a genetické techniky $7 D005821
650    _2
$a genetické vektory $x genetika $7 D005822
650    _2
$a guide RNA, Kinetoplastida $7 D017394
650    _2
$a virové receptory $x genetika $x metabolismus $7 D011991
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Kučerová, Dana $u Institute of Molecular Genetics, Czech Academy of Sciences, Videnska 1083, CZ 14220 Prague 4, Czech Republic. dana.kucerova@img.cas.cz.
700    1_
$a Reinišová, Markéta $u Institute of Molecular Genetics, Czech Academy of Sciences, Videnska 1083, CZ 14220 Prague 4, Czech Republic. marketa.reinisova@img.cas.cz.
700    1_
$a Geryk, Josef $u Institute of Molecular Genetics, Czech Academy of Sciences, Videnska 1083, CZ 14220 Prague 4, Czech Republic. geryk@img.cas.cz.
700    1_
$a Trefil, Pavel $u BIOPHARM, Research Institute of Biopharmacy and Veterinary Drugs, 254 49 Jílové u Prahy, Czech Republic. trefil@bri.cz.
700    1_
$a Hejnar, Jiří $u Institute of Molecular Genetics, Czech Academy of Sciences, Videnska 1083, CZ 14220 Prague 4, Czech Republic. hejnar@img.cas.cz.
773    0_
$w MED00177099 $t Viruses $x 1999-4915 $g Roč. 10, č. 11 (2018)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/30400152 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20190405 $b ABA008
991    __
$a 20190408082211 $b ABA008
999    __
$a ok $b bmc $g 1391486 $s 1050481
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2018 $b 10 $c 11 $e 20181102 $i 1999-4915 $m Viruses $n Viruses $x MED00177099
LZP    __
$a Pubmed-20190405

Find record

Citation metrics

Archiving options